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Sulfate transporters involved in sulfate secretion in the kidney are localized in the renal proximal tubule II of the elephant fish (Callorhinchus milii).
Hasegawa K
,
Kato A
,
Watanabe T
,
Takagi W
,
Romero MF
,
Bell JD
,
Toop T
,
Donald JA
,
Hyodo S
.
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Most vertebrates, including cartilaginous fishes, maintain their plasma SO4 (2-) concentration ([SO4 (2-)]) within a narrow range of 0.2-1 mM. As seawater has a [SO4 (2-)] about 40 times higher than that of the plasma, SO4 (2-) excretion is the major role of kidneys in marine teleost fishes. It has been suggested that cartilaginous fishes also excrete excess SO4 (2-) via the kidney. However, little is known about the underlying mechanisms for SO4 (2-) transport in cartilaginous fish, largely due to the extraordinarily elaborate four-loop configuration of the nephron, which consists of at least 10 morphologically distinguishable segments. In the present study, we determined cDNA sequences from the kidney of holocephalan elephant fish (Callorhinchus milii) that encoded solute carrier family 26 member 1 (Slc26a1) and member 6 (Slc26a6), which are SO4 (2-) transporters that are expressed in mammalian and teleost kidneys. Elephant fish Slc26a1 (cmSlc26a1) and cmSlc26a6 mRNAs were coexpressed in the proximal II (PII) segment of the nephron, which comprises the second loop in the sinus zone. Functional analyses using Xenopus oocytes and the results of immunohistochemistry revealed that cmSlc26a1 is a basolaterally located electroneutral SO4 (2-) transporter, while cmSlc26a6 is an apically located, electrogenic Cl(-)/SO4 (2-) exchanger. In addition, we found that both cmSlc26a1 and cmSlc26a6 were abundantly expressed in the kidney of embryos; SO4 (2-) was concentrated in a bladder-like structure of elephant fish embryos. Our results demonstrated that the PII segment of the nephron contributes to the secretion of excess SO4 (2-) by the kidney of elephant fish. Possible mechanisms for SO4 (2-) secretion in the PII segment are discussed.
Alper,
The SLC26 gene family of anion transporters and channels.
2013, Pubmed
Alper,
The SLC26 gene family of anion transporters and channels.
2013,
Pubmed
Beyenbach,
Renal proximal tubule of flounder. I. Physiological properties.
1986,
Pubmed
Borghese,
Studies on the nephron of an elasmobranch fish Scyliorhinus stellaris (L.).
1966,
Pubmed
Cassola,
The intracellular chloride activity of rat kidney proximal tubular cells.
1983,
Pubmed
Choe,
The putative mechanism of Na(+) absorption in euryhaline elasmobranchs exists in the gills of a stenohaline marine elasmobranch, Squalus acanthias.
2007,
Pubmed
Choe,
NHE3 in an ancestral vertebrate: primary sequence, distribution, localization, and function in gills.
2005,
Pubmed
Didier,
Embryonic staging and external features of development of the Chimaeroid fish, Callorhinchus milii (Holocephali, Callorhinchidae).
1998,
Pubmed
Dixon,
A novel cDNA restores reduced folate carrier activity and methotrexate sensitivity to transport deficient cells.
1994,
Pubmed
Fong,
CFTR-SLC26 transporter interactions in epithelia.
2012,
Pubmed
Friedman,
Diluting segment in kidney of dogfish shark. I. Localization and characterization of chloride absorption.
1990,
Pubmed
Gamba,
Molecular cloning, primary structure, and characterization of two members of the mammalian electroneutral sodium-(potassium)-chloride cotransporter family expressed in kidney.
1994,
Pubmed
,
Xenbase
Gorbunov,
Molecular architecture and the structural basis for anion interaction in prestin and SLC26 transporters.
2014,
Pubmed
Hebert,
Molecular physiology of cation-coupled Cl- cotransport: the SLC12 family.
2004,
Pubmed
Hyodo,
Osmoregulation in elephant fish Callorhinchus milii (Holocephali), with special reference to the rectal gland.
2007,
Pubmed
Hyodo,
A facilitative urea transporter is localized in the renal collecting tubule of the dogfish Triakis scyllia.
2004,
Pubmed
Hyodo,
Morphological and functional characteristics of the kidney of cartilaginous fishes: with special reference to urea reabsorption.
2014,
Pubmed
Ishibashi,
Intracellular chloride activity of rabbit proximal straight tubule perfused in vitro.
1988,
Pubmed
Islam,
Identification and lateral membrane localization of cyclin M3, likely to be involved in renal Mg2+ handling in seawater fish.
2014,
Pubmed
,
Xenbase
Jones,
InterProScan 5: genome-scale protein function classification.
2014,
Pubmed
Kakumura,
Morphological and molecular investigations of the holocephalan elephant fish nephron: the existence of a countercurrent-like configuration and two separate diluting segments in the distal tubule.
2015,
Pubmed
Karniski,
Immunolocalization of sat-1 sulfate/oxalate/bicarbonate anion exchanger in the rat kidney.
1998,
Pubmed
Kato,
Identification of renal transporters involved in sulfate excretion in marine teleost fish.
2009,
Pubmed
,
Xenbase
Katoh,
Cloning of rainbow trout SLC26A1: involvement in renal sulfate secretion.
2006,
Pubmed
Knauf,
Identification of a chloride-formate exchanger expressed on the brush border membrane of renal proximal tubule cells.
2001,
Pubmed
,
Xenbase
Krapf,
Estimation of intracellular chloride activity in isolated perfused rabbit proximal convoluted tubules using a fluorescent indicator.
1988,
Pubmed
Krick,
Ability of sat-1 to transport sulfate, bicarbonate, or oxalate under physiological conditions.
2009,
Pubmed
,
Xenbase
Kuo,
Oxalate transport via the sulfate/HCO3 exchanger in rabbit renal basolateral membrane vesicles.
1988,
Pubmed
Kurita,
Identification of intestinal bicarbonate transporters involved in formation of carbonate precipitates to stimulate water absorption in marine teleost fish.
2008,
Pubmed
,
Xenbase
Lacy,
The elasmobranch kidney. II. Sequence and structure of the nephrons.
1985,
Pubmed
Lee,
The mouse sulfate anion transporter gene Sat1 (Slc26a1): cloning, tissue distribution, gene structure, functional characterization, and transcriptional regulation thyroid hormone.
2003,
Pubmed
,
Xenbase
Lohi,
Mapping of five new putative anion transporter genes in human and characterization of SLC26A6, a candidate gene for pancreatic anion exchanger.
2000,
Pubmed
Markovich,
Physiological roles and regulation of mammalian sulfate transporters.
2001,
Pubmed
Markovich,
Specificity and regulation of renal sulfate transporters.
2007,
Pubmed
Markovich,
Slc13a1 and Slc26a1 KO models reveal physiological roles of anion transporters.
2012,
Pubmed
McCurley,
Characterization of housekeeping genes in zebrafish: male-female differences and effects of tissue type, developmental stage and chemical treatment.
2008,
Pubmed
Nakada,
Roles of Slc13a1 and Slc26a1 sulfate transporters of eel kidney in sulfate homeostasis and osmoregulation in freshwater.
2005,
Pubmed
,
Xenbase
Ohana,
Determinants of coupled transport and uncoupled current by the electrogenic SLC26 transporters.
2011,
Pubmed
Pelis,
Role of tubular secretion and carbonic anhydrase in vertebrate renal sulfate excretion.
2004,
Pubmed
Petrovic,
Identification of an apical Cl-/HCO-3 exchanger in rat kidney proximal tubule.
2003,
Pubmed
,
Xenbase
Piermarini,
Pendrin immunoreactivity in the gill epithelium of a euryhaline elasmobranch.
2002,
Pubmed
Pritchard,
Renal sulfate transport at the basolateral membrane is mediated by anion exchange.
1983,
Pubmed
Regeer,
Characterization of the human sulfate anion transporter (hsat-1) protein and gene (SAT1; SLC26A1).
2003,
Pubmed
,
Xenbase
Renfro,
Renal sulfate secretion is carbonic anhydrase dependent in a marine teleost, Pleuronectes americanus.
1999,
Pubmed
Romero,
Cloning and characterization of a Na+-driven anion exchanger (NDAE1). A new bicarbonate transporter.
2000,
Pubmed
,
Xenbase
Romero,
Cloning and functional expression of rNBC, an electrogenic Na(+)-HCO3- cotransporter from rat kidney.
1998,
Pubmed
,
Xenbase
Satoh,
Functional analysis of diastrophic dysplasia sulfate transporter. Its involvement in growth regulation of chondrocytes mediated by sulfated proteoglycans.
1998,
Pubmed
,
Xenbase
Stolte,
Renal tubule ion transport and collecting duct function in the elasmobranch little skate, Raja erinacea.
1977,
Pubmed
Takabe,
Morphological and functional characterization of a novel Na+/K+-ATPase-immunoreactive, follicle-like structure on the gill septum of Japanese banded houndshark, Triakis scyllium.
2012,
Pubmed
Takagi,
Hepatic and extrahepatic distribution of ornithine urea cycle enzymes in holocephalan elephant fish (Callorhinchus milii).
2012,
Pubmed
Takagi,
Urea-based osmoregulation in the developing embryo of oviparous cartilaginous fish (Callorhinchus milii): contribution of the extraembryonic yolk sac during the early developmental period.
2014,
Pubmed
Thompson,
CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice.
1994,
Pubmed
Venkatesh,
Survey sequencing and comparative analysis of the elephant shark (Callorhinchus milii) genome.
2007,
Pubmed
Venkatesh,
Elephant shark genome provides unique insights into gnathostome evolution.
2014,
Pubmed
Wang,
Renal and intestinal transport defects in Slc26a6-null mice.
2005,
Pubmed
Watanabe,
Molecular physiology and functional morphology of SO₄²⁻ excretion by the kidney of seawater-adapted eels.
2011,
Pubmed
Xie,
Molecular characterization of the murine Slc26a6 anion exchanger: functional comparison with Slc26a1.
2002,
Pubmed
,
Xenbase
Yamaguchi,
Subcellular distribution of urea transporter in the collecting tubule of shark kidney is dependent on environmental salinity.
2009,
Pubmed
