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XB-ART-48496
PLoS One January 1, 2014; 9 (1): e85026.
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40LoVe and Samba are involved in Xenopus neural development and functionally distinct from hnRNP AB.

Andreou M , Yan CY , Skourides PA .


Abstract
Heterogeneous nuclear ribonucleoproteins (hnRNPs) comprise a large group of modular RNA-binding proteins classified according to their conserved domains. This modular nature, coupled with a large choice of alternative splice variants generates functional diversity. Here, we investigate the biological differences between 40LoVe, its splice variant Samba and its pseudoallele hnRNP AB in neural development. Loss of function experiments lead to defects in neural development with reduction of eye size, which stem primarily from increased apoptosis and reduced proliferation in neural tissues. Despite very high homology between 40LoVe/Samba and hnRNP AB, these proteins display major differences in localization, which appear to be in part responsible for functional differences. Specifically, we show that the 40Love/Samba carboxy-terminal domain (GRD) enables nucleocytoplasmic shuttling behavior. This domain is slightly different in hnRNP AB, leading to nuclear-restricted localization. Finally, we show that shuttling is required for 40LoVe/Samba function in neural development.

PubMed ID: 24454782
PMC ID: PMC3893134
Article link: PLoS One


Species referenced: Xenopus
Genes referenced: actl6a chrd.1 hnrnpab hnrnpc mtor rax rrm1 rrm2.2 sox10 sox2 tubb2b
Antibodies: Tubb Ab1
Morpholinos: hnrnpab MO1 hnrnpab MO2


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References [+] :
Bemark, Purification and characterization of a protein binding to the SP6 kappa promoter. A potential role for CArG-box binding factor-A in kappa transcription. 1998, Pubmed