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Figure 1. Schematics for various stages of the nucleosome unwrapping.Nucleosome conformations are shown with different number of turns, rotation angle and length of wrapped DNA.
Figure 2. Representative AFM scans of nucleosome core particles.Nucleosomes were reconstituted using native histone H4 (a) or biotinylated histone K12Cbio-H4 (b). Images were acquired with NanoScope IIId AFM system operating in Tapping mode. Scan sizes are 0.5 µm.
Figure 3. Representative enlarged AFM scans of NCP.Nucleosomes were reconstituted using non-biotinylated native histone H4 (a) and biotinylated K12C histone H4 (b). The complexes are labeled with the number of DNA turns around histone octamers. Scans sizes are 200×200 nm.
Figure 4. Histograms for lengths of nucleosomal DNA (nsDNA) wrapped around histone cores.Nucleosomes were reconstituted using native H4 histone (a), K12C-H4 mutant (b) or K12Cbio-H4 histone (c). It can be seen that in nucleosomes made with K12Cbio-H4 wDNA is shifted towards higher value compared to samples reconstituted using non-biotinylated native H4 or K12C-H4 mutant. The mean values of nsDNA indicated with arrows were 49.8 nm ±1.5 nm, 48.8 nm ±1.4 nm and 56.6 nm ±1.1 nm for NCP containing native histone H4, K12C-H4 mutant, and K12Cbio-H4, respectively.
Figure 5. Model of the effect of biotinylation on conformation of nucleosome.Both front and top views are shown. The segment of the DNA arm that contributes to additional wrapping is shown in red.
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