Shafer ME et al. (2011), Expression analysis of the peroxiredoxin gene f...

XB-ART-43900

Gene Expr Patterns 2011 Dec 01;118:511-6. doi: 10.1016/j.gep.2011.08.007.

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Expression analysis of the peroxiredoxin gene family during early development in Xenopus laevis.

Shafer ME , Willson JA , Damjanovski S .

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Development in the frog, Xenopus laevis, requires the utilization of yolk glyco-lipo-proteins in a temporally- and spatially-dependent manner. The metabolism of the yolk produces hydrogen peroxide (H(2)O(2)), a potent reactive oxygen species (ROS). Peroxiredoxins (prdxs) are a family of six anti-oxidant enzymes that, amongst other roles, reduce H(2)O(2). Prdxs reduce H(2)O(2) through a thiol-redox reaction at conserved cysteine residues which results in the creation of disulfide bonds. Recently the thiol-redox reaction of Prdxs has also been implicated in several cell signaling systems. Here we report the cloning and expression patterns during development of six peroxiredoxin homologs from the frog X. laevis. Sequence analysis confirmed their identity as well as their evolutionary relationship with peroxiredoxins from several other species. Using RT-PCR and in situ hybridization analysis we have shown that there is early and robust expression of all six homologs during development. All six X. laevis peroxiredoxins are expressed in neural regions including the brain, eyes, as well as the somites. Different expression patterns for each peroxiredoxin are also observed in the pronephric region, including the proximal and distal tubules. Expression of several peroxiredoxins was also observed in the blood precursors and the olfactory placode. These results suggest important roles for all six peroxiredoxins during early development. These roles may be restricted to their functions as anti-oxidant enzymes, but may also be related to their emerging roles in redox signaling.

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Species referenced: Xenopus laevis
Genes referenced: prdx1 prdx2 prdx3 prdx4 prdx5 prdx6 uqcc6

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	Fig. 1. Expression profiles of X. laevis prdx mRNA during stages 18–20 and 23/24 of embryonic development. Whole mount in situ hybridization with antisense Digoxigenin-labeled probes against the coding regions of the X. laevis prdxs was performed to determine the spatial expression pattern of prdx1–6 at stages 18–20 (A–F) and 23/24 (G–L). (BR, brain; EY, presumptive eye; OP, optic placode; PN, pronephros; PA, pharyngeal arches; NT, neural tube; SM, somites; BP, blood precursors).
	Fig. 2. Expression profiles of X. laevis prdx mRNA during stages 27/28 and 33/34 of embryonic development. Whole mount in situ hybridization with antisense Digoxigenin-labeled probes against the coding regions of the X. laevis prdxs was performed to determine the spatial expression pattern of prdx1 at stages 27/28 (A-F) and 33/34 (G-L). The white lines in panel G (i, ii and iii) indicate the positions of the cuts made to give the cross-sections in Supplementary Fig. S4. (Br, brain; EY, eye; PN, pronephros; FB, forebrain; HB, hindbrain; PD, proctodeum; Li, liver; PT, proximal tubule; SM, somites; PA, pharyngeal arches; Bl, blood islands; Of, olfactory placode; DT, distal tubule; CT, connecting tubule). The horizontal line across the tails (in G) is a background artifact
	Fig. 3. Expression profiles of X. laevis prdx mRNA during stages 36/37 and 40/41 of embryonic development. Whole mount in situ hybridization with antisense Digoxigenin-labeled probes against the coding regions of the X. laevis prdxs was performed to determine the spatial expression pattern of prdx1 at stages 36/37 (A) and 40/41 (G). (Br, brain; PT, proximal tubule; Li, liver; PD, proctodeum; Ey, eye; Bl, blood islands; Vc, vasculature; PA, pharyngeal arches; PN, pronephros; SM, somites; Of, olfactory placode; CN, cranial nerves; DT, distal tubule; CT, connecting tubule; TG, thyroid gland). The horizontal line across the tails is a background artifact.