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Martinez JM
,
Wang HZ
,
Lin RZ
,
Brink PR
,
White TW
.
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Gap junction channels can modify their activity in response to cell signaling pathways. Here, we demonstrate that Connexin50 (Cx50) coupling, but not Connexin46 (Cx46), increased when co-expressed with a constitutively active p110α subunit of PI3K in Xenopus oocytes. In addition, inhibition of PI3K signaling by blocking p110α, or Akt, significantly decreased gap junctional conductance in Cx50 transfected HeLa cells, with no effect on Cx46. Alterations in coupling levels were not a result of reduced Cx50 unitary conductance, suggesting that changes in the number of active channels were responsible. These data indicate that Cx50 is specifically regulated by the PI3K signaling pathway.
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25935417
???displayArticle.pmcLink???PMC4433579 ???displayArticle.link???FEBS Lett ???displayArticle.grants???[+]
Berthoud,
Loss of function and impaired degradation of a cataract-associated mutant connexin50.
2003, Pubmed
Berthoud,
Loss of function and impaired degradation of a cataract-associated mutant connexin50.
2003,
Pubmed
Berthoud,
Roles and regulation of lens epithelial cell connexins.
2014,
Pubmed
Beyer,
Antisera directed against connexin43 peptides react with a 43-kD protein localized to gap junctions in myocardium and other tissues.
1989,
Pubmed
Boswell,
Upregulation and maintenance of gap junctional communication in lens cells.
2009,
Pubmed
Bruzzone,
Connections with connexins: the molecular basis of direct intercellular signaling.
1996,
Pubmed
Chai,
Cx50 requires an intact PDZ-binding motif and ZO-1 for the formation of functional intercellular channels.
2011,
Pubmed
Dahm,
Gap junctions containing alpha8-connexin (MP70) in the adult mammalian lens epithelium suggests a re-evaluation of its role in the lens.
1999,
Pubmed
Donaldson,
Molecular solutions to mammalian lens transparency.
2001,
Pubmed
Dunn,
Activation of Akt, not connexin 43 protein ubiquitination, regulates gap junction stability.
2012,
Pubmed
Dunn,
Injury-triggered Akt phosphorylation of Cx43: a ZO-1-driven molecular switch that regulates gap junction size.
2014,
Pubmed
Ebihara,
Expression of gap junctional proteins in Xenopus oocyte pairs.
1992,
Pubmed
,
Xenbase
Evans,
Gap junctions: structure and function (Review).
2002,
Pubmed
Fleishman,
A Calpha model for the transmembrane alpha helices of gap junction intercellular channels.
2004,
Pubmed
Goetz,
Exploring mechanisms of FGF signalling through the lens of structural biology.
2013,
Pubmed
Gong,
Disruption of alpha3 connexin gene leads to proteolysis and cataractogenesis in mice.
1997,
Pubmed
Goodenough,
Lens metabolic cooperation: a study of mouse lens transport and permeability visualized with freeze-substitution autoradiography and electron microscopy.
1980,
Pubmed
Goodenough,
The crystalline lens. A system networked by gap junctional intercellular communication.
1992,
Pubmed
Harris,
Emerging issues of connexin channels: biophysics fills the gap.
2001,
Pubmed
Lampe,
The effects of connexin phosphorylation on gap junctional communication.
2004,
Pubmed
Le,
A novel role for FGF and extracellular signal-regulated kinase in gap junction-mediated intercellular communication in the lens.
2001,
Pubmed
Lovicu,
Growth factor regulation of lens development.
2005,
Pubmed
Lu,
Increased persistent sodium current due to decreased PI3K signaling contributes to QT prolongation in the diabetic heart.
2013,
Pubmed
Martinez,
Conditional mutations of beta-catenin and APC reveal roles for canonical Wnt signaling in lens differentiation.
2009,
Pubmed
Mathias,
Physiological properties of the normal lens.
1997,
Pubmed
Mathias,
Lens gap junctions in growth, differentiation, and homeostasis.
2010,
Pubmed
Paul,
Connexin46, a novel lens gap junction protein, induces voltage-gated currents in nonjunctional plasma membrane of Xenopus oocytes.
1991,
Pubmed
,
Xenbase
Raju,
A Network Map of FGF-1/FGFR Signaling System.
2014,
Pubmed
Robinson,
An essential role for FGF receptor signaling in lens development.
2006,
Pubmed
Rong,
Disruption of Gja8 (alpha8 connexin) in mice leads to microphthalmia associated with retardation of lens growth and lens fiber maturation.
2002,
Pubmed
Sellitto,
Connexin50 is essential for normal postnatal lens cell proliferation.
2004,
Pubmed
Sellitto,
AKT activation promotes PTEN hamartoma tumor syndrome-associated cataract development.
2013,
Pubmed
Shakespeare,
Interaction between Connexin50 and mitogen-activated protein kinase signaling in lens homeostasis.
2009,
Pubmed
,
Xenbase
Shearer,
Posttranslational modifications in lens fiber connexins identified by off-line-HPLC MALDI-quadrupole time-of-flight mass spectrometry.
2008,
Pubmed
Solan,
Connexin43 phosphorylation: structural changes and biological effects.
2009,
Pubmed
Spray,
Equilibrium properties of a voltage-dependent junctional conductance.
1981,
Pubmed
,
Xenbase
Srinivas,
Voltage dependence of macroscopic and unitary currents of gap junction channels formed by mouse connexin50 expressed in rat neuroblastoma cells.
1999,
Pubmed
Wang,
MAPK/ERK1/2 and PI3-kinase signalling pathways are required for vitreous-induced lens fibre cell differentiation.
2009,
Pubmed
Wang,
Growth factor signaling in vitreous humor-induced lens fiber differentiation.
2010,
Pubmed
Weber,
Phosphatidylinositol 3-kinase is necessary for lens fiber cell differentiation and survival.
2006,
Pubmed
Wei,
Connexins and cell signaling in development and disease.
2004,
Pubmed
White,
Targeted ablation of connexin50 in mice results in microphthalmia and zonular pulverulent cataracts.
1998,
Pubmed
White,
Mouse Cx50, a functional member of the connexin family of gap junction proteins, is the lens fiber protein MP70.
1992,
Pubmed
,
Xenbase
White,
Optimal lens epithelial cell proliferation is dependent on the connexin isoform providing gap junctional coupling.
2007,
Pubmed
White,
Unique and redundant connexin contributions to lens development.
2002,
Pubmed
