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XB-ART-47870
Dev Cell October 14, 2013; 27 (1): 103-12.
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Deuterosome-mediated centriole biogenesis.

Klos Dehring DA , Vladar EK , Werner ME , Mitchell JW , Hwang P , Mitchell BJ .


Abstract
The ability of cells to faithfully duplicate their two centrioles once per cell cycle is critical for proper mitotic progression and chromosome segregation. Multiciliated cells represent an interesting variation of centriole duplication in that these cells generate greater than 100 centrioles, which form the basal bodies of their motile cilia. This centriole amplification is proposed to require a structure termed the deuterosome, thought to be capable of promoting de novo centriole biogenesis. Here, we begin to molecularly characterize the deuterosome and identify it as a site for the localization of Cep152, Plk4, and SAS6. Additionally we identify CCDC78 as a centriole-associated and deuterosome protein that is essential for centriole amplification. Overexpression of Cep152, but not Plk4, SAS6, or CCDC78, drives overamplification of centrioles. However, in CCDC78 morphants, Cep152 fails to localize to the deuterosome and centriole biogenesis is impaired, indicating that CCDC78-mediated recruitment of Cep152 is required for deuterosome-mediated centriole biogenesis.

PubMed ID: 24075808
PMC ID: PMC3816757
Article link: Dev Cell
Grant support: [+]

Species referenced: Xenopus
Genes referenced: ccdc78 cep152 mcidas plk4
Morpholinos: ccdc78 MO1 ccdc78 MO2

References [+] :
Afzelius, A human syndrome caused by immotile cilia. 1976, Pubmed