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XB-ART-49479
J Cell Biol October 13, 2014; 207 (1): 41-57.
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The calcium-dependent ribonuclease XendoU promotes ER network formation through local RNA degradation.

Schwarz DS , Blower MD .


Abstract
How cells shape and remodel organelles in response to cellular signals is a poorly understood process. Using Xenopus laevis egg extract, we found that increases in cytosolic calcium lead to the activation of an endogenous ribonuclease, XendoU. A fraction of XendoU localizes to the endoplasmic reticulum (ER) and is required for nuclear envelope assembly and ER network formation in a catalysis-dependent manner. Using a purified vesicle fusion assay, we show that XendoU functions on the surface of ER membranes to promote RNA cleavage and ribonucleoprotein (RNP) removal. Additionally, RNA removal from the surface of vesicles by RNase treatment leads to increased ER network formation. Using human tissue culture cells, we found that hEndoU localizes to the ER, where it promotes the formation of ER tubules in a catalysis-dependent manner. Together, these results demonstrate that calcium-activated removal of RNA from membranes by XendoU promotes and refines ER remodeling and the formation of tubular ER.

PubMed ID: 25287301
PMC ID: PMC4195833
Article link: J Cell Biol


Species referenced: Xenopus laevis
Genes referenced: dync1i1 dync1i2 endou endoul ktn1 rpl7a rps6 ssr1
Antibodies: Dync1i1 Ab1 Endou Ab1 Ktn1 Ab1 Rpl7a Ab1 Rps6 Ab2 Ssr1 Ab1 Tuba4b Ab2


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References [+] :
Allan, Protein phosphatase 1 regulates the cytoplasmic dynein-driven formation of endoplasmic reticulum networks in vitro. 1995, Pubmed, Xenbase