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The phosphate transporter gene OsPht1;8 is involved in phosphate homeostasis in rice.
Jia H
,
Ren H
,
Gu M
,
Zhao J
,
Sun S
,
Zhang X
,
Chen J
,
Wu P
,
Xu G
.
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Plant phosphate transporters (PTs) are active in the uptake of inorganic phosphate (Pi) from the soil and its translocation within the plant. Here, we report on the biological properties and physiological roles of OsPht1;8 (OsPT8), one of the PTs belonging to the Pht1 family in rice (Oryza sativa). Expression of a β-glucuronidase and green fluorescent protein reporter gene driven by the OsPT8 promoter showed that OsPT8 is expressed in various tissue organs from roots to seeds independent of Pi supply. OsPT8 was able to complement a yeast Pi-uptake mutant and increase Pi accumulation of Xenopus laevis oocytes when supplied with micromolar (33)Pi concentrations at their external solution, indicating that it has a high affinity for Pi transport. Overexpression of OsPT8 resulted in excessive Pi in both roots and shoots and Pi toxic symptoms under the high-Pi supply condition. In contrast, knockdown of OsPT8 by RNA interference decreased Pi uptake and plant growth under both high- and low-Pi conditions. Moreover, OsPT8 suppression resulted in an increase of phosphorus content in the panicle axis and in a decrease of phosphorus content in unfilled grain hulls, accompanied by lower seed-setting rate. Altogether, our data suggest that OsPT8 is involved in Pi homeostasis in rice and is critical for plant growth and development.
Ai,
Two rice phosphate transporters, OsPht1;2 and OsPht1;6, have different functions and kinetic properties in uptake and translocation.
2009, Pubmed,
Xenbase
Ai,
Two rice phosphate transporters, OsPht1;2 and OsPht1;6, have different functions and kinetic properties in uptake and translocation.
2009,
Pubmed
,
Xenbase
Bucher,
Functional biology of plant phosphate uptake at root and mycorrhiza interfaces.
2007,
Pubmed
Bun-Ya,
The PHO84 gene of Saccharomyces cerevisiae encodes an inorganic phosphate transporter.
1991,
Pubmed
Campbell,
A monomeric red fluorescent protein.
2002,
Pubmed
Catarecha,
A mutant of the Arabidopsis phosphate transporter PHT1;1 displays enhanced arsenic accumulation.
2007,
Pubmed
Chen,
Conservation and divergence of both phosphate- and mycorrhiza-regulated physiological responses and expression patterns of phosphate transporters in solanaceous species.
2007,
Pubmed
Cleaver,
Overexpression of the tinman-related genes XNkx-2.5 and XNkx-2.3 in Xenopus embryos results in myocardial hyperplasia.
1996,
Pubmed
,
Xenbase
Daram,
Functional analysis and cell-specific expression of a phosphate transporter from tomato.
1998,
Pubmed
Glassop,
Cereal phosphate transporters associated with the mycorrhizal pathway of phosphate uptake into roots.
2005,
Pubmed
Goff,
A draft sequence of the rice genome (Oryza sativa L. ssp. japonica).
2002,
Pubmed
Güimil,
Comparative transcriptomics of rice reveals an ancient pattern of response to microbial colonization.
2005,
Pubmed
Harrison,
A phosphate transporter from Medicago truncatula involved in the acquisition of phosphate released by arbuscular mycorrhizal fungi.
2002,
Pubmed
Hu,
LEAF TIP NECROSIS1 plays a pivotal role in the regulation of multiple phosphate starvation responses in rice.
2011,
Pubmed
Leggewie,
Two cDNAs from potato are able to complement a phosphate uptake-deficient yeast mutant: identification of phosphate transporters from higher plants.
1997,
Pubmed
Liu,
Cloning and characterization of two phosphate transporters from Medicago truncatula roots: regulation in response to phosphate and to colonization by arbuscular mycorrhizal (AM) fungi.
1998,
Pubmed
Liu,
OsSPX1 suppresses the function of OsPHR2 in the regulation of expression of OsPT2 and phosphate homeostasis in shoots of rice.
2010,
Pubmed
Miao,
Transient expression of fluorescent fusion proteins in protoplasts of suspension cultured cells.
2007,
Pubmed
Misson,
Transcriptional regulation and functional properties of Arabidopsis Pht1;4, a high affinity transporter contributing greatly to phosphate uptake in phosphate deprived plants.
2004,
Pubmed
Mitsukawa,
Overexpression of an Arabidopsis thaliana high-affinity phosphate transporter gene in tobacco cultured cells enhances cell growth under phosphate-limited conditions.
1997,
Pubmed
Mudge,
Expression analysis suggests novel roles for members of the Pht1 family of phosphate transporters in Arabidopsis.
2002,
Pubmed
Nelson,
A multicolored set of in vivo organelle markers for co-localization studies in Arabidopsis and other plants.
2007,
Pubmed
Nilsson,
Increased expression of the MYB-related transcription factor, PHR1, leads to enhanced phosphate uptake in Arabidopsis thaliana.
2007,
Pubmed
Paszkowski,
A journey through signaling in arbuscular mycorrhizal symbioses 2006.
2006,
Pubmed
Paszkowski,
Rice phosphate transporters include an evolutionarily divergent gene specifically activated in arbuscular mycorrhizal symbiosis.
2002,
Pubmed
Poirier,
Phosphate transport and homeostasis in Arabidopsis.
2002,
Pubmed
Preuss,
Channel-like characteristics of the low-affinity barley phosphate transporter PHT1;6 when expressed in Xenopus oocytes.
2010,
Pubmed
,
Xenbase
Preuss,
Proton-coupled high-affinity phosphate transport revealed from heterologous characterization in Xenopus of barley-root plasma membrane transporter, HvPHT1;1.
2011,
Pubmed
,
Xenbase
Rae,
Characterization of two phosphate transporters from barley; evidence for diverse function and kinetic properties among members of the Pht1 family.
2003,
Pubmed
Raghothama,
Phosphate transport and signaling.
2000,
Pubmed
Raghothama,
PHOSPHATE ACQUISITION.
1999,
Pubmed
Rausch,
Expression analysis suggests novel roles for the plastidic phosphate transporter Pht2;1 in auto- and heterotrophic tissues in potato and Arabidopsis.
2004,
Pubmed
Rausch,
A phosphate transporter expressed in arbuscule-containing cells in potato.
2001,
Pubmed
Rausch,
Molecular mechanisms of phosphate transport in plants.
2002,
Pubmed
Rubio,
A conserved MYB transcription factor involved in phosphate starvation signaling both in vascular plants and in unicellular algae.
2001,
Pubmed
Schachtman,
Nutrient sensing and signaling: NPKS.
2007,
Pubmed
Shin,
Phosphate transport in Arabidopsis: Pht1;1 and Pht1;4 play a major role in phosphate acquisition from both low- and high-phosphate environments.
2004,
Pubmed
Tong,
A two-component high-affinity nitrate uptake system in barley.
2005,
Pubmed
,
Xenbase
Wang,
Involvement of OsSPX1 in phosphate homeostasis in rice.
2009,
Pubmed
Zhou,
OsPHR2 is involved in phosphate-starvation signaling and excessive phosphate accumulation in shoots of plants.
2008,
Pubmed