XB-ART-51667
J Membr Biol
2016 Jun 01;2493:239-49. doi: 10.1007/s00232-015-9863-0.
Show Gene links
Show Anatomy links
Caveolin-1 Sensitivity of Excitatory Amino Acid Transporters EAAT1, EAAT2, EAAT3, and EAAT4.
Abousaab A
,
Warsi J
,
Elvira B
,
Lang F
.
???displayArticle.abstract???
Excitatory amino acid transporters EAAT1 (SLC1A3), EAAT2 (SLC1A2), EAAT3 (SLC1A1), and EAAT4 (SLC1A6) serve to clear L-glutamate from the synaptic cleft and are thus important for the limitation of neuronal excitation. EAAT3 has previously been shown to form complexes with caveolin-1, a major component of caveolae, which participate in the regulation of transport proteins. The present study explored the impact of caveolin-1 on electrogenic transport by excitatory amino acid transporter isoforms EAAT1-4. To this end cRNA encoding EAAT1, EAAT2, EAAT3, or EAAT4 was injected into Xenopus oocytes without or with additional injection of cRNA encoding caveolin-1. The L-glutamate (2 mM)-induced inward current (I Glu) was taken as a measure of glutamate transport. As a result, I Glu was observed in EAAT1-, EAAT2-, EAAT3-, or EAAT4-expressing oocytes but not in water-injected oocytes, and was significantly decreased by coexpression of caveolin-1. Caveolin-1 decreased significantly the maximal transport rate. Treatment of EAATs-expressing oocytes with brefeldin A (5 µM) was followed by a decrease in conductance, which was similar in oocytes expressing EAAT together with caveolin-1 as in oocytes expressing EAAT1-4 alone. Thus, caveolin-1 apparently does not accelerate transporter protein retrieval from the cell membrane. In conclusion, caveolin-1 is a powerful negative regulator of the excitatory glutamate transporters EAAT1, EAAT2, EAAT3, and EAAT4.
???displayArticle.pubmedLink??? 26690923
???displayArticle.link??? J Membr Biol
Species referenced: Xenopus laevis
Genes referenced: cav1 slc1a1 slc1a2 slc1a3 slc1a6
References [+] :
Abousaab,
Down-Regulation of Excitatory Amino Acid Transporters EAAT1 and EAAT2 by the Kinases SPAK and OSR1.
2015, Pubmed,
Xenbase
Abousaab, Down-Regulation of Excitatory Amino Acid Transporters EAAT1 and EAAT2 by the Kinases SPAK and OSR1. 2015, Pubmed , Xenbase
Ackerman, Defining a new paradigm for human arrhythmia syndromes: phenotypic manifestations of gene mutations in ion channel- and transporter-associated proteins. 2010, Pubmed
Ahmed, SPAK Sensitive Regulation of the Epithelial Na Channel ENaC. 2015, Pubmed , Xenbase
Ahmed, Down-regulation of inwardly rectifying Kir2.1 K+ channels by human parvovirus B19 capsid protein VP1. 2015, Pubmed , Xenbase
Alesutan, AMP-activated protein kinase α1 regulates cardiac gap junction protein connexin 43 and electrical remodeling following pressure overload. 2015, Pubmed , Xenbase
Almilaji, Klotho sensitivity of the neuronal excitatory amino acid transporters EAAT3 and EAAT4. 2013, Pubmed , Xenbase
Almilaji, Regulation of the voltage gated K channel Kv1.3 by recombinant human klotho protein. 2014, Pubmed , Xenbase
Almilaji, Upregulation of the creatine transporter Slc6A8 by Klotho. 2014, Pubmed , Xenbase
Amara, Excitatory amino acid transporters: keeping up with glutamate. 2002, Pubmed
Bailey, Loss-of-function mutations in the glutamate transporter SLC1A1 cause human dicarboxylic aminoaciduria. 2011, Pubmed , Xenbase
Barnett, Antisense knockdown of GLAST, a glial glutamate transporter, compromises retinal function. 2000, Pubmed
Beart, Transporters for L-glutamate: an update on their molecular pharmacology and pathological involvement. 2007, Pubmed
Berger, Comparative analysis of glutamate transporter expression in rat brain using differential double in situ hybridization. 1998, Pubmed
Boycott, Hypoxia suppresses astrocyte glutamate transport independently of amyloid formation. 2007, Pubmed
Brown, Sensing, signaling and sorting events in kidney epithelial cell physiology. 2009, Pubmed
Chidlow, Caveolae, caveolins, and cavins: complex control of cellular signalling and inflammation. 2010, Pubmed
Cholet, Similar perisynaptic glial localization for the Na+,K+-ATPase alpha 2 subunit and the glutamate transporters GLAST and GLT-1 in the rat somatosensory cortex. 2002, Pubmed
Clements, The time course of glutamate in the synaptic cleft. 1992, Pubmed
Deng, Association study of polymorphisms in the glutamate transporter genes SLC1A1, SLC1A3, and SLC1A6 with schizophrenia. 2007, Pubmed
Derouiche, Coincidence of L-glutamate/L-aspartate transporter (GLAST) and glutamine synthetase (GS) immunoreactions in retinal glia: evidence for coupling of GLAST and GS in transmitter clearance. 1995, Pubmed
Desilva, Glutamate transporter EAAT2 expression is up-regulated in reactive astrocytes in human periventricular leukomalacia. 2008, Pubmed
Domercq, Expression of glutamate transporters in rat optic nerve oligodendrocytes. 1999, Pubmed
Domercq, Expression of glutamate transporters in the adult bovine corpus callosum. 1999, Pubmed
Dowd, Comparison of Na+-dependent glutamate transport activity in synaptosomes, C6 glioma, and Xenopus oocytes expressing excitatory amino acid carrier 1 (EAAC1). 1996, Pubmed , Xenbase
Dzubay, The concentration of synaptically released glutamate outside of the climbing fiber-Purkinje cell synaptic cleft. 1999, Pubmed
Elvira, SPAK and OSR1 Sensitive Cell Membrane Protein Abundance and Activity of KCNQ1/E1 K+ Channels. 2015, Pubmed , Xenbase
Elvira, SPAK and OSR1 dependent down-regulation of murine renal outer medullary K channel ROMK1. 2014, Pubmed , Xenbase
Elvira, Up-regulation of Na(+)-coupled glucose transporter SGLT1 by caveolin-1. 2013, Pubmed , Xenbase
Fang, The chemokine, macrophage inflammatory protein-2γ, reduces the expression of glutamate transporter-1 on astrocytes and increases neuronal sensitivity to glutamate excitotoxicity. 2012, Pubmed
Fezai, Negative regulation of the creatine transporter SLC6A8 by SPAK and OSR1. 2014, Pubmed , Xenbase
Fezai, Up-Regulation of Intestinal Phosphate Transporter NaPi-IIb (SLC34A2) by the Kinases SPAK and OSR1. 2015, Pubmed , Xenbase
Foran, Glutamate transporters and the excitotoxic path to motor neuron degeneration in amyotrophic lateral sclerosis. 2009, Pubmed
Furness, Immunocytochemical localization of a high-affinity glutamate-aspartate transporter, GLAST, in the rat and guinea-pig cochlea. 1997, Pubmed
Furuta, Glutamate transporter protein subtypes are expressed differentially during rat CNS development. 1997, Pubmed
Gaillet, Transient increase in the high affinity [3H]-L-glutamate uptake activity during in vitro development of hippocampal neurons in culture. 2001, Pubmed
Gibb, A caspase-3-cleaved fragment of the glial glutamate transporter EAAT2 is sumoylated and targeted to promyelocytic leukemia nuclear bodies in mutant SOD1-linked amyotrophic lateral sclerosis. 2007, Pubmed
González, Caveolin-1 regulates the delivery and endocytosis of the glutamate transporter, excitatory amino acid carrier 1. 2007, Pubmed
Gray, Glutamate does not play a major role in controlling bone growth. 2001, Pubmed
Hosseinzadeh, Down-regulation of the epithelial Na⁺ channel ENaC by Janus kinase 2. 2014, Pubmed , Xenbase
Hosseinzadeh, Up-regulation of Kv1.3 channels by janus kinase 2. 2015, Pubmed , Xenbase
Hosseinzadeh, The Role of Janus Kinase 3 in the Regulation of Na⁺/K⁺ ATPase under Energy Depletion. 2015, Pubmed , Xenbase
Huang, Climbing fiber activation of EAAT4 transporters and kainate receptors in cerebellar Purkinje cells. 2004, Pubmed
Hurtado, Delayed post-ischemic administration of CDP-choline increases EAAT2 association to lipid rafts and affords neuroprotection in experimental stroke. 2008, Pubmed
Karlsson, Loss of glial glutamate and aspartate transporter (excitatory amino acid transporter 1) causes locomotor hyperactivity and exaggerated responses to psychotomimetics: rescue by haloperidol and metabotropic glutamate 2/3 agonist. 2008, Pubmed
Karlsson, Assessment of glutamate transporter GLAST (EAAT1)-deficient mice for phenotypes relevant to the negative and executive/cognitive symptoms of schizophrenia. 2009, Pubmed
Lang, Molecular mechanisms of schizophrenia. 2007, Pubmed
Lawton, Localization of the glutamate-aspartate transporter, GLAST, in rat taste buds. 2000, Pubmed
Lee, Localization, transport, and uptake of D-aspartate in the rat adrenal and pituitary glands. 2001, Pubmed
Li, Identification of a glutamate/aspartate transporter in the rat cochlea. 1994, Pubmed
Li, Glutamate transporter alterations in Alzheimer disease are possibly associated with abnormal APP expression. 1997, Pubmed
Maragakis, Glutamate transporter expression and function in human glial progenitors. 2004, Pubmed
Mason, Mechanically regulated expression of a neural glutamate transporter in bone: a role for excitatory amino acids as osteotropic agents? 1997, Pubmed
Matsumoto, The -181 A/C polymorphism in the excitatory amino acid transporter-2 gene promoter affects the personality trait of reward dependence in healthy subjects. 2007, Pubmed
Milton, Expression of the glial glutamate transporter EAAT2 in the human CNS: an immunohistochemical study. 1997, Pubmed
Miralles, Na+ dependent glutamate transporters (EAAT1, EAAT2, and EAAT3) in primary astrocyte cultures: effect of oxidative stress. 2001, Pubmed
Munoz, Up-regulation of Kir2.1 (KCNJ2) by the serum & glucocorticoid inducible SGK3. 2014, Pubmed , Xenbase
Münch, Chemical hypoxia facilitates alternative splicing of EAAT2 in presymptomatic APP23 transgenic mice. 2008, Pubmed
O'Kane, Na(+)-dependent glutamate transporters (EAAT1, EAAT2, and EAAT3) of the blood-brain barrier. A mechanism for glutamate removal. 1999, Pubmed
Ong, The dynamic complexity of the TRPC1 channelosome. 2011, Pubmed
Pampliega, Association of an EAAT2 polymorphism with higher glutamate concentration in relapsing multiple sclerosis. 2008, Pubmed
Pavlides, Atherosclerosis, caveolae and caveolin-1. 2012, Pubmed
Peghini, Glutamate transporter EAAC-1-deficient mice develop dicarboxylic aminoaciduria and behavioral abnormalities but no neurodegeneration. 1997, Pubmed
Pilch, Fat caves: caveolae, lipid trafficking and lipid metabolism in adipocytes. 2011, Pubmed
Rakhade, Activity-dependent gene expression correlates with interictal spiking in human neocortical epilepsy. 2007, Pubmed
Rakhade, Focal reduction of neuronal glutamate transporters in human neocortical epilepsy. 2008, Pubmed
Redecker, Immunohistochemical study of the glutamate transporter proteins GLT-1 and GLAST in rat and gerbil pineal gland. 2000, Pubmed
Rothstein, Decreased glutamate transport by the brain and spinal cord in amyotrophic lateral sclerosis. 1992, Pubmed
Rothstein, Localization of neuronal and glial glutamate transporters. 1994, Pubmed
Rumbaugh, Ceftriaxone protects against the neurotoxicity of human immunodeficiency virus proteins. 2007, Pubmed
Schniepp, Retinal colocalization and in vitro interaction of the glutamate transporter EAAT3 and the serum- and glucocorticoid-inducible kinase SGK1 [correction]. 2004, Pubmed , Xenbase
Sheldon, The role of glutamate transporters in neurodegenerative diseases and potential opportunities for intervention. 2007, Pubmed
Sowa, Caveolae, caveolins, cavins, and endothelial cell function: new insights. 2012, Pubmed
Stern, Caveolin regulation of neuronal intracellular signaling. 2010, Pubmed
Takumi, Discrete cellular and subcellular localization of glutamine synthetase and the glutamate transporter GLAST in the rat vestibular end organ. 1997, Pubmed
Tian, Translational control of glial glutamate transporter EAAT2 expression. 2007, Pubmed
Ullensvang, Differential developmental expression of the two rat brain glutamate transporter proteins GLAST and GLT. 1997, Pubmed
Utsumi, Differential expression patterns of three glutamate transporters (GLAST, GLT1 and EAAC1) in the rat main olfactory bulb. 2001, Pubmed
Vercellino, Altered glutamate reuptake in relapsing-remitting and secondary progressive multiple sclerosis cortex: correlation with microglia infiltration, demyelination, and neuronal and synaptic damage. 2007, Pubmed
Warsi, Regulation of Large Conductance Voltage-and Ca2+-Activated K+ Channels by the Janus Kinase JAK3. 2015, Pubmed , Xenbase
Warsi, Regulation of Voltage-Gated K+ Channel Kv1.5 by the Janus Kinase JAK3. 2015, Pubmed , Xenbase
Warsi, Downregulation of peptide transporters PEPT1 and PEPT2 by oxidative stress responsive kinase OSR1. 2014, Pubmed , Xenbase
Warsi, SPAK dependent regulation of peptide transporters PEPT1 and PEPT2. 2014, Pubmed , Xenbase
Warsi, Regulation of ClC-2 activity by SPAK and OSR1. 2014, Pubmed , Xenbase
Zschocke, Caveolin and GLT-1 gene expression is reciprocally regulated in primary astrocytes: association of GLT-1 with non-caveolar lipid rafts. 2005, Pubmed
van Landeghem, Expression of PACAP and glutamate transporter proteins in satellite oligodendrocytes of the human CNS. 2007, Pubmed
van Landeghem, Decreased expression of glutamate transporters in astrocytes after human traumatic brain injury. 2006, Pubmed