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J Biol Chem
2006 Jun 16;28124:16279-88. doi: 10.1074/jbc.M600682200.
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Analysis of nucleosome repositioning by yeast ISWI and Chd1 chromatin remodeling complexes.
Stockdale C
,
Flaus A
,
Ferreira H
,
Owen-Hughes T
.
???displayArticle.abstract??? ISWI proteins form the catalytic core of a subset of ATP-dependent chromatin remodeling activities in eukaryotes from yeast to man. Many of these complexes have been found to reposition nucleosomes but with different directionalities. We find that the yeast Isw1a, Isw2, and Chd1 enzymes preferentially move nucleosomes toward more central locations on short DNA fragments whereas Isw1b does not. Importantly, the inherent positioning properties of the DNA play an important role in determining where nucleosomes are relocated to by all of these enzymes. However, a key difference is that the Isw1a, Isw2, and Chd1 enzymes are unable to move nucleosomes to positions closer than 15 bp from a DNA end, whereas Isw1b can. We also find that there is a correlation between the inability of enzymes to move nucleosomes close to DNA ends and the preferential binding to nucleosomes bearing linker DNA. These observations suggest that the accessibility of linker DNA together with the positioning properties of the underlying DNA play important roles in determining the outcome of remodeling by these enzymes.
Alén,
A role for chromatin remodeling in transcriptional termination by RNA polymerase II.
2002, Pubmed
Alén,
A role for chromatin remodeling in transcriptional termination by RNA polymerase II.
2002,
Pubmed
Beard,
Mobility of histones on the chromosome of simian virus 40.
1978,
Pubmed
Becker,
ATP-dependent nucleosome remodeling.
2002,
Pubmed
Brehm,
dMi-2 and ISWI chromatin remodelling factors have distinct nucleosome binding and mobilization properties.
2000,
Pubmed
Bruno,
Histone H2A/H2B dimer exchange by ATP-dependent chromatin remodeling activities.
2003,
Pubmed
,
Xenbase
Bruno,
Site-specific attachment of reporter compounds to recombinant histones.
2004,
Pubmed
Davey,
Solvent mediated interactions in the structure of the nucleosome core particle at 1.9 a resolution.
2002,
Pubmed
,
Xenbase
Dillingham,
Demonstration of unidirectional single-stranded DNA translocation by PcrA helicase: measurement of step size and translocation speed.
2000,
Pubmed
Drew,
Can one measure the free energy of binding of the histone octamer to different DNA sequences by salt-dependent reconstitution?
1991,
Pubmed
Eberharter,
Acf1, the largest subunit of CHRAC, regulates ISWI-induced nucleosome remodelling.
2001,
Pubmed
Elfring,
Identification and characterization of Drosophila relatives of the yeast transcriptional activator SNF2/SWI2.
1994,
Pubmed
Fazzio,
Chromatin remodeling in vivo: evidence for a nucleosome sliding mechanism.
2003,
Pubmed
Flaus,
Base-pair resolution mapping of nucleosome positions using site-directed hydroxy radicals.
1999,
Pubmed
Flaus,
Dynamic properties of nucleosomes during thermal and ATP-driven mobilization.
2003,
Pubmed
Flaus,
Mapping nucleosome position at single base-pair resolution by using site-directed hydroxyl radicals.
1996,
Pubmed
Hamiche,
ATP-dependent histone octamer sliding mediated by the chromatin remodeling complex NURF.
1999,
Pubmed
Kagalwala,
Topography of the ISW2-nucleosome complex: insights into nucleosome spacing and chromatin remodeling.
2004,
Pubmed
Kang,
GAL4 directs nucleosome sliding induced by NURF.
2002,
Pubmed
Kassabov,
SWI/SNF unwraps, slides, and rewraps the nucleosome.
2003,
Pubmed
,
Xenbase
Lorch,
Activated RSC-nucleosome complex and persistently altered form of the nucleosome.
1998,
Pubmed
Lowary,
New DNA sequence rules for high affinity binding to histone octamer and sequence-directed nucleosome positioning.
1998,
Pubmed
Luger,
Characterization of nucleosome core particles containing histone proteins made in bacteria.
1997,
Pubmed
,
Xenbase
Lusser,
Distinct activities of CHD1 and ACF in ATP-dependent chromatin assembly.
2005,
Pubmed
Längst,
Nucleosome movement by CHRAC and ISWI without disruption or trans-displacement of the histone octamer.
1999,
Pubmed
Meersseman,
Mobile nucleosomes--a general behavior.
1992,
Pubmed
Mizuguchi,
ATP-driven exchange of histone H2AZ variant catalyzed by SWR1 chromatin remodeling complex.
2004,
Pubmed
Morillon,
Isw1 chromatin remodeling ATPase coordinates transcription elongation and termination by RNA polymerase II.
2003,
Pubmed
Rigaut,
A generic protein purification method for protein complex characterization and proteome exploration.
1999,
Pubmed
Schnitzler,
Human SWI/SNF interconverts a nucleosome between its base state and a stable remodeled state.
1998,
Pubmed
Tran,
The chromo domain protein chd1p from budding yeast is an ATP-dependent chromatin-modifying factor.
2000,
Pubmed
Tsukiyama,
The in vivo functions of ATP-dependent chromatin-remodelling factors.
2002,
Pubmed
Tsukiyama,
Characterization of the imitation switch subfamily of ATP-dependent chromatin-remodeling factors in Saccharomyces cerevisiae.
1999,
Pubmed
Vary,
Yeast Isw1p forms two separable complexes in vivo.
2003,
Pubmed
Zofall,
Functional role of extranucleosomal DNA and the entry site of the nucleosome in chromatin remodeling by ISW2.
2004,
Pubmed
