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XB-ART-52598
Sci Rep January 1, 2016; 6 35264.

CRISPR/Cas9 mediated knockout of rb1 and rbl1 leads to rapid and penetrant retinoblastoma development in Xenopus tropicalis.

Naert T , Colpaert R , Van Nieuwenhuysen T , Dimitrakopoulou D , Leoen J , Haustraete J , Boel A , Steyaert W , Lepez T , Deforce D , Willaert A , Creytens D , Vleminckx K .


Abstract
Retinoblastoma is a pediatric eye tumor in which bi-allelic inactivation of the Retinoblastoma 1 (RB1) gene is the initiating genetic lesion. Although recently curative rates of retinoblastoma have increased, there are at this time no molecular targeted therapies available. This is, in part, due to the lack of highly penetrant and rapid retinoblastoma animal models that facilitate rapid identification of targets that allow therapeutic intervention. Different mouse models are available, all based on genetic deactivation of both Rb1 and Retinoblastoma-like 1 (Rbl1), and each showing different kinetics of retinoblastoma development. Here, we show by CRISPR/Cas9 techniques that similar to the mouse, neither rb1 nor rbl1 single mosaic mutant Xenopus tropicalis develop tumors, whereas rb1/rbl1 double mosaic mutant tadpoles rapidly develop retinoblastoma. Moreover, occasionally presence of pinealoblastoma (trilateral retinoblastoma) was detected. We thus present the first CRISPR/Cas9 mediated cancer model in Xenopus tropicalis and the first genuine genetic non-mammalian retinoblastoma model. The rapid kinetics of our model paves the way for use as a pre-clinical model. Additionally, this retinoblastoma model provides unique possibilities for fast elucidation of novel drug targets by triple multiplex CRISPR/Cas9 gRNA injections (rb1 + rbl1 + modifier gene) in order to address the clinically unmet need of targeted retinoblastoma therapy.

PubMed ID: 27739525
PMC ID: PMC5064383
Article link: Sci Rep


Species referenced: Xenopus tropicalis
Genes referenced: homer1 pcna rb1 rbl1 syk
Antibodies: Pcna Ab1
gRNAs referenced: rb1 gRNA2 rb1 gRNA3 rbl1 gRNA2 rbl1 gRNA3 syk gRNA1

Disease Ontology terms: retinoblastoma
OMIMs: RETINOBLASTOMA; RB1

Article Images: [+] show captions
References [+] :
Abramson, Treatment of Retinoblastoma in 2015: Agreement and Disagreement. 2015, Pubmed