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Xenopus CAF1 requires NOT1-mediated interaction with 4E-T to repress translation in vivo.
Waghray S
,
Williams C
,
Coon JJ
,
Wickens M
.
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RNA-regulatory factors bound to 3' UTRs control translation and stability. Repression often is associated with poly(A) removal. The deadenylase CAF1 is a core component of the CCR4-NOT complex. Our prior studies established that CAF1 represses translation independent of deadenylation. We sought the mechanism of its deadenylation-independent repression in Xenopus oocytes. Our data reveal a chain of interacting proteins that links CAF1 to CCR4-NOT and to Xp54 and 4E-T. Association of CAF1 with NOT1, the major subunit of CCR4-NOT, is required for repression by CAF1 tethered to a reporter mRNA. Affinity purification-mass spectrometry and coimmunoprecipitation revealed that at least five members of the CCR4-NOT complex were recruited by CAF1. The recruitment of these proteins required NOT1, as did the ability of tethered CAF1 to repress translation. In turn, NOT1 was needed to recruit Xp54 and 4E-T. We examined the role of 4E-T in repression using mutations that disrupted either eIF4E-dependent or -independent mechanisms. Expression of a 4E-T truncation that still bound eIF4E alleviated repression by tethered CAF1, NOT1, and Xp54. In contrast, a mutant 4E-T that failed to bind eIF4E did not. Repression of global translation was affected only by the eIF4E-dependent mechanism. Reporters bearing IRES elements revealed that repression via tethered CAF1 and Xp54 is cap- and eIF4E-independent, but requires one or more of eIF4A, eIF4B, and eIF4G. We propose that RNA-binding proteins, and perhaps miRNAs, repress translation through an analogous chain of interactions that begin with the 3' UTR-bound repressor and end with the noncanonical activity of 4E-T.
Aslam,
The Ccr4-NOT deadenylase subunits CNOT7 and CNOT8 have overlapping roles and modulate cell proliferation.
2009, Pubmed
Aslam,
The Ccr4-NOT deadenylase subunits CNOT7 and CNOT8 have overlapping roles and modulate cell proliferation.
2009,
Pubmed
Bai,
The CCR4 and CAF1 proteins of the CCR4-NOT complex are physically and functionally separated from NOT2, NOT4, and NOT5.
1999,
Pubmed
Basquin,
Architecture of the nuclease module of the yeast Ccr4-not complex: the Not1-Caf1-Ccr4 interaction.
2012,
Pubmed
Bawankar,
NOT10 and C2orf29/NOT11 form a conserved module of the CCR4-NOT complex that docks onto the NOT1 N-terminal domain.
2013,
Pubmed
Bhandari,
Structural basis for the Nanos-mediated recruitment of the CCR4-NOT complex and translational repression.
2014,
Pubmed
Bianchin,
Conservation of the deadenylase activity of proteins of the Caf1 family in human.
2005,
Pubmed
Braun,
GW182 proteins directly recruit cytoplasmic deadenylase complexes to miRNA targets.
2011,
Pubmed
Chekulaeva,
miRNA repression involves GW182-mediated recruitment of CCR4-NOT through conserved W-containing motifs.
2011,
Pubmed
Chen,
Mechanisms of deadenylation-dependent decay.
2011,
Pubmed
Chen,
A DDX6-CNOT1 complex and W-binding pockets in CNOT9 reveal direct links between miRNA target recognition and silencing.
2014,
Pubmed
Collart,
The Ccr4--not complex.
2012,
Pubmed
Coller,
The DEAD box helicase, Dhh1p, functions in mRNA decapping and interacts with both the decapping and deadenylase complexes.
2001,
Pubmed
Cooke,
Translational repression by deadenylases.
2010,
Pubmed
,
Xenbase
Cox,
Accurate proteome-wide label-free quantification by delayed normalization and maximal peptide ratio extraction, termed MaxLFQ.
2014,
Pubmed
Cox,
Andromeda: a peptide search engine integrated into the MaxQuant environment.
2011,
Pubmed
Cox,
MaxQuant enables high peptide identification rates, individualized p.p.b.-range mass accuracies and proteome-wide protein quantification.
2008,
Pubmed
Dickson,
Poly(A) polymerase and the regulation of cytoplasmic polyadenylation.
2001,
Pubmed
,
Xenbase
Doidge,
Deadenylation of cytoplasmic mRNA by the mammalian Ccr4-Not complex.
2012,
Pubmed
Fabian,
miRNA-mediated deadenylation is orchestrated by GW182 through two conserved motifs that interact with CCR4-NOT.
2011,
Pubmed
Fabian,
Mammalian miRNA RISC recruits CAF1 and PABP to affect PABP-dependent deadenylation.
2009,
Pubmed
Fabian,
Structural basis for the recruitment of the human CCR4-NOT deadenylase complex by tristetraprolin.
2013,
Pubmed
Ferraiuolo,
A role for the eIF4E-binding protein 4E-T in P-body formation and mRNA decay.
2005,
Pubmed
Fischer,
The DEAD box protein Dhh1 stimulates the decapping enzyme Dcp1.
2002,
Pubmed
Garneau,
The highways and byways of mRNA decay.
2007,
Pubmed
Gebauer,
Molecular mechanisms of translational control.
2004,
Pubmed
Goldstrohm,
PUF proteins bind Pop2p to regulate messenger RNAs.
2006,
Pubmed
Goldstrohm,
Multifunctional deadenylase complexes diversify mRNA control.
2008,
Pubmed
Gray,
Multiple portions of poly(A)-binding protein stimulate translation in vivo.
2000,
Pubmed
,
Xenbase
Horiuchi,
Structural basis for the antiproliferative activity of the Tob-hCaf1 complex.
2009,
Pubmed
Hosoda,
Anti-proliferative protein Tob negatively regulates CPEB3 target by recruiting Caf1 deadenylase.
2011,
Pubmed
Inada,
Novel roles of the multi-functional CCR4-NOT complex in post-transcriptional regulation.
2014,
Pubmed
Kadyrova,
Translational control of maternal Cyclin B mRNA by Nanos in the Drosophila germline.
2007,
Pubmed
Kamenska,
Human 4E-T represses translation of bound mRNAs and enhances microRNA-mediated silencing.
2014,
Pubmed
Kieft,
Viral IRES RNA structures and ribosome interactions.
2008,
Pubmed
Kwak,
Mammalian GLD-2 homologs are poly(A) polymerases.
2004,
Pubmed
,
Xenbase
Lau,
Human Ccr4-Not complexes contain variable deadenylase subunits.
2009,
Pubmed
Lund,
Limiting Ago protein restricts RNAi and microRNA biogenesis during early development in Xenopus laevis.
2011,
Pubmed
,
Xenbase
Mathys,
Structural and biochemical insights to the role of the CCR4-NOT complex and DDX6 ATPase in microRNA repression.
2014,
Pubmed
Meijer,
Translational repression and eIF4A2 activity are critical for microRNA-mediated gene regulation.
2013,
Pubmed
Miller,
Ccr4-Not complex: the control freak of eukaryotic cells.
2012,
Pubmed
Minshall,
The active form of Xp54 RNA helicase in translational repression is an RNA-mediated oligomer.
2004,
Pubmed
,
Xenbase
Minshall,
CPEB interacts with an ovary-specific eIF4E and 4E-T in early Xenopus oocytes.
2007,
Pubmed
,
Xenbase
Molin,
C. elegans homologue of the Caf1 gene, which encodes a subunit of the CCR4-NOT complex, is essential for embryonic and larval development and for meiotic progression.
2005,
Pubmed
Nasertorabi,
Insights into the structure of the CCR4-NOT complex by electron microscopy.
2011,
Pubmed
Nesvizhskii,
Interpretation of shotgun proteomic data: the protein inference problem.
2005,
Pubmed
Pestova,
Canonical eukaryotic initiation factors determine initiation of translation by internal ribosomal entry.
1996,
Pubmed
Petit,
The structural basis for the interaction between the CAF1 nuclease and the NOT1 scaffold of the human CCR4-NOT deadenylase complex.
2012,
Pubmed
Plank,
The structures of nonprotein-coding RNAs that drive internal ribosome entry site function.
2012,
Pubmed
Rouya,
Human DDX6 effects miRNA-mediated gene silencing via direct binding to CNOT1.
2014,
Pubmed
Sandler,
Not1 mediates recruitment of the deadenylase Caf1 to mRNAs targeted for degradation by tristetraprolin.
2011,
Pubmed
Suzuki,
Interaction of NANOS2 and NANOS3 with different components of the CNOT complex may contribute to the functional differences in mouse male germ cells.
2014,
Pubmed
Suzuki,
NANOS2 interacts with the CCR4-NOT deadenylation complex and leads to suppression of specific RNAs.
2010,
Pubmed
Temme,
Deadenylation of mRNA by the CCR4-NOT complex in Drosophila: molecular and developmental aspects.
2014,
Pubmed
Wahle,
RNA decay machines: deadenylation by the Ccr4-not and Pan2-Pan3 complexes.
2013,
Pubmed
Wilusz,
The cap-to-tail guide to mRNA turnover.
2001,
Pubmed
Xu,
Insights into the structure and architecture of the CCR4-NOT complex.
2014,
Pubmed
Yamashita,
Concerted action of poly(A) nucleases and decapping enzyme in mammalian mRNA turnover.
2005,
Pubmed
