Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Proc Natl Acad Sci U S A
2003 Aug 05;10016:9602-7. doi: 10.1073/pnas.1133424100.
Show Gene links
Show Anatomy links
Two previously undescribed members of the mouse CPEB family of genes and their inducible expression in the principal cell layers of the hippocampus.
Theis M
,
Si K
,
Kandel ER
.
???displayArticle.abstract???
Cytoplasmic polyadenylation element-binding (CPEB) proteins control polyadenylation-induced translation in early development. Studies in oocytes led to the delineation of Xenopus CPEB, the first member of the family to be identified, and its mouse homologue mCPEB-1. Recently, a second mouse family member, mCPEB-2, has been described in germ cells. Increasing evidence also implicates CPEB proteins as being important in the hippocampus, where these proteins are thought to regulate local protein synthesis and synaptic plasticity. We therefore carried out a systematic screen for CPEB genes in the mouse brain and report two previously undescribed gene family members: mCPEB-3 and -4. We next examined the expression of all four genes in the hippocampus and found that mCPEB-1, -2, and -4 transcripts are expressed in the principal cell layer in the CA3 and CA1 region and in the dentate gyrus of the hippocampus. mCPEB-3 was barely expressed in naïve animals but together with mCPEB-4 was strongly up-regulated after injection of kainate to initiate seizure activity. Whereas mCPEB-1 is regulated by the Aurora kinase, mCPEB-2, -3, and -4 do not contain Aurora kinase phosphorylation sites. However, alternative splice isoforms of mCPEB-2, -3, and -4 encode the so-called B region with phosphorylation sites for cAMP-dependent protein kinase, calcium/calmodulin-dependent protein kinase II, and S6 kinase. Only isoforms that encode the B region were expressed in the principal cell layer. Coexpression of mCPEB-1 and the B region-containing splice isoforms suggests that a variety of different signaling pathways can recruit CPEB activity in hippocampal neurons.
Bliss,
Long-lasting potentiation of synaptic transmission in the dentate area of the anaesthetized rabbit following stimulation of the perforant path.
1973, Pubmed
Bliss,
Long-lasting potentiation of synaptic transmission in the dentate area of the anaesthetized rabbit following stimulation of the perforant path.
1973,
Pubmed
Casadio,
A transient, neuron-wide form of CREB-mediated long-term facilitation can be stabilized at specific synapses by local protein synthesis.
1999,
Pubmed
French,
Subfield-specific immediate early gene expression associated with hippocampal long-term potentiation in vivo.
2001,
Pubmed
Frey,
Anisomycin, an inhibitor of protein synthesis, blocks late phases of LTP phenomena in the hippocampal CA1 region in vitro.
1988,
Pubmed
Gingras,
Regulation of translation initiation by FRAP/mTOR.
2001,
Pubmed
Huang,
N-methyl-D-aspartate receptor signaling results in Aurora kinase-catalyzed CPEB phosphorylation and alpha CaMKII mRNA polyadenylation at synapses.
2002,
Pubmed
,
Xenbase
Kemp,
Protein kinase recognition sequence motifs.
1990,
Pubmed
Kennelly,
Consensus sequences as substrate specificity determinants for protein kinases and protein phosphatases.
1991,
Pubmed
Kurihara,
CPEB2, a novel putative translational regulator in mouse haploid germ cells.
2003,
Pubmed
Liu,
The cytoplasmic polyadenylation element binding protein and polyadenylation of messenger RNA in Aplysia neurons.
2003,
Pubmed
Martin,
Local protein synthesis and its role in synapse-specific plasticity.
2000,
Pubmed
Mayford,
The 3'-untranslated region of CaMKII alpha is a cis-acting signal for the localization and translation of mRNA in dendrites.
1996,
Pubmed
Mendez,
Translational control by CPEB: a means to the end.
2001,
Pubmed
,
Xenbase
Mendez,
Differential mRNA translation and meiotic progression require Cdc2-mediated CPEB destruction.
2002,
Pubmed
,
Xenbase
Mendez,
Phosphorylation of CPE binding factor by Eg2 regulates translation of c-mos mRNA.
2000,
Pubmed
,
Xenbase
Miller,
Disruption of dendritic translation of CaMKIIalpha impairs stabilization of synaptic plasticity and memory consolidation.
2002,
Pubmed
Minshall,
Dual roles of p82, the clam CPEB homolog, in cytoplasmic polyadenylation and translational masking.
1999,
Pubmed
,
Xenbase
Miyamoto,
A role of Ca2+/calmodulin-dependent protein kinase II in the induction of long-term potentiation in hippocampal CA1 area.
1996,
Pubmed
Nguyen,
Requirement of a critical period of transcription for induction of a late phase of LTP.
1994,
Pubmed
Pinna,
How do protein kinases recognize their substrates?
1996,
Pubmed
Richter,
Selective translation of mRNAs at synapses.
2002,
Pubmed
Silva,
Deficient hippocampal long-term potentiation in alpha-calcium-calmodulin kinase II mutant mice.
1992,
Pubmed
Steward,
Protein synthesis at synaptic sites on dendrites.
2001,
Pubmed
Tan,
An autoregulatory feedback loop directs the localized expression of the Drosophila CPEB protein Orb in the developing oocyte.
2001,
Pubmed
Tang,
A rapamycin-sensitive signaling pathway contributes to long-term synaptic plasticity in the hippocampus.
2002,
Pubmed
Tay,
Germ cell differentiation and synaptonemal complex formation are disrupted in CPEB knockout mice.
2001,
Pubmed
Wells,
A role for the cytoplasmic polyadenylation element in NMDA receptor-regulated mRNA translation in neurons.
2001,
Pubmed
Wu,
CPEB-mediated cytoplasmic polyadenylation and the regulation of experience-dependent translation of alpha-CaMKII mRNA at synapses.
1998,
Pubmed
,
Xenbase
Zagulska-Szymczak,
Kainate-induced genes in the hippocampus: lessons from expression patterns.
2001,
Pubmed
