Malunga LN et al. (2016), Inhibition of Intestinal α-Glucosidase and Gluc...

XB-ART-52016

J Nutr Metab 2016 Jan 01;2016:1932532. doi: 10.1155/2016/1932532.

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Inhibition of Intestinal α-Glucosidase and Glucose Absorption by Feruloylated Arabinoxylan Mono- and Oligosaccharides from Corn Bran and Wheat Aleurone.

Malunga LN , Eck P , Beta T .

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The effect of feruloylated arabinoxylan mono- and oligosaccharides (FAXmo) on mammalian α-glucosidase and glucose transporters was investigated using human Caco-2 cells, rat intestinal acetone powder, and Xenopus laevis oocytes. The isolated FAXmo from wheat aleurone and corn bran were identified to have degree of polymerization (DP) of 4 and 1, respectively, by HPLC-MS. Both FAXmo extracts were effective inhibitors of sucrase and maltase functions of the α-glucosidase. The IC50 for FAXmo extracts on Caco-2 cells and rat intestinal α-glucosidase was 1.03-1.65 mg/mL and 2.6-6.5 mg/mL, respectively. Similarly, glucose uptake in Caco-2 cells was inhibited up to 40%. The inhibitory effect of FAXmo was dependent on their ferulic acid (FA) content (R = 0.95). Sodium independent glucose transporter 2 (GLUT2) activity was completely inhibited by FAXmo in oocytes injected to express GLUT2. Our results suggest that ferulic acid and feruloylated arabinoxylan mono-/oligosaccharides have potential for use in diabetes management.

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Species referenced: Xenopus laevis
Genes referenced: slc2a2

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References [+] :

Allerdings, Isolation and structural identification of complex feruloylated heteroxylan side-chains from maize bran. 2006, Pubmed

	Figure 1. HPLC chromatograms of feruloyl mono- and oligosaccharide arabinoxylans from corn bran (a) and wheat aleurone (b).
	Figure 2. Negative ion mass spectra of feruloyl mono/oligosaccharide arabinoxylans [M-H]− from corn bran (a) and wheat aleurone (b and c). Compounds eluting at 26.12 and 27.57 minutes in corn bran UV spectra had the same molecular weight [M-H]− of 325 (a). Also compounds eluting at 13.28 minutes in wheat aleurone UV spectra had m/z = 721 (b) whereas those at 14.57, 15.78, 16.92, and 17.63 minutes had the same m/z = 853 (c).
	Figure 3. MSMS spectra of feruloyl mono- and oligosaccharide arabinoxylans [M-H]− from corn bran and wheat aleurone: (a) m/z = 325; (b) m/z = 853; and (c) m/z = 721.
	Figure 4. Effect of ferulic acid and feruloylated arabinoxylan mono- and oligosaccharides extracts on glucose uptake in Caco-2 cells in sodium plus medium. Values are presented as mean ± standard deviation (n = 6). Data with the same superscript are not significantly different at p ≤ 0.05. FAXmo means feruloylated arabinoxylan mono- and oligosaccharide extract.
	Figure 5. Effect of ferulic acid and feruloylated arabinoxylan mono- and oligosaccharides extracts on glucose uptake in Caco-2 cells in sodium-free medium. Values are presented as mean ± standard deviation (n = 6). Data with the same superscript are not significantly different at p ≤ 0.05. FAXmo means feruloylated arabinoxylan mono- and oligosaccharide extract.
	Figure 6. Glucose uptake inhibition (%) by ferulic acid in Caco-2 cells. A plot to demonstrate that ferulic acid is the active site for inhibiting glucose absorption in Caco-2 cells. Inhibition percent was calculated from means of glucose uptake in the presence of ferulic acid or ferulic acid sugar esters.