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Chemistry June 8, 2015; 21 (24):

Frontispiece: Labeling Strategy and Signal Broadening Mechanism of Protein NMR Spectroscopy in Xenopus laevis Oocytes.

Ye Y , Liu X , Chen Y , Xu G , Wu Q , Zhang Z , Yao C , Liu M , Li C .

NMR Spectroscopy In their Communication on page 8686 ff., C. Li et al., demonstrate that (19) F labeling is a good first choice for studying globular and disordered proteins in Xenopus oocytes, especially compared with conventional (15) N- or (13) C-methyl enrichment. By using (19) F labeling, they found that, unlike E. coli cells, the viscosity in oocytes is only about 1.2 times that of water and that inhomogeneous broadening contributes 60-70 % to the line width. The labeling strategies and resonance broadening mechanisms in Xenopus oocytes were explored with the goal of expanding the application of this cell type.

PubMed ID: 26017161
Article link: Chemistry