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Mol Cell Biol
2003 Aug 01;2316:5919-27. doi: 10.1128/MCB.23.16.5919-5927.2003.
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Physical and functional interaction between DNA ligase IIIalpha and poly(ADP-Ribose) polymerase 1 in DNA single-strand break repair.
Leppard JB
,
Dong Z
,
Mackey ZB
,
Tomkinson AE
.
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The repair of DNA single-strand breaks in mammalian cells is mediated by poly(ADP-ribose) polymerase 1 (PARP-1), DNA ligase IIIalpha, and XRCC1. Since these proteins are not found in lower eukaryotes, this DNA repair pathway plays a unique role in maintaining genome stability in more complex organisms. XRCC1 not only forms a stable complex with DNA ligase IIIalpha but also interacts with several other DNA repair factors. Here we have used affinity chromatography to identify proteins that associate with DNA ligase III. PARP-1 binds directly to an N-terminal region of DNA ligase III immediately adjacent to its zinc finger. In further studies, we have shown that DNA ligase III also binds directly to poly(ADP-ribose) and preferentially associates with poly(ADP-ribosyl)ated PARP-1 in vitro and in vivo. Our biochemical studies have revealed that the zinc finger of DNA ligase III increases DNA joining in the presence of either poly(ADP-ribosyl)ated PARP-1 or poly(ADP-ribose). This provides a mechanism for the recruitment of the DNA ligase IIIalpha-XRCC1 complex to in vivo DNA single-strand breaks and suggests that the zinc finger of DNA ligase III enables this complex and associated repair factors to locate the strand break in the presence of the negatively charged poly(ADP-ribose) polymer.
Caldecott,
An interaction between the mammalian DNA repair protein XRCC1 and DNA ligase III.
1994, Pubmed,
Xenbase
Caldecott,
An interaction between the mammalian DNA repair protein XRCC1 and DNA ligase III.
1994,
Pubmed
,
Xenbase
Caldecott,
Characterization of the XRCC1-DNA ligase III complex in vitro and its absence from mutant hamster cells.
1995,
Pubmed
,
Xenbase
Caldecott,
XRCC1 polypeptide interacts with DNA polymerase beta and possibly poly (ADP-ribose) polymerase, and DNA ligase III is a novel molecular 'nick-sensor' in vitro.
1996,
Pubmed
,
Xenbase
Callebaut,
From BRCA1 to RAP1: a widespread BRCT module closely associated with DNA repair.
1997,
Pubmed
Chen,
Mammalian DNA ligase III: molecular cloning, chromosomal localization, and expression in spermatocytes undergoing meiotic recombination.
1995,
Pubmed
,
Xenbase
Frosina,
Two pathways for base excision repair in mammalian cells.
1996,
Pubmed
Giner,
Overproduction and large-scale purification of the human poly(ADP-ribose) polymerase using a baculovirus expression system.
1992,
Pubmed
Husain,
Purification and characterization of DNA ligase III from bovine testes. Homology with DNA ligase II and vaccinia DNA ligase.
1995,
Pubmed
,
Xenbase
Koonin,
BRCA1 protein products ... Functional motifs..
1996,
Pubmed
Kubota,
Reconstitution of DNA base excision-repair with purified human proteins: interaction between DNA polymerase beta and the XRCC1 protein.
1996,
Pubmed
Laemmli,
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
1970,
Pubmed
Lakshmipathy,
The human DNA ligase III gene encodes nuclear and mitochondrial proteins.
1999,
Pubmed
,
Xenbase
Lakshmipathy,
Mitochondrial DNA ligase III function is independent of Xrcc1.
2000,
Pubmed
,
Xenbase
Mackey,
An alternative splicing event which occurs in mouse pachytene spermatocytes generates a form of DNA ligase III with distinct biochemical properties that may function in meiotic recombination.
1997,
Pubmed
,
Xenbase
Mackey,
DNA ligase III is recruited to DNA strand breaks by a zinc finger motif homologous to that of poly(ADP-ribose) polymerase. Identification of two functionally distinct DNA binding regions within DNA ligase III.
1999,
Pubmed
,
Xenbase
Marintchev,
Solution structure of the single-strand break repair protein XRCC1 N-terminal domain.
1999,
Pubmed
Masson,
XRCC1 is specifically associated with poly(ADP-ribose) polymerase and negatively regulates its activity following DNA damage.
1998,
Pubmed
,
Xenbase
Moore,
Mutation of a BRCT domain selectively disrupts DNA single-strand break repair in noncycling Chinese hamster ovary cells.
2000,
Pubmed
Nash,
XRCC1 protein interacts with one of two distinct forms of DNA ligase III.
1997,
Pubmed
Perez-Jannotti,
Two forms of mitochondrial DNA ligase III are produced in Xenopus laevis oocytes.
2001,
Pubmed
,
Xenbase
Pleschke,
Poly(ADP-ribose) binds to specific domains in DNA damage checkpoint proteins.
2000,
Pubmed
Ron,
pGSTag--a versatile bacterial expression plasmid for enzymatic labeling of recombinant proteins.
1992,
Pubmed
Satoh,
Role of poly(ADP-ribose) formation in DNA repair.
1992,
Pubmed
Schreiber,
Poly(ADP-ribose) polymerase-2 (PARP-2) is required for efficient base excision DNA repair in association with PARP-1 and XRCC1.
2002,
Pubmed
,
Xenbase
Taylor,
A cell cycle-specific requirement for the XRCC1 BRCT II domain during mammalian DNA strand break repair.
2000,
Pubmed
,
Xenbase
Thompson,
A CHO-cell strain having hypersensitivity to mutagens, a defect in DNA strand-break repair, and an extraordinary baseline frequency of sister-chromatid exchange.
1982,
Pubmed
Thompson,
Molecular cloning of the human XRCC1 gene, which corrects defective DNA strand break repair and sister chromatid exchange.
1990,
Pubmed
Tomkinson,
Structure and function of mammalian DNA ligases.
1998,
Pubmed
Vidal,
XRCC1 coordinates the initial and late stages of DNA abasic site repair through protein-protein interactions.
2001,
Pubmed
Wei,
Molecular cloning and expression of human cDNAs encoding a novel DNA ligase IV and DNA ligase III, an enzyme active in DNA repair and recombination.
1995,
Pubmed
,
Xenbase
Whitehouse,
XRCC1 stimulates human polynucleotide kinase activity at damaged DNA termini and accelerates DNA single-strand break repair.
2001,
Pubmed
,
Xenbase
Wu,
A 17S multiprotein form of murine cell DNA polymerase mediates polyomavirus DNA replication in vitro.
1994,
Pubmed
Zahradka,
A shuttle mechanism for DNA-protein interactions. The regulation of poly(ADP-ribose) polymerase.
1982,
Pubmed
Zdzienicka,
A Chinese hamster ovary cell mutant (EM-C11) with sensitivity to simple alkylating agents and a very high level of sister chromatid exchanges.
1992,
Pubmed
de Murcia,
Poly(ADP-ribose) polymerase: a molecular nick-sensor.
1994,
Pubmed
