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Dev Dyn June 1, 2011; 240 (6): 1422-9.

Gsx transcription factors repress Iroquois gene expression.

Winterbottom EF , Ramsbottom SA , Isaacs HV .

We have previously shown that the Gsx family homeobox gene Gsh2 is part of the regulatory network specifying dorsoventral pattern of primary neurons in the developing amphibian embryo. Here, we investigate the role of Gsx transcription factors in regulating the transcription of Iroquois family homeobox genes in the amphibian neural plate. Iroquois genes are key regulators of neural patterning and their expression is coincident with that of the Gsx genes during open neural plate stages. We show that Gsx proteins repress Iroquois expression in the embryo and conversely, inhibition of Gsx activity with either antisense morpholino oligos or an anti-morphic Gsx protein up-regulates Iroquois expression. These data indicate that Gsx factors act as negative regulators of Iroquois gene expression in the amphibian neural plate and support a model in which the Gsx proteins promote neuronal differentiation by repressing the expression of known inhibitors of neuronal differentiation such as Iro3.

PubMed ID: 21538683
Article link: Dev Dyn

Species referenced: Xenopus
Genes referenced: bmp4 bmp7.1 bmp7.2 gal.2 gsx1 gsx2 irx2 irx3 nog odc1
Antibodies: Smad1 Ab8
Morpholinos: gsx1 MO1 gsx2 MO1

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