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Figure 1. The C-cadhering catenin binding domain (CBD) is required for cell adhesion . (A
) Immunostaining for C-cadherin (upper panels) and αEC (lower panels) in paraffin sections of stage 9 embryos, showing the non-rescue of cell adhesion in the absence of the CBD. (E) 3D projections of high magnification, and high resolution confocal images showing the localization of C-cadherin protein. Scale bars for A-D, 50 , E-H, 20 .
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Figure 2. C-terminus of α-E-catenin is sufficient to drive cadherin membrane expression.
(A) Immunostaining for C-cadherin (upper panels) and E-cadherin extracellular domain (lower panels) in animal caps, showing the rescue of cell adhesion and the expression of nEαC in cell junctions. (B) Immunostaining for β-catenin showing the junctional localization of nEαC is not through β-catenin. Scale bars for A-B, 50 .
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Figure 3. α-E-catenin is required for C-cadherin expression on the cell surface in the blastula.
(A) Immunostaining of animal caps stained for C-cadherin, showing that the loss of C-cadherin junctional localization caused by αEC depletion can be rescued by the expression of wild type αEC mRNA.(B) Quantification of cell junction-localized C-cadherin levels. (C) Immunostaining of animal caps stained for C-cadherin, showing that the loss of C-cadherin junctional localization caused by αEC depletion cannot be fully rescued by the injection of C-cadherin mRNA.(D) Wound healing assay of embryos in which the animal caps have been dissected out and healed for 1 hr in 1MR, showing the increased negative effect of both α-catenin depletion and C-cadherin over-expression. Scale bars in A, C 50 .
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Figure 4. The distribution of α-N-catenin and α-E-catenin mRNAs in the neural and non-neural ectoderm.
(A-C) αNC In-situ staining of st.139 embryos (D-F) αEC In-situ staining of st.139 embryos. NE (Neural Ectoderm), n-NE (non-Neural Ectoderm)
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Figure 5. Antisense morpholino mediated depletion of α-E-catenin in the non-Neural Ectoderm.
(A) Western blot showing the level of αEC, C-cadherin, and E-cadherin protein levels in uninjected and αEC-MO-injected embryos at st.9,11, and 19.(B-D) Confocal projections of the non-neural ectoderm of wild type (B) or αEC-depleted (C) st. 19 embryos, stained for C-cadherin (Red) and αEC (Green), and rescue of C-cadherin (Red) junctional localization by the introduction of Myc-tagged (green), human wild type αEC (D). (E) Quantification of pixel intensity for C-cadherin levels. (F) E-cadherin (Red) junctional localization in αEC-depleted cells (Green, GFP) in the non-neural ectoderm. (G-H) Cross sections of αEC-depleted (G), or uninjected (H) embryos, stained for E-cadherin (Red) and GFP (green). (I) Quantification of pixel intensity for E-cadherin levels. (J-K) Wholemount vibratome sections stained for E-cadherin in wildtype (J) or αEC-depleted (K) embryos. Scale bars in B-D, F, J-K 50 , G-H 100 .
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Figure 6. Depletion of α-E-catenin and α-N-catenin in the neural ectoderm.
(A) Neural plates containing αEC-depleted clones (green) stained for C-cadherin (Red) and GFP (Green). (B) Neural plates containing αNC-depleted clones (morpholino-injected side marked by *, and uninjected side marked by # ), stained for αNC (Red) and C-cadherin (Green). (C) αEC-depleted neural plate cells (Green, FLDX) stained for N-cadherin (Red). (D) αNC-depleted neural plate cells (Green, FLDX) stained for N-cadherin (Red). Scale bars in A-D, 50 .
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Figure 7. α-N-catenin is critical for the normal morphogenesis of the neural tube.
(A) Robust open neural plate phenotype observed in αNC morpholino-injected embryos. (B) RLDX fluorescent signal marking the descendents of αNC morpholino-injected cells of embryos shown in (A). (C) RLDX fluorescent signal marking the descendents of αNC morpholino and rescued mRNA-injected cells, for embryos shown in (D, first frame). (D) First and last frames of a timelapse movie taken at 5 minute intervals, showing the rescue of neurulation movements by sequentially injecting αNC mRNA into morpholino-injected cells. (E) Delay in neurulation movements observed in αEC-depleted neural plates. (F) RLDX fluorescent signal marking the descendents of αEC morpholino-injected cells of embryos shown in (E). (G) Embryos depleted of both αEC and αNC, showing cell dissociation in their neural plates. (H) RLDX fluorescent signal marking the descendents of αEC and αNC morpholino-injected cells for embryos shown in (G).
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Figure 8. Cortical F-actin staining in alpha catenin-depleted neural and non-neural ectodermal cells.
(A) Alexa-488 phalloidin staining for F-actin (green) in uninjected non-neural ectoderm cells. (B) αEC morpholino-injected non-neural ectoderm cells (Red, RLDX) showing the loss of F-actin (green). (C) Mean F-actin pixel intensity quantitation in uninjected and αEC-depleted non-neural ectoderm cells. (D) F-actin staining (green) in αEC-depleted neural ectoderm cells (Red). (E) Mean F-actin pixel intensity quantitation in uninjected and αEC-depleted neural ectoderm cells. (F) F-actin staining (green) in αNC-depleted neural ectoderm cells (Red). (G) Mean F-actin pixel intensity quantitation in uninjected and αNC-depleted neural ectoderm cells. (H) Neural plates stained for F-actin (green) in αEC, and αNC-depleted neural ectoderm cells (red). Scale bars, 50 .
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Figure 9. Co-Immunoprecipitations of cadherin-catenin complexes.
(A) Western blot data of immunoprecipitations of the C-, and E-, cadherin complexes, using HA-tagged C-, and E-, cadherin, and Myc-tagged α-E-catenin, expressed in the non-neural ectoderm. (B) Western blot data of duplicate E-cadherin immunoprecipitations on wild type or αEC-depleted embryos. (C) Western blot data of immunoprecipitating N-cadherin-Myc or αNC-HA expressed in the neural ectoderm. (D) Sequential immunoprecipitation of N-cadherin-Myc from the lystae of N-cadherin-Myc + αNC-HA mRNA-injected embryos from (C). (E) Replicate immunoprecipitations of N-cadherin-Myc in embryos expressing N-cadherin-Myc in their neural ectoderms. (F) Replicate immunoprecipitations of N-cadherin-Myc in embryos expressing N-cadherin-Myc in their non neural ectoderms.
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Figure 10. Three-dimensional projections of high resolution confocal images of cleared non-neural ectodermal cells.
(A) E-cadherin (green) and αEC (red) staining in wholemount, cleared, non-neural ectoderm samples imaged at 100in nyquist limit and using a 2zoom. (B) C-cadherin (green) and αEC (red) staining.(C) Side view projection of a 3D image stack from a single cell-cell contact membrane, E-cadherin (green) and αEC (red). (D) Side view projection of a 3D image stack from a single cell-cell contact membrane, C-cadherin (green) and αEC (red). Scale bars in A-B, 15 , C-D, 5 .
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Figure 11. E-cadherin requires α-E-catenin in the non-polarized blastocelic roof cells, but not in the polarized superficial animal cap cells for junctional localization and cell adhesion.
(A-C) Blastocelic roof cells of st. 9 animal caps stained for C-cadherin (red in A and B), or E-cadherin-HA (red in C), αEC (purple), and F-actin (green) in uninjected (A), αEC-depleted (B), or αEC + E-cadherin-HA mRNA injected (C) embryos. (D-E) Superficial cells imaged from the blastocelic cavity in animal caps where all the deeper cells have fallen off, stained for C-cadherin in (D), or E-cadherin (E), in αEC depleted (D), or αEC depleted + E-cadherin-HA mRNA-injected (E) embryos. (F) Western blot data showing the depletion of αEC protein and the expression of C-, and E-, cadherin protein levels in the experiment. Scale bars, 50 .
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