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XB-ART-49442
Dev Biol November 15, 2014; 395 (2): 287-98.

Chibby functions in Xenopus ciliary assembly, embryonic development, and the regulation of gene expression.

Shi J , Zhao Y , Galati D , Winey M , Klymkowsky MW .


Abstract
Wnt signaling and ciliogenesis are core features of embryonic development in a range of metazoans. Chibby (Cby), a basal-body associated protein, regulates β-catenin-mediated Wnt signaling in the mouse but not Drosophila. Here we present an analysis of Cby''s embryonic expression and morphant phenotypes in Xenopus laevis. Cby RNA is supplied maternally, negatively regulated by Snail2 but not Twist1, preferentially expressed in the neuroectoderm, and regulates β-catenin-mediated gene expression. Reducing Cby levels reduced the density of multiciliated cells, the number of basal bodies per multiciliated cell, and the numbers of neural tube primary cilia; it also led to abnormal development of the neural crest, central nervous system, and pronephros, all defects that were rescued by a Cby-GFP chimera. Reduction of Cby led to an increase in Wnt8a and decreases in Gli2, Gli3, and Shh RNA levels. Many, but not all, morphant phenotypes were significantly reversed by the Wnt inhibitor SFRP2. These observations extend our understanding of Cby''s role in mediating the network of interactions between ciliogenesis, signaling systems and tissue patterning.

PubMed ID: 25220153
PMC ID: PMC4539557
Article link: Dev Biol
Grant support: [+]

Species referenced: Xenopus laevis
Genes referenced: bmp4 cby1 cetn1 ctnnb1 ctrl dkk1 eef1a1 egr2 en2 fgf8 foxj1 gli1 gli2 gli3 mcidas myb nog odc1 ptch1 rfx2 sfrp2 shh smo snai2 sox9 tpm1 tubb2b twist1 wnt8a
Antibodies: Cby1 Ab1 Cetn1 Ab2 Kidney Ab1 Tpm1 Ab2
Morpholinos: cby1 MO1


Article Images: [+] show captions
References [+] :
Ariizumi, Isolation and differentiation of Xenopus animal cap cells. 2009, Pubmed, Xenbase