XB-ART-44778Nat Cell Biol November 13, 2011; 13 (12): 1406-14.
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K-fibre minus ends are stabilized by a RanGTP-dependent mechanism essential for functional spindle assembly.
Chromosome segregation requires the formation of K-fibres, microtubule bundles that attach sister kinetochores to spindle poles. Most K-fibre microtubules originate around the chromosomes through a non-centrosomal RanGTP-dependent pathway and become oriented with the plus ends attached to the kinetochore and the minus ends focused at the spindle poles. The capture and stabilization of microtubule plus ends at the kinetochore has been extensively studied but very little is known on how their minus-end dynamics are controlled. Here we show that MCRS1 is a RanGTP-regulated factor essential for non-centrosomal microtubule assembly. MCRS1 localizes to the minus ends of chromosomal microtubules and K-fibres, where it protects them from depolymerization. Our data reveal the existence of a mechanism that stabilizes the minus ends of chromosomal microtubules and K-fibres, and is essential for the assembly of a functional bipolar spindle.
PubMed ID: 22081094
Article link: Nat Cell Biol
Species referenced: Xenopus
Genes referenced: mcrs1
References [+] :
Amaro, Molecular control of kinetochore-microtubule dynamics and chromosome oscillations. 2010, Pubmed