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PLoS One
2013 Jan 01;81:e55585. doi: 10.1371/journal.pone.0055585.
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Tissue-specific upregulation of MDS/EVI gene transcripts in the intestine by thyroid hormone during Xenopus metamorphosis.
Miller TC
,
Sun G
,
Hasebe T
,
Fu L
,
Heimeier RA
,
Das B
,
Ishizuya-Oka A
,
Shi YB
.
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Intestinal remodeling during amphibian metamorphosis resembles the maturation of the adult intestine during mammalian postembryonic development when the adult epithelial self-renewing system is established under the influence of high concentrations of plasma thyroid hormone (T3). This process involves de novo formation and subsequent proliferation and differentiation of the adult stem cells. The T3-dependence of the formation of adult intestinal stem cell during Xenopus laevis metamorphosis offers a unique opportunity to identify genes likely important for adult organ-specific stem cell development. We have cloned and characterized the ectopic viral integration site 1 (EVI) and its variant myelodysplastic syndrome 1 (MDS)/EVI generated via transcription from the upstream MDS promoter and alternative splicing. EVI and MDS/EVI have been implicated in a number of cancers including breast, leukemia, ovarian, and intestinal cancers. We show that EVI and MDS/EVI transcripts are upregulated by T3 in the epithelium but not the rest of the intestine in Xenopus laevis when adult stem cells are forming in the epithelium. Our results suggest that EVI and MDS/EVI are likely involved in the development and/or proliferation of newly forming adult intestinal epithelial cells.
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23383234
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Figure 2. Tissue specific upregulation of EVI, MDS, and MDS/EVI transcripts in the intestine during metamorphosis.The expression of the transcripts was analyzed by using transcript-specific primer sets with qPCR on total RNA from the isolated intestinal epithelium (Ep) or the rest of the intestinal tissues (Non-Ep) of tadpoles at prometamorphosis (stage 56), the climax of metamorphosis (stage 61), and the end of metamorphosis (stage 66). The relative expression was obtained after normalizing against the control gene EF1α and was presented with the level at Ep 56 set to 1, allowing for easy comparison within each transcript. However, these data do not allow for comparisons of the expression levels between different transcript variants. Error bars represent the SEM (n = 3). * indicates transcript levels lower than the peak expression of the specific transcript in the indicated tissue (p≤0.05).
Figure 3. EVI, MDS, and MDS/EVI transcripts are induced in the intestine of premetamorphic tadpoles treated with T3.The expression of the transcripts was analyzed by using transcript-specific primer sets (as in Fig. 2.) on total intestinal RNA from tadpoles at stage 54 exposed to 5 nM T3 for 0–7 days. Error bars indicate SEM (n = 3). * indicates transcript levels lower than the peak expression of the specific transcript (p≤0.05).
Figure 4. MDS/EVI is strongly upregulated in the intestine at the climax of metamorphosis.A primer set common to EVI and MDS/EVI was used for qPCR analysis on total intestinal RNA at different stages during metamorphosis as in Fig. 2. Note that the data is consistent with that in Fig. 2 if one considers the fact that there is little non-Ep in premetamorphic tadpoles at stage 56 but non-Ep increases as a percentage of the total intestine during metamorphosis. Error bars indicate SEM (n = 3). * indicates transcript levels lower than the peak expression (p≤0.05).
Amano,
Metamorphosis-associated and region-specific expression of calbindin gene in the posterior intestinal epithelium of Xenopus laevis larva.
1998, Pubmed,
Xenbase
Amano,
Metamorphosis-associated and region-specific expression of calbindin gene in the posterior intestinal epithelium of Xenopus laevis larva.
1998,
Pubmed
,
Xenbase
Bard-Chapeau,
Ecotopic viral integration site 1 (EVI1) regulates multiple cellular processes important for cancer and is a synergistic partner for FOS protein in invasive tumors.
2012,
Pubmed
Buchholz,
Pairing morphology with gene expression in thyroid hormone-induced intestinal remodeling and identification of a core set of TH-induced genes across tadpole tissues.
2007,
Pubmed
,
Xenbase
Crosnier,
Organizing cell renewal in the intestine: stem cells, signals and combinatorial control.
2006,
Pubmed
Das,
Identification of direct thyroid hormone response genes reveals the earliest gene regulation programs during frog metamorphosis.
2009,
Pubmed
,
Xenbase
Gao,
The Evi1, microRNA-143, K-Ras axis in colon cancer.
2011,
Pubmed
Harper,
The transcriptional repressor Blimp1/Prdm1 regulates postnatal reprogramming of intestinal enterocytes.
2011,
Pubmed
Hasebe,
Epithelial-connective tissue interactions induced by thyroid hormone receptor are essential for adult stem cell development in the Xenopus laevis intestine.
2011,
Pubmed
,
Xenbase
Hasebe,
Thyroid hormone-induced sonic hedgehog signal up-regulates its own pathway in a paracrine manner in the Xenopus laevis intestine during metamorphosis.
2012,
Pubmed
,
Xenbase
Heimeier,
Studies on Xenopus laevis intestine reveal biological pathways underlying vertebrate gut adaptation from embryo to adult.
2010,
Pubmed
,
Xenbase
Ishizuya-Oka,
Anteroposterior gradient of epithelial transformation during amphibian intestinal remodeling: immunohistochemical detection of intestinal fatty acid-binding protein.
1997,
Pubmed
,
Xenbase
Ishizuya-Oka,
Origin of the adult intestinal stem cells induced by thyroid hormone in Xenopus laevis.
2009,
Pubmed
,
Xenbase
Ishizuya-Oka,
Thyroid hormone-induced expression of sonic hedgehog correlates with adult epithelial development during remodeling of the Xenopus stomach and intestine.
2001,
Pubmed
,
Xenbase
Ishizuya-Oka,
Evolutionary insights into postembryonic development of adult intestinal stem cells.
2011,
Pubmed
Ishizuya-Oka,
Apoptosis and cell proliferation in the Xenopus small intestine during metamorphosis.
1996,
Pubmed
,
Xenbase
Kataoka,
Evi1 is essential for hematopoietic stem cell self-renewal, and its expression marks hematopoietic cells with long-term multilineage repopulating activity.
2011,
Pubmed
Kataoka,
Ecotropic viral integration site 1, stem cell self-renewal and leukemogenesis.
2012,
Pubmed
Kinameri,
Prdm proto-oncogene transcription factor family expression and interaction with the Notch-Hes pathway in mouse neurogenesis.
2008,
Pubmed
Kress,
Thyroid hormones and the control of cell proliferation or cell differentiation: paradox or duality?
2009,
Pubmed
Kumano,
The role of Runx1/AML1 and Evi-1 in the regulation of hematopoietic stem cells.
2010,
Pubmed
MACDONALD,
CELL PROLIFERATION AND MIGRATION IN THE STOMACH, DUODENUM, AND RECTUM OF MAN: RADIOAUTOGRAPHIC STUDIES.
1964,
Pubmed
Matsuda,
An essential and evolutionarily conserved role of protein arginine methyltransferase 1 for adult intestinal stem cells during postembryonic development.
2010,
Pubmed
,
Xenbase
Matsuura,
Histone H3K79 methyltransferase Dot1L is directly activated by thyroid hormone receptor during Xenopus metamorphosis.
2012,
Pubmed
,
Xenbase
Mead,
Evi-1 expression in Xenopus.
2005,
Pubmed
,
Xenbase
Muncan,
Blimp1 regulates the transition of neonatal to adult intestinal epithelium.
2011,
Pubmed
Nanjundan,
Amplification of MDS1/EVI1 and EVI1, located in the 3q26.2 amplicon, is associated with favorable patient prognosis in ovarian cancer.
2007,
Pubmed
Patel,
Control of EVI-1 oncogene expression in metastatic breast cancer cells through microRNA miR-22.
2011,
Pubmed
Schreiber,
Remodeling of the intestine during metamorphosis of Xenopus laevis.
2005,
Pubmed
,
Xenbase
Shi,
The development of the adult intestinal stem cells: Insights from studies on thyroid hormone-dependent amphibian metamorphosis.
2011,
Pubmed
,
Xenbase
Shi,
Biphasic intestinal development in amphibians: embryogenesis and remodeling during metamorphosis.
1996,
Pubmed
,
Xenbase
Shi,
Dual functions of thyroid hormone receptors in vertebrate development: the roles of histone-modifying cofactor complexes.
2009,
Pubmed
,
Xenbase
Shi,
Thyroid hormone receptor actions on transcription in amphibia: The roles of histone modification and chromatin disruption.
2012,
Pubmed
Sterling,
Cytological and morphological analyses reveal distinct features of intestinal development during Xenopus tropicalis metamorphosis.
2012,
Pubmed
,
Xenbase
Stolow,
Xenopus sonic hedgehog as a potential morphogen during embryogenesis and thyroid hormone-dependent metamorphosis.
1995,
Pubmed
,
Xenbase
Sun,
Thyroid hormone regulation of adult intestinal stem cell development: mechanisms and evolutionary conservations.
2012,
Pubmed
,
Xenbase
Sun,
Spatio-temporal expression profile of stem cell-associated gene LGR5 in the intestine during thyroid hormone-dependent metamorphosis in Xenopus laevis.
2010,
Pubmed
,
Xenbase
Tata,
Gene expression during metamorphosis: an ideal model for post-embryonic development.
1993,
Pubmed
Van Campenhout,
Evi1 is specifically expressed in the distal tubule and duct of the Xenopus pronephros and plays a role in its formation.
2006,
Pubmed
,
Xenbase
Wieser,
The oncogene and developmental regulator EVI1: expression, biochemical properties, and biological functions.
2007,
Pubmed
Yoshimi,
Evi1 forms a bridge between the epigenetic machinery and signaling pathways.
2011,
Pubmed
Zhang,
PR-domain-containing Mds1-Evi1 is critical for long-term hematopoietic stem cell function.
2011,
Pubmed
van der Flier,
Stem cells, self-renewal, and differentiation in the intestinal epithelium.
2009,
Pubmed
