XB-ART-52203
Nat Commun
2015 Dec 17;6:10173. doi: 10.1038/ncomms10173.
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Kv3.1 uses a timely resurgent K(+) current to secure action potential repolarization.
Labro AJ
,
Priest MF
,
Lacroix JJ
,
Snyders DJ
,
Bezanilla F
.
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High-frequency action potential (AP) transmission is essential for rapid information processing in the central nervous system. Voltage-dependent Kv3 channels play an important role in this process thanks to their high activation threshold and fast closure kinetics, which reduce the neuron's refractory period. However, premature Kv3 channel closure leads to incomplete membrane repolarization, preventing sustainable AP propagation. Here, we demonstrate that Kv3.1b channels solve this problem by producing resurgent K(+) currents during repolarization, thus ensuring enough repolarizing power to terminate each AP. Unlike previously described resurgent Na(+) and K(+) currents, Kv3.1b's resurgent current does not originate from recovery of channel block or inactivation but results from a unique combination of steep voltage-dependent gating kinetics and ultra-fast voltage-sensor relaxation. These distinct properties are readily transferrable onto an orthologue Kv channel by transplanting the voltage-sensor's S3-S4 loop, providing molecular insights into the mechanism by which Kv3 channels contribute to high-frequency AP transmission.
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F31 NS081954 NINDS NIH HHS , R37 GM030376 NIGMS NIH HHS , R01 GM030376 NIGMS NIH HHS , NIH-NS081954 NINDS NIH HHS , NIH-GM030376 NIGMS NIH HHS
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