Lundby A , Rossin EJ , Steffensen AB , Acha MR , Newton-Cheh C , Pfeufer A , Lynch SN , QT Interval International GWAS Consortium (QT-IGC) , Olesen SP , Brunak S , Ellinor PT , Jukema JW , Trompet S , Ford I , Macfarlane PW , Krijthe BP , Hofman A , Uitterlinden AG , Stricker BH , Nathoe HM , Spiering W , Daly MJ , Asselbergs FW , van der Harst P , Milan DJ , de Bakker PI , Lage K , Olsen JV .

K24 HL105780 NHLBI NIH HHS , P01 HD068250 NICHD NIH HHS , T32GM007753 NIGMS NIH HHS , R01 HL092577 NHLBI NIH HHS , T32 GM007753 NIGMS NIH HHS

	Figure 2. Quantitative interaction proteomics of five Mendelian LQTS proteinsa) Hierarchical cluster analysis of proteins identified in immunoprecipitation experiments visualizes the experimental specificity and reproducibility. Proteins are color-coded according to their mass-spectrometry signal intensity. Triplicates of the LQTS protein immunoprecipitations (a-c) are shown. The highlighted yellow areas indicate that each group of triplicate experiments immunoprecipitates a specific cluster of proteins. b) Volcano plots, representing the LQTS protein IPs versus IgG control IPs, show negative logarithmized t-test derived P-values (-log10(P)) as function of logarithmized ratios of average protein intensities (log2) for the LQTS protein relative to control. A hyperbolic curve indicates a false discovery rate cut-off of 0.05 and separates specific from nonspecific interactors. All points represent a protein. Purple indicates a LQTS protein, green represent proteins specifically interacting with the LQTS proteins, and blue represents nonspecific interactors.
	Figure 3. Proteomic annotation of GWAS loci coupled to experimental follow up identifies ATP1B1 as a QT variation candidate genea) Distribution of association Z-scores for genes represented in the interactomes (grey bars) to a background distribution of all genes in the genome (black line). The x-axis represents Z-scores assigned to genes corrected for SNP density and linkage disequilibrium structure. The insert shows a zoom-in of the tail of the distribution, illustrating that the distribution is significantly enriched for genes at GWS loci (P = 1.3e-6, using random sampling, see Online Methods). b) Representative current traces recorded from KCNH2 (left) and KCNH2 +ATP1B1 (right) proteins heterologously expressed in Xenopus laevis oocytes by two-electrode voltage clamp. Step currents were elicited using the depicted voltage clamp protocol with 1s pulses to test potentials ranging from −80 to +40 mV followed by deactivation (tail) current measurements at −60 mV. c) Current-voltage relationships were constructed by normalizing the steady-state currents measured at the end of each voltage step to the maximum outward current and plotting it as function of the test potential (n = 11 for KCNH2, n = 9 for KCNH2+ATP1B1). d) Channel inactivation kinetics were evaluated from currents elicited from the indicated pulse protocol. Inactivation time constants measured at +60 mV are shown for KCNH2 in absence (n = 10) or presence (n = 14) of ATP1B1. Data points are mean ± SEM. e) Cardiac action potential after Morpholino knockdown of zebrafish atp1b1a (APD80 = 256±20 msec) compared to carrier injected controls (APD80 = 321±21 msec), n = 13 independent samples per condition. * represents P<0.05. f) Superimposed normalized traces are shown for one representative sample for atp1b1a knockdown (red) and control conditions (blue).
	Figure 4. Integrative analysis of the LQTS protein network and GWAS dataa) Depiction of the interactions identified in the proteomics experiments between the LQTS proteins (purple) and proteins encoded by genes in genome-wide significant common variant loci (greene) as well as proteins encoded by genes that lie near the 28 SNPs filtered for replication genotyping (yellow). The proteins are plotted according to the best genetic association P-value of their corresponding genes in the horizontal direction after taking the negative 10 based logarithm of the P-value and in this depiction (for visualization purposes) we do not correct the P-value for multiple hypothesis testing and LD in order to preserve the true association score as determined in the GWAS. Interactions are represented by grey lines,. The dashed red line indicates the threshold for GWS (corresponding to a P-value of 5.0e-8). b) An overview of proteins in the LQTS protein network encoded by genes in all 38 loci (green) significantly assocaied to QT variation in this study and in Arking et al.4. The five proteins with yellow halos represent the three SNPs that became genome-wide significant after replication genotyping in this study (locus 1, rs7498491: EIF3C, EIF3CL, TUFM; locus 2, rs889807: SRL; locus 3, rs10824026: VCL).

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