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XB-ART-48418
Dev Dyn April 1, 2014; 243 (4): 509-26.

The Role of Sdf-1α signaling in Xenopus laevis somite morphogenesis.

Leal MA , Fickel SR , Sabillo A , Ramirez J , Vergara HM , Nave C , Saw D , Domingo CR .


Abstract
Stromal derived factor-1α (sdf-1α), a chemoattractant chemokine, plays a major role in tumor growth, angiogenesis, metastasis, and in embryogenesis. The sdf-1α signaling pathway has also been shown to be important for somite rotation in zebrafish (Hollway et al., 2007). Given the known similarities and differences between zebrafish and Xenopus laevis somitogenesis, we sought to determine whether the role of sdf-1α is conserved in Xenopus laevis. Using a morpholino approach, we demonstrate that knockdown of sdf-1α or its receptor, cxcr4, leads to a significant disruption in somite rotation and myotome alignment. We further show that depletion of sdf-1α or cxcr4 leads to the near absence of β-dystroglycan and laminin expression at the intersomitic boundaries. Finally, knockdown of sdf-1α decreases the level of activated RhoA, a small GTPase known to regulate cell shape and movement. Our results show that sdf-1α signaling regulates somite cell migration, rotation, and myotome alignment by directly or indirectly regulating dystroglycan expression and RhoA activation. These findings support the conservation of sdf-1α signaling in vertebrate somite morphogenesis; however, the precise mechanism by which this signaling pathway influences somite morphogenesis is different between the fish and the frog.

PubMed ID: 24357195
PMC ID: PMC4040348
Article link: Dev Dyn
Grant support: [+]

Species referenced: Xenopus laevis
Genes referenced: cxcl12 cxcr4 dag1 gap43 itgb1 lama1 odc1 rac1 rhoa tbx2 tubb
Antibodies: Dag1 Ab3 Itgb1 Ab1 Lama1 Ab1 Rac1 Ab3 Rhoa Ab3 Somite Ab1 Tubb Ab2
Morpholinos: cxcl12 MO2


Article Images: [+] show captions
References [+] :
Afonin, Cell behaviors associated with somite segmentation and rotation in Xenopus laevis. 2006, Pubmed, Xenbase