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Development March 1, 2016; 143 (5): 864-71.

A gradient of maternal Bicaudal-C controls vertebrate embryogenesis via translational repression of mRNAs encoding cell fate regulators.

Park S , Blaser S , Marchal MA , Houston DW , Sheets MD .

Vertebrate Bicaudal-C (Bicc1) has important biological roles in the formation and homeostasis of multiple organs, but direct experiments to address the role of maternal Bicc1 in early vertebrate embryogenesis have not been reported. Here, we use antisense phosphorothioate-modified oligonucleotides and the host-transfer technique to eliminate specifically maternal stores of both bicc1 mRNA and Bicc1 protein from Xenopus laevis eggs. Fertilization of these Bicc1-depleted eggs produced embryos with an excess of dorsal-anterior structures and overexpressed organizer-specific genes, indicating that maternal Bicc1 is crucial for normal embryonic patterning of the vertebrate embryo. Bicc1 is an RNA-binding protein with robust translational repression function. Here, we show that the maternal mRNA encoding the cell-fate regulatory protein Wnt11b is a direct target of Bicc1-mediated repression. It is well established that the Wnt signaling pathway is crucial to vertebrate embryogenesis. Thus, the work presented here links the molecular function of Bicc1 in mRNA target-specific translation repression to its biological role in the maternally controlled stages of vertebrate embryogenesis.

PubMed ID: 26811381
PMC ID: PMC4813341
Article link: Development
Grant support: [+]

Species referenced: Xenopus laevis
Genes referenced: bicc1 ccnb1 dand5 ddx5 gsc nodal3.1 nog odc1 sia1 tdgf1.3 ventx1.2 wnt11b wnt8a
Antibodies: Bicc1 Ab1

Article Images: [+] show captions
References [+] :
Bates, Coco regulates dorsoventral specification of germ layers via inhibition of TGFβ signalling. 2013, Pubmed, Xenbase