Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
EMBO J
2003 Nov 17;2222:6078-88. doi: 10.1093/emboj/cdg593.
Show Gene links
Show Anatomy links
An SP1-like transcription factor Spr2 acts downstream of Fgf signaling to mediate mesoderm induction.
Zhao J
,
Cao Y
,
Zhao C
,
Postlethwait J
,
Meng A
.
???displayArticle.abstract???
Fgf signaling, mediated in part by the transcription factor Brachyury/Xbra/Ntl, plays important roles in mesoderm formation during the early development of vertebrate embryos. We have identified a zebrafish gene, spr2, which encodes a member of the Sp1-like transcription factor family. spr2 is expressed in both hypoblast and epiblast cells during late blastulation/early gastrulation, and in some mesodermal and neural tissues at later stages. Injection with spr2 mRNA enhances ntl expression and alleviates the inhibitory effect on ntl of XFD, a Xenopus dominant-negative FGF receptor. In contrast, morpholino- mediated knockdown of Spr2 activity inhibits ntl expression and reduces the inductive effect of Fgfs on ntl. We also demonstrate that Fgf signaling relays mesoderm induction activity of Nodal signaling and Spr2 is involved in this signal relay process. Furthermore, the correct spatial expression of spr2 requires Nodal, Fgf and Wnt signals. We suggest that expression of spr2 is an immediate-early response to mesoderm induction by Fgfs, which in turn regulates the expression of effector genes involved in the development of mesodermal tissues.
Agius,
Endodermal Nodal-related signals and mesoderm induction in Xenopus.
2000, Pubmed,
Xenbase
Agius,
Endodermal Nodal-related signals and mesoderm induction in Xenopus.
2000,
Pubmed
,
Xenbase
Alexandre,
Ectopic expression of Hoxa-1 in the zebrafish alters the fate of the mandibular arch neural crest and phenocopies a retinoic acid-induced phenotype.
1996,
Pubmed
Amaya,
Expression of a dominant negative mutant of the FGF receptor disrupts mesoderm formation in Xenopus embryos.
1991,
Pubmed
,
Xenbase
Amaya,
FGF signalling in the early specification of mesoderm in Xenopus.
1993,
Pubmed
,
Xenbase
Baker,
Wnt signaling in Xenopus embryos inhibits bmp4 expression and activates neural development.
1999,
Pubmed
,
Xenbase
Chen,
The role of the yolk syncytial layer in germ layer patterning in zebrafish.
2000,
Pubmed
Clements,
Mode of action of VegT in mesoderm and endoderm formation.
1999,
Pubmed
,
Xenbase
Conlon,
A primary requirement for nodal in the formation and maintenance of the primitive streak in the mouse.
1994,
Pubmed
Conlon,
Inhibition of Xbra transcription activation causes defects in mesodermal patterning and reveals autoregulation of Xbra in dorsal mesoderm.
1996,
Pubmed
,
Xenbase
Cornell,
Activin-mediated mesoderm induction requires FGF.
1994,
Pubmed
,
Xenbase
Cunliffe,
Ectopic mesoderm formation in Xenopus embryos caused by widespread expression of a Brachyury homologue.
1992,
Pubmed
,
Xenbase
Detrich,
Intraembryonic hematopoietic cell migration during vertebrate development.
1995,
Pubmed
Feldman,
Zebrafish organizer development and germ-layer formation require nodal-related signals.
1998,
Pubmed
Griffin,
Analysis of FGF function in normal and no tail zebrafish embryos reveals separate mechanisms for formation of the trunk and the tail.
1995,
Pubmed
Gritsman,
The EGF-CFC protein one-eyed pinhead is essential for nodal signaling.
1999,
Pubmed
,
Xenbase
Halpern,
Induction of muscle pioneers and floor plate is distinguished by the zebrafish no tail mutation.
1993,
Pubmed
Hammerschmidt,
The expression of a zebrafish gene homologous to Drosophila snail suggests a conserved function in invertebrate and vertebrate gastrulation.
1993,
Pubmed
,
Xenbase
Harrison,
Sp5, a new member of the Sp1 family, is dynamically expressed during development and genetically interacts with Brachyury.
2000,
Pubmed
Herrmann,
Cloning of the T gene required in mesoderm formation in the mouse.
1990,
Pubmed
Isaacs,
eFGF regulates Xbra expression during Xenopus gastrulation.
1994,
Pubmed
,
Xenbase
Kaczynski,
Sp1- and Krüppel-like transcription factors.
2003,
Pubmed
Kadonaga,
Isolation of cDNA encoding transcription factor Sp1 and functional analysis of the DNA binding domain.
1987,
Pubmed
Kikuchi,
Roles of Axin in the Wnt signalling pathway.
1999,
Pubmed
Kimelman,
Synergistic induction of mesoderm by FGF and TGF-beta and the identification of an mRNA coding for FGF in the early Xenopus embryo.
1987,
Pubmed
,
Xenbase
Kimmel,
Origin and organization of the zebrafish fate map.
1990,
Pubmed
Kofron,
Mesoderm induction in Xenopus is a zygotic event regulated by maternal VegT via TGFbeta growth factors.
1999,
Pubmed
,
Xenbase
Krauss,
Expression of the zebrafish paired box gene pax[zf-b] during early neurogenesis.
1991,
Pubmed
LaBonne,
Mesoderm induction by activin requires FGF-mediated intracellular signals.
1994,
Pubmed
,
Xenbase
Latinkić,
The Xenopus Brachyury promoter is activated by FGF and low concentrations of activin and suppressed by high concentrations of activin and by paired-type homeodomain proteins.
1997,
Pubmed
,
Xenbase
Li,
Zebrafish sox9b is an early neural crest marker.
2002,
Pubmed
Nasevicius,
Effective targeted gene 'knockdown' in zebrafish.
2000,
Pubmed
Nieuwkoop,
The formation of the mesoderm in urodelean amphibians : I. Induction by the endoderm.
1969,
Pubmed
Ossipova,
XSPR-1 and XSPR-2, novel Sp1 related zinc finger containing genes, are dynamically expressed during Xenopus embryogenesis.
2002,
Pubmed
,
Xenbase
Reifers,
Fgf8 is mutated in zebrafish acerebellar (ace) mutants and is required for maintenance of midbrain-hindbrain boundary development and somitogenesis.
1998,
Pubmed
Rodaway,
Induction of the mesendoderm in the zebrafish germ ring by yolk cell-derived TGF-beta family signals and discrimination of mesoderm and endoderm by FGF.
1999,
Pubmed
Schier,
Nodal signalling in vertebrate development.
2000,
Pubmed
Schulte-Merker,
Mesoderm formation in response to Brachyury requires FGF signalling.
1995,
Pubmed
,
Xenbase
Schulte-Merker,
no tail (ntl) is the zebrafish homologue of the mouse T (Brachyury) gene.
1994,
Pubmed
Slack,
Mesoderm induction in early Xenopus embryos by heparin-binding growth factors.
,
Pubmed
,
Xenbase
Smith,
Expression of a Xenopus homolog of Brachyury (T) is an immediate-early response to mesoderm induction.
1991,
Pubmed
,
Xenbase
Sokol,
Wnt signaling and dorso-ventral axis specification in vertebrates.
1999,
Pubmed
Sun,
Targeted disruption of Fgf8 causes failure of cell migration in the gastrulating mouse embryo.
1999,
Pubmed
Tallafuss,
The zebrafish buttonhead-like factor Bts1 is an early regulator of pax2.1 expression during mid-hindbrain development.
2001,
Pubmed
Thisse,
Structure of the zebrafish snail1 gene and its expression in wild-type, spadetail and no tail mutant embryos.
1993,
Pubmed
Thisse,
Antivin, a novel and divergent member of the TGFbeta superfamily, negatively regulates mesoderm induction.
1999,
Pubmed
Treichel,
The novel transcription factor gene Sp5 exhibits a dynamic and highly restricted expression pattern during mouse embryogenesis.
2001,
Pubmed
Wilkinson,
Expression pattern of the mouse T gene and its role in mesoderm formation.
1990,
Pubmed
,
Xenbase
Wilson,
The status of Wnt signalling regulates neural and epidermal fates in the chick embryo.
2001,
Pubmed
,
Xenbase
Yamaguchi,
A PEA3 site flanked by SP1, SP4, and GATA sites positively regulates the differentiation-dependent expression of Brachyury in embryonal carcinoma P19 cells.
1999,
Pubmed
Zhang,
The role of maternal VegT in establishing the primary germ layers in Xenopus embryos.
1998,
Pubmed
,
Xenbase
Zhou,
Nodal is a novel TGF-beta-like gene expressed in the mouse node during gastrulation.
1993,
Pubmed
,
Xenbase
