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Anal Bioanal Chem
2017 Mar 01;4097:1789-1795. doi: 10.1007/s00216-016-0122-8.
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Evaluation of a commercial electro-kinetically pumped sheath-flow nanospray interface coupled to an automated capillary zone electrophoresis system.
Peuchen EH
,
Zhu G
,
Sun L
,
Dovichi NJ
.
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Capillary zone electrophoresis-electrospray ionization-mass spectrometry (CZE-ESI-MS) is attracting renewed attention for proteomic and metabolomic analysis. An important reason for this interest is the maturation and commercialization of interfaces for coupling CZE with ESI-MS. One of these interfaces is an electro-kinetically pumped sheath flow nanospray interface developed by the Dovichi group, in which a very low sheath flow is generated based on electroosmosis within a glass emitter. CMP Scientific has commercialized this interface as the EMASS-II ion source. In this work, we compared the performance of the EMASS-II ion source with our in-house system. The performance of the systems is equivalent. We also coupled the EMASS-II ion source with a PrinCE Next|480 capillary electrophoresis autosampler and an Orbitrap mass spectrometer, and analyzed this system's performance in terms of sensitivity, reproducibility, and separation performance for separation of tryptic digests, intact proteins, and amino acids. The system produced reproducible analysis of BSA digest; the RSDs of peptide intensity and migration time across 24 runs were less than 20 and 6%, respectively. The system produced a linear calibration curve of intensity across a 30-fold range of tryptic digest concentration. The combination of a commercial autosampler and electrospray interface efficiently separated amino acids, peptides, and intact proteins, and only required 5 μL of sample for analysis. Graphical Abstract The commercial and locally constructed versions of the interface provide similar numbers of protein identifications from a Xenopus laevis fertilized egg digest.
Cox,
Accurate proteome-wide label-free quantification by delayed normalization and maximal peptide ratio extraction, termed MaxLFQ.
2014, Pubmed
Cox,
Accurate proteome-wide label-free quantification by delayed normalization and maximal peptide ratio extraction, termed MaxLFQ.
2014,
Pubmed
Cox,
Andromeda: a peptide search engine integrated into the MaxQuant environment.
2011,
Pubmed
Cox,
MaxQuant enables high peptide identification rates, individualized p.p.b.-range mass accuracies and proteome-wide protein quantification.
2008,
Pubmed
Han,
Intact mass analysis of monoclonal antibodies by capillary electrophoresis-Mass spectrometry.
2016,
Pubmed
Maxwell,
Twenty years of interface development for capillary electrophoresis-electrospray ionization-mass spectrometry.
2008,
Pubmed
Peuchen,
Optimization and comparison of bottom-up proteomic sample preparation for early-stage Xenopus laevis embryos.
2016,
Pubmed
,
Xenbase
Ramautar,
CE-MS for metabolomics: developments and applications in the period 2012-2014.
2015,
Pubmed
Schiavone,
High speed capillary zone electrophoresis-mass spectrometry via an electrokinetically pumped sheath flow interface for rapid analysis of amino acids and a protein digest.
2015,
Pubmed
Sun,
Capillary Electrophoresis-Mass Spectrometry for the Analysis of Heparin Oligosaccharides and Low Molecular Weight Heparin.
2016,
Pubmed
Sun,
Over 10,000 peptide identifications from the HeLa proteome by using single-shot capillary zone electrophoresis combined with tandem mass spectrometry.
2014,
Pubmed
Sun,
Capillary zone electrophoresis for bottom-up analysis of complex proteomes.
2016,
Pubmed
Sun,
Third-generation electrokinetically pumped sheath-flow nanospray interface with improved stability and sensitivity for automated capillary zone electrophoresis-mass spectrometry analysis of complex proteome digests.
2015,
Pubmed
,
Xenbase
Sun,
Ultrasensitive and fast bottom-up analysis of femtogram amounts of complex proteome digests.
2013,
Pubmed
Whitt,
Capillary electrophoresis to mass spectrometry interface using a porous junction.
2003,
Pubmed
Wojcik,
Simplified capillary electrophoresis nanospray sheath-flow interface for high efficiency and sensitive peptide analysis.
2010,
Pubmed
Wühr,
Deep proteomics of the Xenopus laevis egg using an mRNA-derived reference database.
2014,
Pubmed
,
Xenbase
Zhao,
Fast separation and analysis of reduced monoclonal antibodies with capillary zone electrophoresis coupled to mass spectrometry.
2016,
Pubmed
Zhao,
Coupling capillary zone electrophoresis with electron transfer dissociation and activated ion electron transfer dissociation for top-down proteomics.
2015,
Pubmed
Zhu,
Thermally-initiated free radical polymerization for reproducible production of stable linear polyacrylamide coated capillaries, and their application to proteomic analysis using capillary zone electrophoresis-mass spectrometry.
2016,
Pubmed
