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Methods Mol Biol January 1, 2011; 770 55-75.

Manipulation of gene function in Xenopus laevis.

Mimoto MS , Christian JL .

Xenopus laevis embryos are particularly well suited to address questions requiring either knockdown or overexpression of genes in a tissue-specific fashion during vertebrate embryonic development. These manipulations are achieved by targeted injection of either antisense morpholino oligonucleotides or synthetic mRNAs, respectively, into the early embryo. Herein we offer detailed protocols describing how to design and perform these experiments successfully, as well as a brief discussion of considerations for performing a microarray analysis in this organism.

PubMed ID: 21805261
PMC ID: PMC3911881
Article link: Methods Mol Biol
Grant support: [+]

References [+] :
Dale, Fate map for the 32-cell stage of Xenopus laevis. 1987, Pubmed, Xenbase