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Biochem Biophys Res Commun October 28, 2016; 479 (4): 759-765.

EphA7 modulates apical constriction of hindbrain neuroepithelium during neurulation in Xenopus.

Wang X , Sun J , Li C , Mao B .

Eph receptor tyrosine kinases (RTKs) and their ephrin ligands play multiple roles in the developing nervous system, including cell segregation, axon guidance and synaptic plasticity. Here we report the expression and function of EphA7 in Xenopus hindbrain development. EphA7 is specifically expressed in the hindbrain throughout neurulation in Xenopus embryos. Knockdown of EphA7 by specific morpholino oligonucleotide (MO) disrupted cranial neural tube closure and disturbed apical constriction of hindbrain neuroepithelial cells, indicating weakened cell surface tension. In neural plate explants, EphA7 knockdown inhibited apical filamentous actin (F-actin) accumulation. We further showed that EphA7 is involved in the phosphorylation and activation of focal adhesion kinase (FAK) in vivo and in vitro, a key regulator of actin assembly. Our findings reveal that EphA7 functions as a critical regulator of apical constriction of hindbrain neuroepithelial cells.

PubMed ID: 27693790
Article link: Biochem Biophys Res Commun

Species referenced: Xenopus laevis
Genes referenced: egr2 en2 epha7 fn1 mlc1 ptk2 sox2
Antibodies: Cdh3 Ab1
Morpholinos: epha7 MO1

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