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XB-ART-47057
Biochem Biophys Res Commun May 31, 2013; 435 (2): 182-7.

β-Arrestin 1 mediates non-canonical Wnt pathway to regulate convergent extension movements.

Kim GH , Park EC , Lee H , Na HJ , Choi SC , Han JK .


Abstract
β-Arrestins are multifaceted proteins that play critical roles in termination of G protein-coupled receptor (GPCR) signaling by inducing its desensitization and internalization as well as in facilitation of many intracellular signaling pathways. Here, we examine using Xenopus embryos whether β-arrestin 1 might act as a mediator of β-catenin-independent Wnt (non-canonical) signaling. Xenopus β-arrestin 1 (xβarr1) is expressed in the tissues undergoing extensive cell rearrangements in early development. Gain- and loss-of-function analyses of xβarr1 revealed that it regulates convergent extension (CE) movements of mesodermal tissue with no effect on cell fate specification. In addition, rescue experiments showed that xβarr1 controls CE movements downstream of Wnt11/Fz7 signal and via activation of RhoA and JNK. In line with this, xβarr1 associated with key Wnt components including Ryk, Fz, and Dishevelled. Furthermore, we found that xβarr1 could recover CE movements inhibited by xβarr2 knockdown or its endocytosis defective mutant. Overall, these results suggest that β-arrestin 1 and 2 share interchangeable endocytic activity to regulate CE movements downstream of the non-canonical Wnt pathway.

PubMed ID: 23665017
Article link: Biochem Biophys Res Commun


Species referenced: Xenopus laevis
Genes referenced: arrb1 arrb2 cdc42 chrd.1 ctnnb1 daam1 dvl2 fzd4 fzd7 gprc6a gsc lrp5 mapk8 myc myod1 not rac1 rhoa ryk tbxt wnt11
Morpholinos: arrb1 MO1 arrb2 MO3


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