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Anat Cell Biol March 1, 2014; 47 (1): 18-27.

Expression and localization of Rdd proteins in Xenopus embryo.

Lim JC , Kurihara S , Tamaki R , Mashima Y , Maéno M .

The previous study has shown that repeated D domain-like (Rdd) proteins, a group of novel secretory proteins consisting of repeated domains of a cysteine-rich sequence, are involved in the process of blood vessel formation in Xenopus embryo. We performed further experiments to examine the localization of Rdd proteins in embryogenesis. Detection of tagged Rdd proteins expressed in blastomeres showed that Rdd proteins formed a high molecular weight complex and existed in the extracellular space. A rabbit antibody against the Rdd synthetic peptide identified a single band of 28 kD in embryonic tissue extract. By whole-mount immunostaining analysis, signal was detected in the regions of inter-somites, vitelline veins, and branchial arches at the tailbud stage. Staining of Rdd was remarkably reduced in the embryos injected with vascular endothelial growth factor Morpholino. We suggest that Rdd proteins interact with a molecule(s) associated with vascular precursor cells.

PubMed ID: 24693479
PMC ID: PMC3968263
Article link: Anat Cell Biol

Species referenced: Xenopus
Genes referenced: mif myc rdd2 rdd3 rdd4 tek val vegfa
Antibodies: Rdd Ab1
Morpholinos: rdd3 MO2 vegfa MO4

Article Images: [+] show captions
References [+] :
Carmeliet, Abnormal blood vessel development and lethality in embryos lacking a single VEGF allele. 1996, Pubmed