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J Vis Exp April 12, 2016; (110): e53949.

Assessing Primary Neurogenesis in Xenopus Embryos Using Immunostaining.

Zhang S , Li J , Lea R , Amaya E .

Primary neurogenesis is a dynamic and complex process during embryonic development that sets up the initial layout of the central nervous system. During this process, a portion of neural stem cells undergo differentiation and give rise to the first populations of differentiated primary neurons within the nascent central nervous system. Several vertebrate model organisms have been used to explore the mechanisms of neural cell fate specification, patterning, and differentiation. Among these is the African clawed frog, Xenopus, which provides a powerful system for investigating the molecular and cellular mechanisms responsible for primary neurogenesis due to its rapid and accessible development and ease of embryological and molecular manipulations. Here, we present a convenient and rapid method to observe the different populations of neuronal cells within Xenopus central nervous system. Using antibody staining and immunofluorescence on sections of Xenopus embryos, we are able to observe the locations of neural stem cells and differentiated primary neurons during primary neurogenesis.

PubMed ID: 27166855
PMC ID: PMC4941913
Article link: J Vis Exp
Grant support: [+]

Species referenced: Xenopus laevis
Genes referenced: myt1 sox3 tubb2b

Article Images: [+] show captions
References [+] :
Bellefroid, X-MyT1, a Xenopus C2HC-type zinc finger protein with a regulatory function in neuronal differentiation. 1997, Pubmed, Xenbase