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Cell
2016 Oct 06;1672:498-511.e14. doi: 10.1016/j.cell.2016.09.008.
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Replication-Dependent Unhooking of DNA Interstrand Cross-Links by the NEIL3 Glycosylase.
Semlow DR
,
Zhang J
,
Budzowska M
,
Drohat AC
,
Walter JC
.
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During eukaryotic DNA interstrand cross-link (ICL) repair, cross-links are resolved ("unhooked") by nucleolytic incisions surrounding the lesion. In vertebrates, ICL repair is triggered when replication forks collide with the lesion, leading to FANCI-FANCD2-dependent unhooking and formation of a double-strand break (DSB) intermediate. Using Xenopus egg extracts, we describe here a replication-coupled ICL repair pathway that does not require incisions or FANCI-FANCD2. Instead, the ICL is unhooked when one of the two N-glycosyl bonds forming the cross-link is cleaved by the DNA glycosylase NEIL3. Cleavage by NEIL3 is the primary unhooking mechanism for psoralen and abasic site ICLs. When N-glycosyl bond cleavage is prevented, unhooking occurs via FANCI-FANCD2-dependent incisions. In summary, we identify an incision-independent unhooking mechanism that avoids DSB formation and represents the preferred pathway of ICL repair in a vertebrate cell-free system.
Alt,
Bypass of DNA lesions generated during anticancer treatment with cisplatin by DNA polymerase eta.
2007, Pubmed
Alt,
Bypass of DNA lesions generated during anticancer treatment with cisplatin by DNA polymerase eta.
2007,
Pubmed
Brooks,
Recent advances in the structural mechanisms of DNA glycosylases.
2013,
Pubmed
Budzowska,
Regulation of the Rev1-pol ζ complex during bypass of a DNA interstrand cross-link.
2015,
Pubmed
,
Xenbase
Cimino,
Wavelength dependence for the photoreversal of a psoralen-DNA cross-link.
1986,
Pubmed
Clauson,
Advances in understanding the complex mechanisms of DNA interstrand cross-link repair.
2013,
Pubmed
Couvé,
The human oxidative DNA glycosylase NEIL1 excises psoralen-induced interstrand DNA cross-links in a three-stranded DNA structure.
2009,
Pubmed
Deans,
FANCM connects the genome instability disorders Bloom's Syndrome and Fanconi Anemia.
2009,
Pubmed
Dewar,
The mechanism of DNA replication termination in vertebrates.
2015,
Pubmed
,
Xenbase
Duxin,
What is the DNA repair defect underlying Fanconi anemia?
2015,
Pubmed
,
Xenbase
Duxin,
Repair of a DNA-protein crosslink by replication-coupled proteolysis.
2014,
Pubmed
,
Xenbase
Enoiu,
Construction of plasmids containing site-specific DNA interstrand cross-links for biochemical and cell biological studies.
2012,
Pubmed
Fu,
Selective bypass of a lagging strand roadblock by the eukaryotic replicative DNA helicase.
2011,
Pubmed
,
Xenbase
Garaycoechea,
Genotoxic consequences of endogenous aldehydes on mouse haematopoietic stem cell function.
2012,
Pubmed
Guainazzi,
Synthesis and molecular modeling of a nitrogen mustard DNA interstrand crosslink.
2010,
Pubmed
Hildrestrand,
Expression patterns of Neil3 during embryonic brain development and neoplasia.
2009,
Pubmed
Houghtaling,
Fancd2 functions in a double strand break repair pathway that is distinct from non-homologous end joining.
2005,
Pubmed
Joenje,
The emerging genetic and molecular basis of Fanconi anaemia.
2001,
Pubmed
Klein Douwel,
XPF-ERCC1 acts in Unhooking DNA interstrand crosslinks in cooperation with FANCD2 and FANCP/SLX4.
2014,
Pubmed
,
Xenbase
Knipscheer,
The Fanconi anemia pathway promotes replication-dependent DNA interstrand cross-link repair.
2009,
Pubmed
,
Xenbase
Kottemann,
Fanconi anaemia and the repair of Watson and Crick DNA crosslinks.
2013,
Pubmed
Krokeide,
Human NEIL3 is mainly a monofunctional DNA glycosylase removing spiroimindiohydantoin and guanidinohydantoin.
2013,
Pubmed
Langevin,
Fancd2 counteracts the toxic effects of naturally produced aldehydes in mice.
2011,
Pubmed
Lebofsky,
DNA replication in nucleus-free Xenopus egg extracts.
2009,
Pubmed
,
Xenbase
Lee,
Developmental stage- and DNA damage-specific functions of C. elegans FANCD2.
2007,
Pubmed
Liao,
Biochemical reconstitution of abasic DNA lesion replication in Xenopus extracts.
2007,
Pubmed
,
Xenbase
Liu,
Expression and purification of active mouse and human NEIL3 proteins.
2012,
Pubmed
Liu,
Neil3, the final frontier for the DNA glycosylases that recognize oxidative damage.
2013,
Pubmed
Liu,
Structural characterization of a mouse ortholog of human NEIL3 with a marked preference for single-stranded DNA.
2013,
Pubmed
Liu,
The mouse ortholog of NEIL3 is a functional DNA glycosylase in vitro and in vivo.
2010,
Pubmed
Long,
Mechanism of RAD51-dependent DNA interstrand cross-link repair.
2011,
Pubmed
,
Xenbase
Long,
BRCA1 promotes unloading of the CMG helicase from a stalled DNA replication fork.
2014,
Pubmed
,
Xenbase
Magnaghi,
Covalent and allosteric inhibitors of the ATPase VCP/p97 induce cancer cell death.
2013,
Pubmed
Maiti,
Role of two strictly conserved residues in nucleotide flipping and N-glycosylic bond cleavage by human thymine DNA glycosylase.
2009,
Pubmed
Maric,
Cdc48 and a ubiquitin ligase drive disassembly of the CMG helicase at the end of DNA replication.
2014,
Pubmed
Moreno,
Polyubiquitylation drives replisome disassembly at the termination of DNA replication.
2014,
Pubmed
,
Xenbase
Mullins,
The DNA glycosylase AlkD uses a non-base-flipping mechanism to excise bulky lesions.
2015,
Pubmed
Nakamura,
Endogenous apurinic/apyrimidinic sites in genomic DNA of mammalian tissues.
1999,
Pubmed
Neurauter,
Release from quiescence stimulates the expression of human NEIL3 under the control of the Ras dependent ERK-MAP kinase pathway.
2012,
Pubmed
Pontel,
Endogenous Formaldehyde Is a Hematopoietic Stem Cell Genotoxin and Metabolic Carcinogen.
2015,
Pubmed
Price,
Interstrand DNA-DNA cross-link formation between adenine residues and abasic sites in duplex DNA.
2014,
Pubmed
Price,
Chemical and structural characterization of interstrand cross-links formed between abasic sites and adenine residues in duplex DNA.
2015,
Pubmed
Rolseth,
Loss of Neil3, the major DNA glycosylase activity for removal of hydantoins in single stranded DNA, reduces cellular proliferation and sensitizes cells to genotoxic stress.
2013,
Pubmed
Rothfuss,
Repair kinetics of genomic interstrand DNA cross-links: evidence for DNA double-strand break-dependent activation of the Fanconi anemia/BRCA pathway.
2004,
Pubmed
Räschle,
Mechanism of replication-coupled DNA interstrand crosslink repair.
2008,
Pubmed
,
Xenbase
Räschle,
DNA repair. Proteomics reveals dynamic assembly of repair complexes during bypass of DNA cross-links.
2015,
Pubmed
,
Xenbase
Sejersted,
Endonuclease VIII-like 3 (Neil3) DNA glycosylase promotes neurogenesis induced by hypoxia-ischemia.
2011,
Pubmed
Taniguchi,
Convergence of the fanconi anemia and ataxia telangiectasia signaling pathways.
2002,
Pubmed
Torisu,
Hematopoietic tissue-specific expression of mouse Neil3 for endonuclease VIII-like protein.
2005,
Pubmed
Walter,
Initiation of eukaryotic DNA replication: origin unwinding and sequential chromatin association of Cdc45, RPA, and DNA polymerase alpha.
2000,
Pubmed
,
Xenbase
Wang,
Characterization of a novel DNA glycosylase from S. sahachiroi involved in the reduction and repair of azinomycin B induced DNA damage.
2016,
Pubmed
Williams,
The differences between ICL repair during and outside of S phase.
2013,
Pubmed
Zhang,
Mechanism and regulation of incisions during DNA interstrand cross-link repair.
2014,
Pubmed
Zhang,
DNA interstrand cross-link repair requires replication-fork convergence.
2015,
Pubmed
,
Xenbase
