Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
A comprehensive search for calcium binding sites critical for TMEM16A calcium-activated chloride channel activity.
Tien J
,
Peters CJ
,
Wong XM
,
Cheng T
,
Jan YN
,
Jan LY
,
Yang H
.
???displayArticle.abstract??? TMEM16A forms calcium-activated chloride channels (CaCCs) that regulate physiological processes such as the secretions of airway epithelia and exocrine glands, the contraction of smooth muscles, and the excitability of neurons. Notwithstanding intense interest in the mechanism behind TMEM16A-CaCC calcium-dependent gating, comprehensive surveys to identify and characterize potential calcium sensors of this channel are still lacking. By aligning distantly related calcium-activated ion channels in the TMEM16 family and conducting systematic mutagenesis of all conserved acidic residues thought to be exposed to the cytoplasm, we identify four acidic amino acids as putative calcium-binding residues. Alterations of the charge, polarity, and size of amino acid side chains at these sites alter the ability of different divalent cations to activate the channel. Furthermore, TMEM16A mutant channels containing double cysteine substitutions at these residues are sensitive to the redox potential of the internal solution, providing evidence for their physical proximity and solvent accessibility.
Figure 1. Calmodulin (CaM) is not responsible for the calcium-dependent activation of TMEM16A calcium-activated chloride channels (CaCC).(A) Two competing models to explain TMEM16A calcium sensitivity have been proposed. It is unclear whether calcium directly binds to TMEM16A-CaCCs (upper panel) or whether CaM is required to mediate the calcium sensitivity of the channel (lower panel). (B) Representative current traces of wildtype mouse TMEM16A-CaCC (mTMEM16A) recorded at +60 mV and −60 mV in response to various intracellular calcium concentrations using an inside-out patch clamp configuration. Table indicates the concentration of calcium used. (C) Calcium dose–response of mTMEM16A channel at +60 mV and −60 mV. The smooth curves represent fits to the Hill equation (‘Materials and methods’). (D) Loss-of-function CaM mutants (CaM12, CaM34, CaM1234) did not reduce the apparent calcium sensitivity of the endogenous TMEM16A (x16A) channel in Xenopus oocytes. n.s.: non-significant. (E) Application of monoclonal anti-CaM antibody CaM85 (2 μg/ml) to the cytosolic face of inside-out patches had no effect on the calcium sensitivity of endogenous Xenopus TMEM16A-CaCC. (F) Mutating residues reported by Vocke et al. (2013) to be in the CaM binding domain of mTMEM16A did not affect apparent TMEM16A-CaCC calcium sensitivity.DOI:
http://dx.doi.org/10.7554/eLife.02772.003Figure 1—figure supplement 1. Calmodulin (CaM) is not involved in the calcium-dependent activation of TMEM16A-CaCC.(A) Acute application of 50 µM W7, a CaM antagonist, to the cytosolic face of the inside-out patches failed to inhibit endogenous Xenopus TMEM16A-CaCC currents. (B) Chronic incubation of Xenopus oocytes with W7 did not reduce the apparent calcium sensitivity of endogenous Xenopus TMEM16A-CaCC. (C and D) Barium, a divalent cation that is incapable of binding CaM, can robustly activate mouse TMEM16A-CaCC. (C) CaCC currents were recorded with voltage steps in +20 mV increments from −80 mV to +120 mV in isotonic 140 mM NaCl solutions. Both holding and repolarizing potentials were −80 mV. Dotted lines indicate the zero current level. (D) Barium dose–response curves of wildtype mTMEM16A channel at +60 mV and −60 mV. Smooth curve represent fits to the Hill equation.DOI:
http://dx.doi.org/10.7554/eLife.02772.004
Figure 2. Screen for potential calcium-binding residues in TMEM16A-CaCC.(A and B) Quantification of the apparent calcium sensitivity of E698Q and E701Q (Yu et al., 2012) mutant TMEM16A channels. (A) Representative current traces of E698Q and E701Q mutants in response to intracellular solutions with different calcium concentrations recorded at +60 mV. Table indicates the concentration of calcium used. (B) Calcium dose–response curves of the mTMEM16A channels at +60 mV. Smooth curves represent fits to the Hill equation. (C) Sequence alignment of the calcium-activated TMEM16 channels. h16A, m16A, x16A, m16B, m16F and Fly16 are the human TMEM16A (Uniprot ID #Q5XXA6), mouse TMEM16A (Uniprot ID #Q8BHY3-2), Xenopus TMEM16A (Uniprot ID #B5SVV6), mouse TMEM16B (Uniprot ID #Q8CFW1), mouse TMEM16F (Uniprot ID #Q6P9J9) and Drosophila TMEM16 channels (Uniprot ID #Q86P24), respectively. Highly conserved acidic residues that are potentially exposed to the cytoplasm are highlighted in red. Some residues with conserved oxygen-containing side chains in m16F and Fly16 are highlighted in green. Putative transmembrane (TM) segments are highlighted in cyan. The controversial TM6 segments are highlighted in gray and labeled as TM6′ and TM6″, respectively. ‘In’ and ‘Out’ indicate the intracellular and extracellular side of the membrane, respectively.DOI:
http://dx.doi.org/10.7554/eLife.02772.005
Figure 3. Systematic alanine scan of highly conserved intracellular acidic residues identified five mutations that dramatically reduced the apparent calcium sensitivity of TMEM16A-CaCC.(A) Representative current traces of the E650A, E698A, E701A, E730A and D734A mutant channels in response to different intracellular calcium solutions recorded at +60 mV. Table indicates the concentration of calcium used. (B) Calcium dose–response curves of these mutant mTMEM16A channels at +60 mV. Smooth curves represent fits to the Hill equation. (C) Summary of apparent calcium sensitivity (EC50s) of all alanine mutants tested. Dotted line indicates a 10-fold increase in EC50 compared to wildtype channels.DOI:
http://dx.doi.org/10.7554/eLife.02772.006
Figure 4. The effects of different amino acid side chains on the calcium sensitivity of mutant TMEM16A-CaCC channels indicate that E698, E701, E730 and D734 might be directly involved in binding calcium.(A–E) Calcium dose–response curves of (A) E650, (B) E698, (C) E701, (D), E730, and (E) D734 mutant mTMEM16A channels at +60 mV. Smooth curves represent fits to the Hill equation. Maximum activity was constrained to 1 for these fittings. (F) Summary of apparent calcium sensitivity (EC50s) of mTMEM16A mutants. N.C.: no obvious CaCC current recorded. ***p<0.001.DOI:
http://dx.doi.org/10.7554/eLife.02772.008
Figure 5. TMEM16A channel sensitivity to strontium ions is disrupted by mutations of the identified calcium-binding sites.(A) Representative current trace of wildtype mTMEM16A in response to different intracellular strontium solutions recorded at +60 mV. Table indicates the concentration of strontium used. (B) Strontium dose–response curve of wildtype mTMEM16A channels at +60 mV. (C–G) Strontium dose–response curves of the (C) E650, (D) E698, (E) E701, (F) E730, and (G) D734 mutant mTMEM16A channels at +60 mV. Smooth curves represent fits to the Hill equation. (H) Summary of apparent strontium sensitivity (EC50s) of mTMEM16A mutants. N.C.: no obvious CaCC current recorded. Upward arrows: estimated strontium EC50 >10 mM and cannot be reported with confidence. ***p<0.001.DOI:
http://dx.doi.org/10.7554/eLife.02772.010
Figure 6. TMEM16A channel sensitivity to cadmium ions is disrupted by mutations at the identified calcium-binding sites.(A) Representative current trace of wildtype mTMEM16A in response to different intracellular cadmium solutions recorded at +60 mV. Table indicates the concentration of cadmium used. (B) Cadmium dose–response curve of wildtype mTMEM16A channels at +60 mV. (C–G) Cadmium dose–response curves of the (C) E650, (D) E698, (E) E701, (F) E730, and (G) D734 mutant mTMEM16A channels at +60 mV. Smooth curves represent fits to the Hill equation. (H) Summary of apparent cadmium sensitivity (EC50s) of mTMEM16A mutants. Upward arrows: estimated cadmium EC50 >10 mM and cannot be reported with confidence. *p<0.05; **p<0.01; ***p<0.001.DOI:
http://dx.doi.org/10.7554/eLife.02772.009
Figure 7. Cysteine crosslinking suggests that the calcium-binding residues in TMEM16A-CaCC form a metal ion binding pocket that is exposed to the cytoplasm.(A) Representative traces of E701C/E730C, E701C, and E730C mTMEM16A mutants recorded under reducing (DTT) and oxidizing (H2O2) conditions. (B) Comparison of currents recorded in oxidizing conditions of mutants shown in A. When activated for long periods of time, TMEM16A-CaCCs exhibit a persistent decrease in activity, as previously described (Vocke et al., 2013). Current amplitudes were measured 60 s after the onset of perfusion and are normalized to currents recorded in reducing conditions. ***p<0.001. (C) Schematic illustrating the position of the putative calcium binding residues (E698, E701, E730 and D734) based on two previous membrane topological models (Model A and B) (Yang et al., 2008b; Yu et al., 2012) and our experimental data (Model C).DOI:
http://dx.doi.org/10.7554/eLife.02772.007
Author response image 1. Barium dose-response curve of wildtype and mutant mTMEM16A channels at +60 mV. E730D and E730Q were the only mutants that produced appreciable activity out of the channels tested.The pattern of bariumdependentactivation is not similar to that of cadmium-dependent activation.
Andersen,
Chelation of cadmium.
1984,
Pubmed
Berg,
Ca2+-activated Cl- channels at a glance.
2012,
Pubmed
,
Xenbase
Billig,
Ca2+-activated Cl− currents are dispensable for olfaction.
2011,
Pubmed
Caputo,
TMEM16A, a membrane protein associated with calcium-dependent chloride channel activity.
2008,
Pubmed
Chao,
Activation of calmodulin by various metal cations as a function of ionic radius.
1984,
Pubmed
Das,
Topology of NGEP, a prostate-specific cell:cell junction protein widely expressed in many cancers of different grade level.
2008,
Pubmed
Dudev,
Principles governing Mg, Ca, and Zn binding and selectivity in proteins.
2003,
Pubmed
Dudev,
Factors governing the metal coordination number in metal complexes from Cambridge Structural Database analyses.
2006,
Pubmed
Duvvuri,
TMEM16A induces MAPK and contributes directly to tumorigenesis and cancer progression.
2012,
Pubmed
Hartzell,
Anoctamin/TMEM16 family members are Ca2+-activated Cl- channels.
2009,
Pubmed
Huang,
Calcium-activated chloride channels (CaCCs) regulate action potential and synaptic response in hippocampal neurons.
2012,
Pubmed
Huang,
Calcium-activated chloride channel TMEM16A modulates mucin secretion and airway smooth muscle contraction.
2012,
Pubmed
Huang,
International Union of Basic and Clinical Pharmacology. LXXXV: calcium-activated chloride channels.
2012,
Pubmed
,
Xenbase
Jung,
Dynamic modulation of ANO1/TMEM16A HCO3(-) permeability by Ca2+/calmodulin.
2013,
Pubmed
Katoh,
FLJ10261 gene, located within the CCND1-EMS1 locus on human chromosome 11q13, encodes the eight-transmembrane protein homologous to C12orf3, C11orf25 and FLJ34272 gene products.
2003,
Pubmed
Kunzelmann,
Anoctamins.
2011,
Pubmed
Lishko,
The ankyrin repeats of TRPV1 bind multiple ligands and modulate channel sensitivity.
2007,
Pubmed
Maylie,
Small conductance Ca2+-activated K+ channels and calmodulin.
2004,
Pubmed
Ni,
Activation and inhibition of TMEM16A calcium-activated chloride channels.
2014,
Pubmed
Pidcock,
Structural characteristics of protein binding sites for calcium and lanthanide ions.
2001,
Pubmed
Romanenko,
Tmem16A encodes the Ca2+-activated Cl- channel in mouse submandibular salivary gland acinar cells.
2010,
Pubmed
Salkoff,
High-conductance potassium channels of the SLO family.
2006,
Pubmed
Schreiber,
A novel calcium-sensing domain in the BK channel.
1997,
Pubmed
,
Xenbase
Schroeder,
Expression cloning of TMEM16A as a calcium-activated chloride channel subunit.
2008,
Pubmed
,
Xenbase
Schumacher,
Structure of the gating domain of a Ca2+-activated K+ channel complexed with Ca2+/calmodulin.
2001,
Pubmed
Scudieri,
The anoctamin family: TMEM16A and TMEM16B as calcium-activated chloride channels.
2012,
Pubmed
Scudieri,
TMEM16A-TMEM16B chimaeras to investigate the structure-function relationship of calcium-activated chloride channels.
2013,
Pubmed
Shi,
Mechanism of magnesium activation of calcium-activated potassium channels.
2002,
Pubmed
Stöhr,
TMEM16B, a novel protein with calcium-dependent chloride channel activity, associates with a presynaptic protein complex in photoreceptor terminals.
2009,
Pubmed
Suzuki,
Calcium-dependent phospholipid scrambling by TMEM16F.
2010,
Pubmed
Sóvágó,
Cadmium(II) complexes of amino acids and peptides.
2013,
Pubmed
Terashima,
Purified TMEM16A is sufficient to form Ca2+-activated Cl- channels.
2013,
Pubmed
Tian,
Calmodulin-dependent activation of the epithelial calcium-dependent chloride channel TMEM16A.
2011,
Pubmed
Tsien,
Measurement of cytosolic free Ca2+ with quin2.
1989,
Pubmed
Vocke,
Calmodulin-dependent activation and inactivation of anoctamin calcium-gated chloride channels.
2013,
Pubmed
Wong,
Subdued, a TMEM16 family Ca²⁺-activated Cl⁻channel in Drosophila melanogaster with an unexpected role in host defense.
2013,
Pubmed
Wu,
Structure of the gating ring from the human large-conductance Ca(2+)-gated K(+) channel.
2010,
Pubmed
Xia,
Multiple regulatory sites in large-conductance calcium-activated potassium channels.
2002,
Pubmed
Xia,
Mechanism of calcium gating in small-conductance calcium-activated potassium channels.
1998,
Pubmed
,
Xenbase
Xiao,
Voltage- and calcium-dependent gating of TMEM16A/Ano1 chloride channels are physically coupled by the first intracellular loop.
2011,
Pubmed
Yang,
TMEM16F forms a Ca2+-activated cation channel required for lipid scrambling in platelets during blood coagulation.
2012,
Pubmed
,
Xenbase
Yang,
TMEM16A confers receptor-activated calcium-dependent chloride conductance.
2008,
Pubmed
,
Xenbase
Yang,
Activation of Slo1 BK channels by Mg2+ coordinated between the voltage sensor and RCK1 domains.
2008,
Pubmed
Yu,
Explaining calcium-dependent gating of anoctamin-1 chloride channels requires a revised topology.
2012,
Pubmed
Yu,
Activation of the Ano1 (TMEM16A) chloride channel by calcium is not mediated by calmodulin.
2014,
Pubmed
Yuan,
Divalent cations modulate TMEM16A calcium-activated chloride channels by a common mechanism.
2013,
Pubmed
Yuan,
Structure of the human BK channel Ca2+-activation apparatus at 3.0 A resolution.
2010,
Pubmed
Zeng,
Divalent cation sensitivity of BK channel activation supports the existence of three distinct binding sites.
2005,
Pubmed
,
Xenbase
Zhang,
Ion sensing in the RCK1 domain of BK channels.
2010,
Pubmed
,
Xenbase
Zhou,
Barium ions selectively activate BK channels via the Ca2+-bowl site.
2012,
Pubmed
,
Xenbase