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Proc Natl Acad Sci U S A
2011 Jan 25;1084:1699-704. doi: 10.1073/pnas.1016114108.
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Ca2+-dependent structural rearrangements within Na+-Ca2+ exchanger dimers.
John SA
,
Ribalet B
,
Weiss JN
,
Philipson KD
,
Ottolia M
.
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Cytoplasmic Ca(2+) is known to regulate Na(+)-Ca(2+) exchanger (NCX) activity by binding to two adjacent Ca(2+)-binding domains (CBD1 and CBD2) located in the large intracellular loop between transmembrane segments 5 and 6. We investigated Ca(2+)-dependent movements as changes in FRET between exchanger proteins tagged with CFP or YFP at position 266 within the large cytoplasmic loop. Data indicate that the exchanger assembles as a dimer in the plasma membrane. Addition of Ca(2+) decreases the distance between the cytoplasmic loops of NCX pairs. The Ca(2+)-dependent movements detected between paired NCXs were abolished by mutating the Ca(2+) coordination sites in CBD1 (D421A, E451A, and D500V), whereas disruption of the primary Ca(2+) coordination site in CBD2 (E516L) had no effect. Thus, the Ca(2+)-induced conformational changes of NCX dimers arise from the movement of CBD1. FRET studies of CBD1, CBD2, and CBD1-CBD2 peptides displayed Ca(2+)-dependent movements with different apparent affinities. CBD1-CBD2 showed a Ca(2+)-dependent phenotype mirroring full-length NCX but distinct from both CBD1 and CBD2.
Besserer,
The second Ca2+-binding domain of the Na+ Ca2+ exchanger is essential for regulation: crystal structures and mutational analysis.
2007, Pubmed
Besserer,
The second Ca2+-binding domain of the Na+ Ca2+ exchanger is essential for regulation: crystal structures and mutational analysis.
2007,
Pubmed
Boyman,
Kinetic and equilibrium properties of regulatory calcium sensors of NCX1 protein.
2009,
Pubmed
Bykova,
Large movement in the C terminus of CLC-0 chloride channel during slow gating.
2006,
Pubmed
Chaptal,
Structure and functional analysis of a Ca2+ sensor mutant of the Na+/Ca2+ exchanger.
2009,
Pubmed
Farhan,
Oligomerization of neurotransmitter transporters: a ticket from the endoplasmic reticulum to the plasma membrane.
2006,
Pubmed
Fuster,
Steady-state function of the ubiquitous mammalian Na/H exchanger (NHE1) in relation to dimer coupling models with 2Na/2H stoichiometry.
2008,
Pubmed
Giladi,
Essential role of the CBD1-CBD2 linker in slow dissociation of Ca2+ from the regulatory two-domain tandem of NCX1.
2010,
Pubmed
Hilge,
Ca2+ regulation in the Na+/Ca2+ exchanger involves two markedly different Ca2+ sensors.
2006,
Pubmed
Hilge,
Ca2+ regulation in the Na+/Ca2+ exchanger features a dual electrostatic switch mechanism.
2009,
Pubmed
Hilgemann,
Steady-state and dynamic properties of cardiac sodium-calcium exchange. Sodium-dependent inactivation.
1992,
Pubmed
Hilgemann,
Steady-state and dynamic properties of cardiac sodium-calcium exchange. Secondary modulation by cytoplasmic calcium and ATP.
1992,
Pubmed
Hilgemann,
Unitary cardiac Na+, Ca2+ exchange current magnitudes determined from channel-like noise and charge movements of ion transport.
1996,
Pubmed
,
Xenbase
Johnson,
Structure and dynamics of Ca2+-binding domain 1 of the Na+/Ca2+ exchanger in the presence and in the absence of Ca2+.
2008,
Pubmed
Kenworthy,
Distribution of a glycosylphosphatidylinositol-anchored protein at the apical surface of MDCK cells examined at a resolution of <100 A using imaging fluorescence resonance energy transfer.
1998,
Pubmed
Kimura,
Na-Ca exchange current in mammalian heart cells.
,
Pubmed
Levitsky,
Identification of the high affinity Ca(2+)-binding domain of the cardiac Na(+)-Ca2+ exchanger.
1994,
Pubmed
Matsuoka,
Regulation of the cardiac Na(+)-Ca2+ exchanger by Ca2+. Mutational analysis of the Ca(2+)-binding domain.
1995,
Pubmed
,
Xenbase
Mercier,
Quantitative assessment of beta 1- and beta 2-adrenergic receptor homo- and heterodimerization by bioluminescence resonance energy transfer.
2002,
Pubmed
Nagai,
Expanded dynamic range of fluorescent indicators for Ca(2+) by circularly permuted yellow fluorescent proteins.
2004,
Pubmed
Nicoll,
The crystal structure of the primary Ca2+ sensor of the Na+/Ca2+ exchanger reveals a novel Ca2+ binding motif.
2006,
Pubmed
Ottolia,
Interactions between Ca2+ binding domains of the Na+-Ca2+ exchanger and secondary regulation.
2010,
Pubmed
Ottolia,
Conformational changes of the Ca(2+) regulatory site of the Na(+)-Ca(2+) exchanger detected by FRET.
2004,
Pubmed
Ottolia,
Fluorescent Na+-Ca+ exchangers: electrophysiological and optical characterization.
2007,
Pubmed
,
Xenbase
Ottolia,
Xenopus oocyte plasma membrane sheets for FRET analysis.
2007,
Pubmed
,
Xenbase
Ottolia,
Shedding light on the Na+/Ca2+ exchanger.
2007,
Pubmed
,
Xenbase
Ottolia,
Roles of two Ca2+-binding domains in regulation of the cardiac Na+-Ca2+ exchanger.
2009,
Pubmed
,
Xenbase
Patton,
Some precautions in using chelators to buffer metals in biological solutions.
2004,
Pubmed
Philipson,
The Na+/Ca2+ exchange molecule: an overview.
2002,
Pubmed
Reeves,
Allosteric activation of sodium-calcium exchange activity by calcium: persistence at low calcium concentrations.
2003,
Pubmed
Ren,
Intermolecular cross-linking of Na+-Ca2+ exchanger proteins: evidence for dimer formation.
2008,
Pubmed
Schwarzer,
The Na/Ca-K exchanger of rod photoreceptor exists as dimer in the plasma membrane.
1997,
Pubmed
Sitte,
Sodium-dependent neurotransmitter transporters: oligomerization as a determinant of transporter function and trafficking.
2004,
Pubmed
Stryer,
Fluorescence energy transfer as a spectroscopic ruler.
1978,
Pubmed
Van Munster,
Fluorescence resonance energy transfer (FRET) measurement by gradual acceptor photobleaching.
2005,
Pubmed
Wei,
Cytosolic free magnesium modulates Na/Ca exchange currents in pig myocytes.
2002,
Pubmed
Wolber,
An analytic solution to the Förster energy transfer problem in two dimensions.
1979,
Pubmed
Wu,
Crystal structure of CBD2 from the Drosophila Na(+)/Ca(2+) exchanger: diversity of Ca(2+) regulation and its alternative splicing modification.
2009,
Pubmed
Wu,
Crystal structures of progressive Ca2+ binding states of the Ca2+ sensor Ca2+ binding domain 1 (CBD1) from the CALX Na+/Ca2+ exchanger reveal incremental conformational transitions.
2010,
Pubmed
Xie,
Conformational changes of a Ca2+-binding domain of the Na+/Ca2+ exchanger monitored by FRET in transgenic zebrafish heart.
2008,
Pubmed
Zacharias,
Partitioning of lipid-modified monomeric GFPs into membrane microdomains of live cells.
2002,
Pubmed
Zhao,
Interaction between Arabidopsis Ca2+/H+ exchangers CAX1 and CAX3.
2009,
Pubmed
