Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
J Biol Chem
2015 Dec 18;29051:30375-89. doi: 10.1074/jbc.M115.660506.
Show Gene links
Show Anatomy links
Hyperosmotic Shock Engages Two Positive Feedback Loops through Caspase-3-dependent Proteolysis of JNK1-2 and Bid.
Yue J
,
Ben Messaoud N
,
López JM
.
???displayArticle.abstract???
Hyperosmotic shock induces early calpain activation, Smac/DIABLO release from the mitochondria, and p38/JNK activation in Xenopus oocytes. These pathways regulate late cytochrome c release and caspase-3 activation. Here, we show that JNK1-1 and JNK1-2 are activated early by osmostress, and sustained activation of both isoforms accelerates the apoptotic program. When caspase-3 is activated, JNK1-2 is proteolyzed at Asp-385 increasing the release of cytochrome c and caspase-3 activity, thereby creating a positive feedback loop. Expression of Bcl-xL markedly reduces hyperosmotic shock-induced apoptosis. In contrast, expression of Bid induces rapid caspase-3 activation, even in the absence of osmostress, which is blocked by Bcl-xL co-expression. In these conditions a significant amount of Bid in the cytosol is mono- and bi-ubiquitinated. Caspase-3 activation by hyperosmotic shock induces proteolysis of Bid and mono-ubiquitinated Bid at Asp-52 increasing the release of cytochrome c and caspase-3 activation, and thus creating a second positive feedback loop. Revealing the JNK isoforms and the loops activated by osmostress could help to design better treatments for human diseases caused by perturbations in fluid osmolarity.
Bagowski,
c-Jun N-terminal kinase activation in Xenopus laevis eggs and embryos. A possible non-genomic role for the JNK signaling pathway.
2001, Pubmed,
Xenbase
Bagowski,
c-Jun N-terminal kinase activation in Xenopus laevis eggs and embryos. A possible non-genomic role for the JNK signaling pathway.
2001,
Pubmed
,
Xenbase
Ben Messaoud,
Osmostress-induced apoptosis in Xenopus oocytes: role of stress protein kinases, calpains and Smac/DIABLO.
2015,
Pubmed
,
Xenbase
Ben Messaoud,
Basic Properties of the p38 Signaling Pathway in Response to Hyperosmotic Shock.
2015,
Pubmed
,
Xenbase
Bhuyan,
Resting membrane potential as a marker of apoptosis: studies on Xenopus oocytes microinjected with cytochrome c.
2001,
Pubmed
,
Xenbase
Billen,
Bid: a Bax-like BH3 protein.
2008,
Pubmed
Breitschopf,
Ubiquitin-mediated degradation of the proapoptotic active form of bid. A functional consequence on apoptosis induction.
2000,
Pubmed
Davis,
Signal transduction by the JNK group of MAP kinases.
2000,
Pubmed
Degli Esposti,
Post-translational modification of Bid has differential effects on its susceptibility to cleavage by caspase 8 or caspase 3.
2003,
Pubmed
Deng,
A JNK-dependent pathway is required for TNFalpha-induced apoptosis.
2003,
Pubmed
Desagher,
Phosphorylation of bid by casein kinases I and II regulates its cleavage by caspase 8.
2001,
Pubmed
Dhanasekaran,
JNK signaling in apoptosis.
2008,
Pubmed
Enomoto,
Caspase-mediated cleavage of JNK during stress-induced apoptosis.
2003,
Pubmed
Fan,
The cleaved-Caspase-3 antibody is a marker of Caspase-9-like DRONC activity in Drosophila.
2010,
Pubmed
Kerscher,
Modification of proteins by ubiquitin and ubiquitin-like proteins.
2006,
Pubmed
Kominami,
The initiator caspase, caspase-10beta, and the BH-3-only molecule, Bid, demonstrate evolutionary conservation in Xenopus of their pro-apoptotic activities in the extrinsic and intrinsic pathways.
2006,
Pubmed
,
Xenbase
König,
Full length Bid is sufficient to induce apoptosis of cultured rat hippocampal neurons.
2007,
Pubmed
Lei,
JNK phosphorylation of Bim-related members of the Bcl2 family induces Bax-dependent apoptosis.
2003,
Pubmed
Li,
Cleavage of BID by caspase 8 mediates the mitochondrial damage in the Fas pathway of apoptosis.
1998,
Pubmed
Mandic,
Calpain-mediated Bid cleavage and calpain-independent Bak modulation: two separate pathways in cisplatin-induced apoptosis.
2002,
Pubmed
Martiáñez,
Generation of digital responses in stress sensors.
2009,
Pubmed
,
Xenbase
McDonnell,
Solution structure of the proapoptotic molecule BID: a structural basis for apoptotic agonists and antagonists.
1999,
Pubmed
McGrew,
Poly(A) elongation during Xenopus oocyte maturation is required for translational recruitment and is mediated by a short sequence element.
1989,
Pubmed
,
Xenbase
Pörn-Ares,
Cleavage of the calpain inhibitor, calpastatin, during apoptosis.
1998,
Pubmed
Saitoh,
Pro-apoptotic activity and mono-/diubiquitylation of Xenopus Bid in egg extracts.
2009,
Pubmed
,
Xenbase
Schroeter,
c-Jun N-terminal kinase (JNK)-mediated modulation of brain mitochondria function: new target proteins for JNK signalling in mitochondrion-dependent apoptosis.
2003,
Pubmed
Slee,
Cleavage of BID during cytotoxic drug and UV radiation-induced apoptosis occurs downstream of the point of Bcl-2 action and is catalysed by caspase-3: a potential feedback loop for amplification of apoptosis-associated mitochondrial cytochrome c release.
2000,
Pubmed
Song,
Cascleave: towards more accurate prediction of caspase substrate cleavage sites.
2010,
Pubmed
Srivastava,
Deletion of the loop region of Bcl-2 completely blocks paclitaxel-induced apoptosis.
1999,
Pubmed
Tait,
Apoptosis induction by Bid requires unconventional ubiquitination and degradation of its N-terminal fragment.
2007,
Pubmed
Tan,
A novel BH3-like domain in BID is required for intramolecular interaction and autoinhibition of pro-apoptotic activity.
1999,
Pubmed
Thomas,
Direct block of hERG potassium channels by the protein kinase C inhibitor bisindolylmaleimide I (GF109203X).
2004,
Pubmed
,
Xenbase
Thomas,
High-affinity blockade of human ether-a-go-go-related gene human cardiac potassium channels by the novel antiarrhythmic drug BRL-32872.
2001,
Pubmed
,
Xenbase
Tsuruta,
JNK promotes Bax translocation to mitochondria through phosphorylation of 14-3-3 proteins.
2004,
Pubmed
Valentijn,
Translocation of full-length Bid to mitochondria during anoikis.
2004,
Pubmed
Ventura,
Chemical genetic analysis of the time course of signal transduction by JNK.
2006,
Pubmed
Wang,
BID: a novel BH3 domain-only death agonist.
1996,
Pubmed
Wang,
Caspase-mediated fragmentation of calpain inhibitor protein calpastatin during apoptosis.
1998,
Pubmed
Weston,
The JNK signal transduction pathway.
2007,
Pubmed
Yamamoto,
BCL-2 is phosphorylated and inactivated by an ASK1/Jun N-terminal protein kinase pathway normally activated at G(2)/M.
1999,
Pubmed
Yamanaka,
JNK functions in the non-canonical Wnt pathway to regulate convergent extension movements in vertebrates.
2002,
Pubmed
,
Xenbase
