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J Bacteriol
2012 Dec 01;19424:6828-36. doi: 10.1128/JB.01665-12.
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Aquaporin AqpZ is involved in cell volume regulation and sensitivity to osmotic stress in Synechocystis sp. strain PCC 6803.
Akai M
,
Onai K
,
Morishita M
,
Mino H
,
Shijuku T
,
Maruyama H
,
Arai F
,
Itoh S
,
Hazama A
,
Checchetto V
,
Szabò I
,
Yukutake Y
,
Suematsu M
,
Yasui M
,
Ishiura M
,
Uozumi N
.
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The moderately halotolerant cyanobacterium Synechocystis sp. strain PCC 6803 contains a plasma membrane aquaporin, AqpZ. We previously reported that AqpZ plays a role in glucose metabolism under photomixotrophic growth conditions, suggesting involvement of AqpZ in cytosolic osmolarity homeostasis. To further elucidate the physiological role of AqpZ, we have studied its gene expression profile and its function in Synechocystis. The expression level of aqpZ was regulated by the circadian clock. AqpZ activity was insensitive to mercury in Xenopus oocytes and in Synechocystis, indicating that the AqpZ can be categorized as a mercury-insensitive aquaporin. Stopped-flow light-scattering spectrophotometry showed that addition of sorbitol and NaCl led to a slower decrease in cell volume of the Synechocystis ΔaqpZ strain than the wild type. The ΔaqpZ cells were more tolerant to hyperosmotic shock by sorbitol than the wild type. Consistent with this, recovery of oxygen evolution after a hyperosmotic shock by sorbitol was faster in the ΔaqpZ strain than in the wild type. In contrast, NaCl stress had only a small effect on oxygen evolution. The amount of AqpZ protein remained unchanged by the addition of sorbitol but decreased after addition of NaCl. This decrease is likely to be a mechanism to alleviate the effects of high salinity on the cells. Our results indicate that Synechocystis AqpZ functions as a water transport system that responds to daily oscillations of intracellular osmolarity.
Akai,
Plasma membrane aquaporin AqpZ protein is essential for glucose metabolism during photomixotrophic growth of Synechocystis sp. PCC 6803.
2011, Pubmed
Akai,
Plasma membrane aquaporin AqpZ protein is essential for glucose metabolism during photomixotrophic growth of Synechocystis sp. PCC 6803.
2011,
Pubmed
Allakhverdiev,
Inactivation of photosystems I and II in response to osmotic stress in Synechococcus. Contribution of water channels.
2000,
Pubmed
Allakhverdiev,
Ionic and osmotic effects of NaCl-induced inactivation of photosystems I and II in Synechococcus sp.
2000,
Pubmed
Aoki,
Circadian expression of the dnaK gene in the cyanobacterium Synechocystis sp. strain PCC 6803.
1995,
Pubmed
Asada,
Production and scavenging of reactive oxygen species in chloroplasts and their functions.
2006,
Pubmed
Azad,
Functional characterization and hyperosmotic regulation of aquaporin in Synechocystis sp. PCC 6803.
2011,
Pubmed
Berry,
Potassium uptake in the unicellular cyanobacterium Synechocystis sp. strain PCC 6803 mainly depends on a Ktr-like system encoded by slr1509 (ntpJ).
2003,
Pubmed
Blumwald,
Studies of osmoregulation in salt adaptation of cyanobacteria with ESR spin-probe techniques.
1983,
Pubmed
Blumwald,
Na+/H+ exchange in the cyanobacterium Synechococcus 6311.
1984,
Pubmed
Blumwald,
Salt adaptation of the cyanobacterium synechococcus 6311 growing in a continuous culture (turbidostat).
1984,
Pubmed
Daniels,
Characterization of a new vacuolar membrane aquaporin sensitive to mercury at a unique site.
1996,
Pubmed
Delamarche,
Visualization of AqpZ-mediated water permeability in Escherichia coli by cryoelectron microscopy.
1999,
Pubmed
Elanskaya,
Functional analysis of the Na+/H+ antiporter encoding genes of the cyanobacterium Synechocystis PCC 6803.
2002,
Pubmed
Froger,
Functional characterization of a microbial aquaglyceroporin.
2001,
Pubmed
,
Xenbase
Guenther,
Phosphorylation of soybean nodulin 26 on serine 262 enhances water permeability and is regulated developmentally and by osmotic signals.
2003,
Pubmed
,
Xenbase
Hagemann,
The ggtA gene encodes a subunit of the transport system for the osmoprotective compound glucosylglycerol in Synechocystis sp. strain PCC 6803.
1997,
Pubmed
Hagemann,
Molecular biology of cyanobacterial salt acclimation.
2011,
Pubmed
Haimovich-Dayan,
Cross-talk between photomixotrophic growth and CO(2) -concentrating mechanism in Synechocystis sp. strain PCC 6803.
2011,
Pubmed
Inaba,
Functional expression in Escherichia coli of low-affinity and high-affinity Na(+)(Li(+))/H(+) antiporters of Synechocystis.
2001,
Pubmed
Ishiura,
Expression of a gene cluster kaiABC as a circadian feedback process in cyanobacteria.
1998,
Pubmed
Iwabuchi,
Mechanosensitive ion channels in chara: influence of water channel inhibitors, HgCl2 and ZnCl2, on generation of receptor potential.
2008,
Pubmed
Kucho,
Global analysis of circadian expression in the cyanobacterium Synechocystis sp. strain PCC 6803.
2005,
Pubmed
Latifi,
Oxidative stress in cyanobacteria.
2009,
Pubmed
Marin,
Osmotic stress in Synechocystis sp. PCC 6803: low tolerance towards nonionic osmotic stress results from lacking activation of glucosylglycerol accumulation.
2006,
Pubmed
Marin,
Gene expression profiling reflects physiological processes in salt acclimation of Synechocystis sp. strain PCC 6803.
2004,
Pubmed
Matsuda,
Na+-dependent K+ uptake Ktr system from the cyanobacterium Synechocystis sp. PCC 6803 and its role in the early phases of cell adaptation to hyperosmotic shock.
2004,
Pubmed
Matsuo,
A systematic forward genetic analysis identified components of the Chlamydomonas circadian system.
2008,
Pubmed
Maurel,
Functional characterization of the Escherichia coli glycerol facilitator, GlpF, in Xenopus oocytes.
1994,
Pubmed
,
Xenbase
Maurel,
The vacuolar membrane protein gamma-TIP creates water specific channels in Xenopus oocytes.
1993,
Pubmed
,
Xenbase
Mikkat,
Uptake and use of the osmoprotective compounds trehalose, glucosylglycerol, and sucrose by the cyanobacterium Synechocystis sp. PCC6803
1997,
Pubmed
Mikkat,
Uptake and use of the osmoprotective compounds trehalose, glucosylglycerol, and sucrose by the cyanobacterium Synechocystis sp. PCC6803.
1997,
Pubmed
Miller,
Na+ requirement for growth, photosynthesis, and pH regulation in the alkalotolerant cyanobacterium Synechococcus leopoliensis.
1984,
Pubmed
Onai,
Circadian rhythms in the thermophilic cyanobacterium Thermosynechococcus elongatus: compensation of period length over a wide temperature range.
2004,
Pubmed
Preston,
The mercury-sensitive residue at cysteine 189 in the CHIP28 water channel.
1993,
Pubmed
,
Xenbase
Shapiguzov,
Osmotic shrinkage of cells of Synechocystis sp. PCC 6803 by water efflux via aquaporins regulates osmostress-inducible gene expression.
2005,
Pubmed
Stanier,
Purification and properties of unicellular blue-green algae (order Chroococcales).
1971,
Pubmed
Stirnberg,
A membrane-bound FtsH protease is involved in osmoregulation in Synechocystis sp. PCC 6803: the compatible solute synthesizing enzyme GgpS is one of the targets for proteolysis.
2007,
Pubmed
Tournaire-Roux,
Cytosolic pH regulates root water transport during anoxic stress through gating of aquaporins.
2003,
Pubmed
,
Xenbase
Trudel,
Central clock excites vasopressin neurons by waking osmosensory afferents during late sleep.
2010,
Pubmed
Tsukaguchi,
Molecular characterization of a broad selectivity neutral solute channel.
1998,
Pubmed
,
Xenbase
Tsunekawa,
Identification and characterization of the Na+/H+ antiporter Nhas3 from the thylakoid membrane of Synechocystis sp. PCC 6803.
2009,
Pubmed
Waditee,
Overexpression of a Na+/H+ antiporter confers salt tolerance on a freshwater cyanobacterium, making it capable of growth in sea water.
2002,
Pubmed
Yamada,
A family of transcripts encoding water channel proteins: tissue-specific expression in the common ice plant.
1995,
Pubmed
,
Xenbase
Yasui,
Rapid gating and anion permeability of an intracellular aquaporin.
1999,
Pubmed
,
Xenbase
Yukutake,
Mercury chloride decreases the water permeability of aquaporin-4-reconstituted proteoliposomes.
2008,
Pubmed
,
Xenbase
Zeidel,
Reconstitution of functional water channels in liposomes containing purified red cell CHIP28 protein.
1992,
Pubmed
,
Xenbase
Zeidel,
Ultrastructure, pharmacologic inhibition, and transport selectivity of aquaporin channel-forming integral protein in proteoliposomes.
1994,
Pubmed
Zhao,
Sequential effects of sodium depletion on photosystem II in Synechocystis.
1988,
Pubmed
