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J Biomed Res July 1, 2014; 28 (4): 275-81.

IRE1α knockdown rescues tunicamycin-induced developmental defects and apoptosis in Xenopus laevis.

Yuan L , Yu J , Li X , Feng J , Yin C , Wang X .

Inositol requiring enzyme-1 (IRE1) is highly conserved from yeasts to humans. Upon endoplasmic reticulum (ER) stress, IRE1 activates X-box-binding protein 1 (XBP1) by unconventional splicing of XBP1 mRNA, which activates unfolded protein response (UPR) to restore ER homeostasis. In mice, IRE1α plays an essential role in extraembryonic tissues. However, its precise action during the early stage of development is unknown. In this study, the gain and loss-of-function analyses were used to investigate the function of Xenopus IRE1α (xIRE1α). The effects of xIRE1α during embryo development were detected with RT-PCR and whole mount in situ hybridization. ER stress was induced by tunicamycin. The apoptotic cells were measured by TUNNEL assays. Although both gain and loss of xIRE1α function had no significant effect on Xenopus embryogenesis, knockdown of xIRE1α could rescue tunicamycin-induced developmental defects and apoptosis. The finding indicates that xIRE1α is not required for embryogenesis but is required for tunicamycin-induced developmental defects and apoptosis in Xenopus laevis.

PubMed ID: 25050111
PMC ID: PMC4102841
Article link: J Biomed Res

Species referenced: Xenopus laevis
Genes referenced: ddit3 ern1 sox17a tbxt xbp1
Morpholinos: ern1 MO2 xbp1 MO2

Article Images: [+] show captions
References [+] :
Acosta-Alvear, XBP1 controls diverse cell type- and condition-specific transcriptional regulatory networks. 2007, Pubmed