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XB-ART-44277
Dev Neurobiol August 1, 2010; 70 (9): 623-35.
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Cellular retinol binding protein 1 modulates photoreceptor outer segment folding in the isolated eye.

Wang X , Tong Y , Giorgianni F , Beranova-Giorgianni S , Penn JS , Jablonski MM .


Abstract
In a previous study, we used differential proteomics to identify retinal proteins whose steady-state levels were altered in an experimental system in which photoreceptor outer segments were improperly folded. We determined that the steady-state level of cellular retinol binding protein 1 (CRBP1) was downregulated in eyes lacking organized outer segments. The purpose of this study was to determine if CRBP1 is a plausible candidate for regulating outer segment assembly. We used Morpholinos to directly test the hypothesis that a decreased level of CRBP1 protein was associated with the misfolding of outer segments. Results from these studies indicate that downregulation of CRBP1 protein resulted in aberrant assembly of outer segments. Because CRBP1 plays a dual role in the retina-retinal recycling and generation of retinoic acid-we evaluated both possibilities. Our data demonstrate that outer segment folding was not modified by 11-cis retinal supplementation, suggesting that CRBP1 influences outer segment assembly through a mechanism unrelated to rhodopsin regeneration. In contrast, retinoic acid is required for the proper organization of nascent outer segment membranes. The localization of CRBP1 within Muller cells and the RPE and its demonstrated role in modulating the proper folding of nascent outer segment membranes through retinoic acid further elucidates the role of these cells in directly influencing photoreceptor physiology.

PubMed ID: 20506167
PMC ID: PMC3628621
Article link: Dev Neurobiol
Grant support: [+]

Species referenced: Xenopus laevis
Genes referenced: actl6a glul mag myh3 rbp1 rho rpe tbx2
Antibodies: Glul Ab1 Rbp1 Ab1
Morpholinos: rbp1 MO1


Article Images: [+] show captions
References [+] :
Araque, Glial modulation of synaptic transmission in culture. 2004, Pubmed