Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
PLoS One
2014 Jan 01;96:e100490. doi: 10.1371/journal.pone.0100490.
Show Gene links
Show Anatomy links
Effects of cationic antimicrobial peptides on liquid-preserved boar spermatozoa.
Schulze M
,
Junkes C
,
Mueller P
,
Speck S
,
Ruediger K
,
Dathe M
,
Mueller K
.
???displayArticle.abstract???
Antibiotics are mandatory additives in semen extenders to control bacterial contamination. The worldwide increase in resistance to conventional antibiotics requires the search for alternatives not only for animal artificial insemination industries, but also for veterinary and human medicine. Cationic antimicrobial peptides are of interest as a novel class of antimicrobial additives for boar semen preservation. The present study investigated effects of two synthetic cyclic hexapeptides (c-WFW, c-WWW) and a synthetic helical magainin II amide derivative (MK5E) on boar sperm during semen storage at 16 °C for 4 days. The standard extender, Beltsville Thawing Solution (BTS) containing 250 µg/mL gentamicin (standard), was compared to combinations of BTS with each of the peptides in a split-sample procedure. Examination revealed peptide- and concentration-dependent effects on sperm integrity and motility. Negative effects were more pronounced for MK5E than in hexapeptide-supplemented samples. The cyclic hexapeptides were partly able to stimulate a linear progressive sperm movement. When using low concentrations of cyclic hexapeptides (4 µM c-WFW, 2 µM c-WWW) sperm quality was comparable to the standard extender over the course of preservation. C-WFW-supplemented boar semen resulted in normal fertility rates after AI. In order to investigate the interaction of peptides with the membrane, electron spin resonance spectroscopic measurements were performed using spin-labeled lipids. C-WWW and c-WFW reversibly immobilized an analog of phosphatidylcholine (PC), whereas MK5E caused an irreversible increase of PC mobility. These results suggest testing the antimicrobial efficiency of non-toxic concentrations of selected cyclic hexapeptides as potential candidates to supplement/replace common antibiotics in semen preservation.
???displayArticle.pubmedLink???
24940997
???displayArticle.pmcLink???PMC4062521 ???displayArticle.link???PLoS One
Figure 2. Motion parameters and percentage of damaged boar spermatozoa during BTS preservation at 16°C in the presence of 250 µg/mL gentamicin (standard), 4 µM and 8 µM c-WFW, 2 µM and 4 µM c-WWW, and 1 µM and 2 µM MK5E (n = 9).The percentage, the velocity (VAP), and the linearity (VSL/VCL) of progressive motile spermatozoa after 12 h and 96 h storage are shown (A, C, E). The percentage and the velocity (VAP) of progressive motile spermatozoa after 48 h storage are shown in a thermoresistance test (TRT, incubation at 38°C) for 30 and 300 min (B, D). The percentage of damaged spermatozoa was determined after 12 h, 48 h, and 96 h storage by staining with propidium iodide and/or markers for the acrosomal membrane and contents (F). Significant difference (see text for the exact P-values) compared to standard after 12 h (▪), 48 h (#), 96 h (▴) storage, and after TRT 30 min (○) and TRT 300 min (•) incubation at 38°C.
Figure 1. Motion parameters of boar spermatozoa after 5 min of incubation with increasing concentrations (5 µM, 10 µM, 20 µM, and 40 µM) of c-WFW, c-WWW, and MK5E (n = 12).Before the application of peptides, semen was preserved for 24°C in BTS containing 250 µg/mL gentamicin (standard). * Significant difference compared to standard (see text for the exact P-values).
Althouse,
Bacteriospermia in extended porcine semen.
2005, Pubmed
Althouse,
Bacteriospermia in extended porcine semen.
2005,
Pubmed
Appelt,
Structure of the antimicrobial, cationic hexapeptide cyclo(RRWWRF) and its analogues in solution and bound to detergent micelles.
2005,
Pubmed
Bagheri,
Interaction of W-substituted analogs of cyclo-RRRWFW with bacterial lipopolysaccharides: the role of the aromatic cluster in antimicrobial activity.
2011,
Pubmed
Broekhuijse,
Application of computer-assisted semen analysis to explain variations in pig fertility.
2012,
Pubmed
Chan,
Tryptophan- and arginine-rich antimicrobial peptides: structures and mechanisms of action.
2006,
Pubmed
Choi,
Cyclodextrin removes cholesterol from mouse sperm and induces capacitation in a protein-free medium.
1998,
Pubmed
Clara,
Preclinical evaluation of magainin-A as a contraceptive antimicrobial agent.
2004,
Pubmed
Dathe,
Cyclization increases the antimicrobial activity and selectivity of arginine- and tryptophan-containing hexapeptides.
2004,
Pubmed
Dathe,
Optimization of the antimicrobial activity of magainin peptides by modification of charge.
2001,
Pubmed
,
Xenbase
Epand,
Diversity of antimicrobial peptides and their mechanisms of action.
1999,
Pubmed
Fellmann,
Synthesis and use of spin-labeled lipids for studies of the transmembrane movement of phospholipids.
1994,
Pubmed
Hancock,
Clinical development of cationic antimicrobial peptides: from natural to novel antibiotics.
2002,
Pubmed
Junkes,
Cyclic antimicrobial R-, W-rich peptides: the role of peptide structure and E. coli outer and inner membranes in activity and the mode of action.
2011,
Pubmed
Junkes,
The interaction of arginine- and tryptophan-rich cyclic hexapeptides with Escherichia coli membranes.
2008,
Pubmed
Kurz,
Localization of phosphatidylserine in boar sperm cell membranes during capacitation and acrosome reaction.
2005,
Pubmed
Lohner,
Molecular mechanisms of membrane perturbation by antimicrobial peptides and the use of biophysical studies in the design of novel peptide antibiotics.
2005,
Pubmed
Morse,
Use of the spin label tempamine for measuring the internal viscosity of red blood cells.
1977,
Pubmed
Mortimer,
Quantitative observations of flagellar motility of capacitating human spermatozoa.
1997,
Pubmed
Müller,
Protein-dependent translocation of aminophospholipids and asymmetric transbilayer distribution of phospholipids in the plasma membrane of ram sperm cells.
1994,
Pubmed
Müller,
Characterization of sperm plasma membrane properties after cholesterol modification: consequences for cryopreservation of rainbow trout spermatozoa.
2008,
Pubmed
Müller,
The use of spin-labelled phospholipid analogues to characterize the transverse distribution of phospholipids and the activity of phospholipase-A2 in the cell membrane of bull spermatozoa.
1997,
Pubmed
Reddy,
Spermicidal activity of Magainins: in vitro and in vivo studies.
1996,
Pubmed
Rossi,
Research advances in the development of peptide antibiotics.
2008,
Pubmed
Schulze,
Development of an in vitro index to characterize fertilizing capacity of boar ejaculates.
2013,
Pubmed
Schulze,
Temperature management during semen processing: Impact on boar sperm quality under laboratory and field conditions.
2013,
Pubmed
Shadan,
Cholesterol efflux alters lipid raft stability and distribution during capacitation of boar spermatozoa.
2004,
Pubmed
Toke,
Antimicrobial peptides: new candidates in the fight against bacterial infections.
2005,
Pubmed
Waterhouse,
Within and between breed differences in freezing tolerance and plasma membrane fatty acid composition of boar sperm.
2006,
Pubmed
Wieprecht,
Peptide hydrophobicity controls the activity and selectivity of magainin 2 amide in interaction with membranes.
1997,
Pubmed
,
Xenbase
Zachowski,
Phospholipids in animal eukaryotic membranes: transverse asymmetry and movement.
1993,
Pubmed
Zhao,
LAMP: A Database Linking Antimicrobial Peptides.
2013,
Pubmed
de Waal,
Magainins affect respiratory control, membrane potential and motility of hamster spermatozoa.
1991,
Pubmed
