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XB-ART-51486
Nat Commun July 8, 2014; 5 5240.
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FAK transduces extracellular forces that orient the mitotic spindle and control tissue morphogenesis.

Petridou NI , Skourides PA .


Abstract
Spindle orientation is critical for proper morphogenesis of organs and tissues as well as for the maintenance of tissue morphology. Although significant progress has been made in understanding the mechanisms linking the cell cortex to the spindle and the well-documented role that extracellular forces play in spindle orientation, how such forces are transduced to the cortex remains poorly understood. Here we report that focal adhesion kinase (FAK) is necessary for correct spindle orientation and as a result, indispensable for proper epithelial morphogenesis in the vertebrate embryo. We show that FAK''s role in spindle orientation is dependent on its ability to localize at focal adhesions and its interaction with paxillin, but is kinase activity independent. Finally, we present evidence that FAK is required for external force-induced spindle reorientation, suggesting that FAK''s involvement in this process stems from a role in the transduction of external forces to the cell cortex.

PubMed ID: 25341507
Article link: Nat Commun


Species referenced: Xenopus
Genes referenced: ctnnb1 gnl3 gpsm2 nr3c1 numa1 pard6b ptk2 pxn tbxt tjp1
Antibodies: H3f3a Ab9 HA Ab6 Prkcz Ab1 Pxn Ab3 Tjp1 Ab1 Tubb Ab1
Morpholinos: ptk2 MO1 pxn MO1


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