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Mol Biol Rep
2012 Dec 01;3912:10557-64. doi: 10.1007/s11033-012-1941-0.
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Molecular cloning and functional characterization of swine sodium dependent phosphate cotransporter type II b (NaPi-IIb) gene.
Zhifeng X
,
Rejun F
,
Longchang H
,
Wenqing S
.
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A sodium-dependent phosphate transporter gene, NaPi-IIb, was isolated from swine small intestine using cDNA library screening method. Sequencing analysis revealed that the NaPi-IIb cDNA sequences was 2,016 bp in length and encoded an open-reading frame consisting of 671 amino acids. The cDNA showed 83.1 % sequences identity to the human NaPi-IIb and 78.7 % sequences identity to the chicken NaPi-IIb. Prediction of membrane spanning domains based on the hydrophilic and hydrophobic properties of the amino acids suggested that a putative protein had nine transmembrane domains, with both the NH(2) and COOH terminal being intracellular. By northern blot, a ~4.2 kb transcript was found to be abundantly expressed in mallintestine, lung, ovary, mammary glands, liver, kidney, salivary glands, placenta and thymus. Microinjection of swine NaPi-IIb cRNA into Xenopus oocytes demonstrated that the NaPi-IIb showed sodium-dependent Pi cotransport activity, and an approximate 31-fold increase of Pi uptake was seen in cRNA injected oocytes. The swine NaPi-IIb transporter expressed in Xenopus oocytes had a Km for Pi of ~79.35 ± 7.2 μM. Furthermore, the pH dependency characterization of swine NaPi-IIb transporter showed activation at extracellular alkaline-pH.
Arima,
Molecular cloning of murine sodium-phosphate cotransporter type IIb (Na/P(i)-IIb) gene promoter and characterization of gene structure.
2000, Pubmed
Arima,
Molecular cloning of murine sodium-phosphate cotransporter type IIb (Na/P(i)-IIb) gene promoter and characterization of gene structure.
2000,
Pubmed
Arima,
Glucocorticoid regulation and glycosylation of mouse intestinal type IIb Na-P(i) cotransporter during ontogeny.
2002,
Pubmed
Berndt,
Novel mechanisms in the regulation of phosphorus homeostasis.
2009,
Pubmed
Biber,
Renal Na/Pi-cotransporters.
1996,
Pubmed
Chong,
Cloning, genetic mapping, and expression analysis of a mouse renal sodium-dependent phosphate cotransporter.
1995,
Pubmed
Chong,
Molecular cloning of the cDNA encoding a human renal sodium phosphate transport protein and its assignment to chromosome 6p21.3-p23.
1993,
Pubmed
Clerici,
Sodium-dependent phosphate and alanine transports but sodium-independent hexose transport in type II alveolar epithelial cells in primary culture.
1991,
Pubmed
Fanning,
Protein-protein interactions: PDZ domain networks.
1996,
Pubmed
Graham,
Characterization of a type IIb sodium-phosphate cotransporter from zebrafish (Danio rerio) kidney.
2003,
Pubmed
,
Xenbase
Hilfiker,
Characterization of a murine type II sodium-phosphate cotransporter expressed in mammalian small intestine.
1998,
Pubmed
,
Xenbase
Karim-Jimenez,
Molecular determinants for apical expression of the renal type IIa Na+/Pi-cotransporter.
2001,
Pubmed
Kavanaugh,
Identification and characterization of a widely expressed phosphate transporter/retrovirus receptor family.
1996,
Pubmed
Kavanaugh,
Cell-surface receptors for gibbon ape leukemia virus and amphotropic murine retrovirus are inducible sodium-dependent phosphate symporters.
1994,
Pubmed
,
Xenbase
Kuwahara,
Identification and functional analysis of a splice variant of mouse sodium-dependent phosphate transporter Npt2c.
2012,
Pubmed
,
Xenbase
Köhler,
Identification of functionally important sites in the first intracellular loop of the NaPi-IIa cotransporter.
2002,
Pubmed
,
Xenbase
Lambert,
Cysteine mutagenesis reveals novel structure-function features within the predicted third extracellular loop of the type IIa Na(+)/P(i) cotransporter.
2001,
Pubmed
,
Xenbase
Li,
Molecular cloning of two rat Na+/Pi cotransporters: evidence for differential tissue expression of transcripts.
1995,
Pubmed
Magagnin,
Expression cloning of human and rat renal cortex Na/Pi cotransport.
1993,
Pubmed
,
Xenbase
Miyamoto,
Cloning and functional expression of a Na(+)-dependent phosphate co-transporter from human kidney: cDNA cloning and functional expression.
1995,
Pubmed
,
Xenbase
Miyamoto,
Sodium-dependent phosphate cotransporters: lessons from gene knockout and mutation studies.
2011,
Pubmed
Murer,
The sodium phosphate cotransporter family SLC34.
2004,
Pubmed
Murer,
Proximal tubular phosphate reabsorption: molecular mechanisms.
2000,
Pubmed
Murer,
Renal brush border membrane Na/Pi-cotransport: molecular aspects in PTH-dependent and dietary regulation.
1996,
Pubmed
Olah,
The cellular receptor for gibbon ape leukemia virus is a novel high affinity sodium-dependent phosphate transporter.
1994,
Pubmed
Pribanic,
Interactions of MAP17 with the NaPi-IIa/PDZK1 protein complex in renal proximal tubular cells.
2003,
Pubmed
Sabbagh,
Intestinal npt2b plays a major role in phosphate absorption and homeostasis.
2009,
Pubmed
Saddoris,
Sodium-dependent phosphate uptake in the jejunum is post-transcriptionally regulated in pigs fed a low-phosphorus diet and is independent of dietary calcium concentration.
2010,
Pubmed
Segawa,
Type IIc sodium-dependent phosphate transporter regulates calcium metabolism.
2009,
Pubmed
Segawa,
Effect of hydrolysis-resistant FGF23-R179Q on dietary phosphate regulation of the renal type-II Na/Pi transporter.
2003,
Pubmed
Segawa,
Intestinal Na-P(i) cotransporter adaptation to dietary P(i) content in vitamin D receptor null mice.
2004,
Pubmed
Traebert,
Investigating the surface expression of the renal type IIa Na+/Pi-cotransporter in Xenopus laevis oocytes.
2001,
Pubmed
,
Xenbase
Werner,
Cloning and expression of cDNA for a Na/Pi cotransport system of kidney cortex.
1991,
Pubmed
,
Xenbase
Werner,
Evolution of the Na-P(i) cotransport systems.
2001,
Pubmed
,
Xenbase
Xu,
Sodium-inorganic phosphate cotransporter NaPi-IIb in the epididymis and its potential role in male fertility studied in a transgenic mouse model.
2003,
Pubmed
Xu,
Molecular cloning, functional characterization, tissue distribution, and chromosomal localization of a human, small intestinal sodium-phosphate (Na+-Pi) transporter (SLC34A2).
1999,
Pubmed
,
Xenbase
Xu,
Age-dependent regulation of rat intestinal type IIb sodium-phosphate cotransporter by 1,25-(OH)(2) vitamin D(3).
2002,
Pubmed
Yamamoto,
Hereditary hypophosphatemic rickets with hypercalciuria: a study for the phosphate transporter gene type IIc and osteoblastic function.
2007,
Pubmed
Yan,
Characterization of the chicken small intestine type IIb sodium phosphate cotransporter.
2007,
Pubmed
