XB-ART-49889
J Am Chem Soc
2013 Oct 23;13542:15738-15741. doi: 10.1021/ja409082w.
Show Gene links
Show Anatomy links
Evidence for phenylalanine zipper-mediated dimerization in the X-ray crystal structure of a magainin 2 analogue.
Hayouka Z
,
Mortenson DE
,
Kreitler DF
,
Weisblum B
,
Forest KT
,
Gellman SH
.
???displayArticle.abstract???
High-resolution structure elucidation has been challenging for the large group of host-defense peptides that form helices on or within membranes but do not manifest a strong folding propensity in aqueous solution. Here we report the crystal structure of an analogue of the widely studied host-defense peptide magainin 2. Magainin 2 (S8A, G13A, G18A) is a designed variant that displays enhanced antibacterial activity relative to the natural peptide. The crystal structure of magainin 2 (S8A, G13A, G18A), obtained for the racemic form, features a dimerization mode that has previously been proposed to play a role in the antibacterial activity of magainin 2 and related peptides.
???displayArticle.pubmedLink??? 24102563
???displayArticle.pmcLink??? PMC3928869
???displayArticle.link??? J Am Chem Soc
???displayArticle.grants??? [+]
R01 GM061238 NIGMS NIH HHS , R43 GM083491 NIGMS NIH HHS , T32 GM008349 NIGMS NIH HHS , T32 GM08293 NIGMS NIH HHS , T32 GM008293 NIGMS NIH HHS
Species referenced: Xenopus
Genes referenced: magainins
References [+] :
Ahmad,
Design of nontoxic analogues of cathelicidin-derived bovine antimicrobial peptide BMAP-27: the role of leucine as well as phenylalanine zipper sequences in determining its toxicity.
2009, Pubmed
Ahmad, Design of nontoxic analogues of cathelicidin-derived bovine antimicrobial peptide BMAP-27: the role of leucine as well as phenylalanine zipper sequences in determining its toxicity. 2009, Pubmed
Allende, Melittin-induced bilayer leakage depends on lipid material properties: evidence for toroidal pores. 2005, Pubmed
Avital-Shmilovici, Fully convergent chemical synthesis of ester insulin: determination of the high resolution X-ray structure by racemic protein crystallography. 2013, Pubmed
Banigan, Determination of the X-ray structure of the snake venom protein omwaprin by total chemical synthesis and racemic protein crystallography. 2010, Pubmed
Bechinger, Structure and orientation of the antibiotic peptide magainin in membranes by solid-state nuclear magnetic resonance spectroscopy. 1993, Pubmed , Xenbase
Boman, Antibacterial peptides: basic facts and emerging concepts. 2003, Pubmed
Brogden, Antimicrobial peptides: pore formers or metabolic inhibitors in bacteria? 2005, Pubmed
Bunkóczi, Crystal structures of cephaibols. 2003, Pubmed
Chen, Synthetic magainin analogues with improved antimicrobial activity. 1988, Pubmed , Xenbase
Chugh, Model for a helical bundle channel based on the high-resolution crystal structure of trichotoxin_A50E. 2002, Pubmed
Crisma, Crystal structure of a spin-labeled, channel-forming alamethicin analogue. 2007, Pubmed , Xenbase
Dang, Native chemical ligation at Asx-Cys, Glx-Cys: chemical synthesis and high-resolution X-ray structure of ShK toxin by racemic protein crystallography. 2013, Pubmed
Dhe-Paganon, A phenylalanine zipper mediates APS dimerization. 2004, Pubmed
Diamond, The roles of antimicrobial peptides in innate host defense. 2009, Pubmed
Ehrenstein, Electrically gated ionic channels in lipid bilayers. 1977, Pubmed
Epand, Dual mechanism of bacterial lethality for a cationic sequence-random copolymer that mimics host-defense antimicrobial peptides. 2008, Pubmed
Fox, A voltage-gated ion channel model inferred from the crystal structure of alamethicin at 1.5-A resolution. 1982, Pubmed
Gessmann, The crystal structure of samarosporin I at atomic resolution. 2012, Pubmed
Gregory, Magainin 2 revisited: a test of the quantitative model for the all-or-none permeabilization of phospholipid vesicles. 2009, Pubmed , Xenbase
Hancock, Cationic peptides: effectors in innate immunity and novel antimicrobials. 2001, Pubmed
Hicks, Comparison of the conformation and electrostatic surface properties of magainin peptides bound to sodium dodecyl sulfate and dodecylphosphocholine micelles. 2003, Pubmed , Xenbase
Hilpert, Sequence requirements and an optimization strategy for short antimicrobial peptides. 2006, Pubmed
Javadpour, Self-assembly of designed antimicrobial peptides in solution and micelles. 1997, Pubmed
Karle, Crystal structure of [Leu1]zervamicin, a membrane ion-channel peptide: implications for gating mechanisms. 1991, Pubmed
Karle, Conformation of the flexible bent helix of Leu1-zervamicin in crystal C and a possible gating action for ion passage. 1994, Pubmed
Karle, Crystal structure of the channel-forming polypeptide antiamoebin in a membrane-mimetic environment. 1998, Pubmed
Karle, Conformation of a 16-residue zervamicin IIA analog peptide containing three different structural features: 3(10)-helix, alpha-helix, and beta-bend ribbon. 1987, Pubmed
Krauson, Determining the mechanism of membrane permeabilizing peptides: identification of potent, equilibrium pore-formers. 2012, Pubmed
Kronen, Crystal structure and conformational analysis of ampullosporin A. 2003, Pubmed
Kumar, Molten-globule like partially folded states of human serum albumin induced by fluoro and alkyl alcohols at low pH. 2004, Pubmed
Li, NMR studies of aurein 1.2 analogs. 2006, Pubmed
Liu, Conformational transition between four and five-stranded phenylalanine zippers determined by a local packing interaction. 2006, Pubmed
Mandal, X-ray structure of native scorpion toxin BmBKTx1 by racemic protein crystallography using direct methods. 2009, Pubmed
Mandal, Racemic crystallography of synthetic protein enantiomers used to determine the X-ray structure of plectasin by direct methods. 2009, Pubmed
Mandal, Chemical synthesis and X-ray structure of a heterochiral {D-protein antagonist plus vascular endothelial growth factor} protein complex by racemic crystallography. 2012, Pubmed
Matsuzaki, Orientational and aggregational states of magainin 2 in phospholipid bilayers. 1994, Pubmed , Xenbase
Matsuzaki, An antimicrobial peptide, magainin 2, induced rapid flip-flop of phospholipids coupled with pore formation and peptide translocation. 1996, Pubmed , Xenbase
Matsuzaki, Relationship of membrane curvature to the formation of pores by magainin 2. 1998, Pubmed , Xenbase
Mishra, Ab initio design of potent anti-MRSA peptides based on database filtering technology. 2012, Pubmed
Mortenson, Quasiracemic crystallization as a tool to assess the accommodation of noncanonical residues in nativelike protein conformations. 2012, Pubmed
Mortenson, Evidence for small-molecule-mediated loop stabilization in the structure of the isolated Pin1 WW domain. 2013, Pubmed
Nicas, Characterization of vancomycin resistance in Enterococcus faecium and Enterococcus faecalis. 1989, Pubmed
Pentelute, X-ray structure of snow flea antifreeze protein determined by racemic crystallization of synthetic protein enantiomers. 2008, Pubmed
Pentelute, Total chemical synthesis and X-ray structure of kaliotoxin by racemic protein crystallography. 2010, Pubmed
Pouny, Interaction of antimicrobial dermaseptin and its fluorescently labeled analogues with phospholipid membranes. 1992, Pubmed
Rathinakumar, Broad-spectrum antimicrobial peptides by rational combinatorial design and high-throughput screening: the importance of interfacial activity. 2009, Pubmed
Sawaya, Single-wavelength phasing strategy for quasi-racemic protein crystal diffraction data. 2012, Pubmed
Sengupta, Toroidal pores formed by antimicrobial peptides show significant disorder. 2008, Pubmed
Shai, Mechanism of the binding, insertion and destabilization of phospholipid bilayer membranes by alpha-helical antimicrobial and cell non-selective membrane-lytic peptides. 1999, Pubmed , Xenbase
Snook, The structure and function of antiamoebin I, a proline-rich membrane-active polypeptide. 1998, Pubmed
Song, Crystal structure and functional mechanism of a human antimicrobial membrane channel. 2013, Pubmed
Terwilliger, The structure of melittin. II. Interpretation of the structure. 1982, Pubmed
Toniolo, Structure determination of racemic trichogin A IV using centrosymmetric crystals. 1994, Pubmed
Tossi, Amphipathic, alpha-helical antimicrobial peptides. 2000, Pubmed
Wade, All-D amino acid-containing channel-forming antibiotic peptides. 1990, Pubmed , Xenbase
Wakamatsu, Dimer structure of magainin 2 bound to phospholipid vesicles. 2002, Pubmed , Xenbase
Walshaw, Socket: a program for identifying and analysing coiled-coil motifs within protein structures. 2001, Pubmed
Wang, Structure, dynamics and mapping of membrane-binding residues of micelle-bound antimicrobial peptides by natural abundance (13)C NMR spectroscopy. 2010, Pubmed
Wang, APD: the Antimicrobial Peptide Database. 2004, Pubmed
Weisblum, Erythromycin-inducible resistance in Staphylococcus aureus: survey of antibiotic classes involved. 1969, Pubmed
Wimley, Describing the mechanism of antimicrobial peptide action with the interfacial activity model. 2010, Pubmed
Woolfson, The design of coiled-coil structures and assemblies. 2005, Pubmed
Wukovitz, Why protein crystals favour some space-groups over others. 1995, Pubmed
Yang, Barrel-stave model or toroidal model? A case study on melittin pores. 2001, Pubmed
Yanisch-Perron, Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. 1985, Pubmed
Yeaman, Mechanisms of antimicrobial peptide action and resistance. 2003, Pubmed
Yoder, Selective protein-protein interactions driven by a phenylalanine interface. 2006, Pubmed
Young, Chromosomal location of genes regulating resistance to bacteriophage in Bacillus subtilis. 1969, Pubmed
Zasloff, Antimicrobial peptides, innate immunity, and the normally sterile urinary tract. 2007, Pubmed
Zasloff, Antimicrobial peptides of multicellular organisms. 2002, Pubmed
Zawadzke, The structure of a centrosymmetric protein crystal. 1993, Pubmed