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Rouxs Arch Dev Biol
1992 Dec 01;2021:4-9. doi: 10.1007/BF00364591.
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Expression and distribution of SPARC in early Xenopus laevis embryos.
Ringuette M
,
Drysdale T
,
Liu F
.
???displayArticle.abstract??? SPARC (Secreted Protein, Acidic, Rich in Cysteine) is a highly conserved, calcium-binding, extracellular matrix protein. To investigate its role in early embryogenesis, we examined its tissue distribution in early Xenopus embryos. SPARC mRNA transcripts were detectable by Northern blot analysis beginning at the early neurula stage. SPARC transcripts then rapidly accumulated, reaching their highest embryonic level at the tailbud stage. The levels of SPARC mRNA were similar in dorsalized (LiCl-treated) and ventralized (UV-treated) embryos, indicating embryonic expression of SPARC mRNA was not obviously altered by changes in pattern formation. Immunofluorescence staining showed SPARC was an abundant protein in tissues of mesodermal and ectodermal origin in tailbud embryos, with the exception of the notochord. Of particular interest was the distribution of SPARC within the intersomitic clefts during somitogenesis. The widespread distribution of SPARC in early embryogenesis suggests it may play a role in the formation of a variety of tissues.
Cleveland,
Number and evolutionary conservation of alpha- and beta-tubulin and cytoplasmic beta- and gamma-actin genes using specific cloned cDNA probes.
1980, Pubmed
Cleveland,
Number and evolutionary conservation of alpha- and beta-tubulin and cytoplasmic beta- and gamma-actin genes using specific cloned cDNA probes.
1980,
Pubmed
Clezardin,
Complex formation of human thrombospondin with osteonectin.
1988,
Pubmed
Damjanovski,
Molecular analysis of Xenopus laevis SPARC (Secreted Protein, Acidic, Rich in Cysteine). A highly conserved acidic calcium-binding extracellular-matrix protein.
1992,
Pubmed
,
Xenbase
Domenicucci,
Characterization of porcine osteonectin extracted from foetal calvariae.
1988,
Pubmed
Drysdale,
Development of the Xenopus laevis hatching gland and its relationship to surface ectoderm patterning.
1991,
Pubmed
,
Xenbase
Dziadek,
Purification and tissue distribution of a small protein (BM-40) extracted from a basement membrane tumor.
1986,
Pubmed
Ekblom,
Cell-matrix interactions and cell adhesion during development.
1986,
Pubmed
Erickson,
Tenascin: an extracellular matrix protein prominent in specialized embryonic tissues and tumors.
1989,
Pubmed
Holland,
In vivo expression of mRNA for the Ca++-binding protein SPARC (osteonectin) revealed by in situ hybridization.
1987,
Pubmed
Jundt,
Immunocytochemical identification of osteogenic bone tumors by osteonectin antibodies.
1989,
Pubmed
Kelm,
The collagen binding specificity of bone and platelet osteonectin is related to differences in glycosylation.
1991,
Pubmed
Kelm,
Human platelet osteonectin: release, surface expression, and partial characterization.
1990,
Pubmed
Krotoski,
Distribution of integrins and their ligands in the trunk of Xenopus laevis during neural crest cell migration.
1990,
Pubmed
,
Xenbase
McNeish,
Phenotypic characterization of the transgenic mouse insertional mutation, legless.
1990,
Pubmed
Mohun,
Cell type-specific activation of actin genes in the early amphibian embryo.
,
Pubmed
,
Xenbase
Otsuka,
Biosynthesis of osteonectin by fetal porcine calvarial cells in vitro.
1984,
Pubmed
Otte,
Characterization of a maternal type VI collagen in Xenopus embryos suggests a role for collagen in gastrulation.
1990,
Pubmed
,
Xenbase
Price,
Characterization of a gamma-carboxyglutamic acid-containing protein from bone.
1976,
Pubmed
Purcell,
Developmental anomalies of Xenopus embryos following microinjection of SPARC antibodies.
1993,
Pubmed
,
Xenbase
Ringuette,
Expression of SPARC/osteonectin in tissues of bony and cartilaginous vertebrates.
1991,
Pubmed
,
Xenbase
Romberg,
Inhibition of hydroxyapatite crystal growth by bone-specific and other calcium-binding proteins.
1986,
Pubmed
STEEDMAN,
Polyester wax; a new ribboning embedding medium for histology.
1957,
Pubmed
Sage,
Extracellular proteins that modulate cell-matrix interactions. SPARC, tenascin, and thrombospondin.
1991,
Pubmed
Sage,
Distribution of the calcium-binding protein SPARC in tissues of embryonic and adult mice.
1989,
Pubmed
Sage,
SPARC, a secreted protein associated with cellular proliferation, inhibits cell spreading in vitro and exhibits Ca+2-dependent binding to the extracellular matrix.
1989,
Pubmed
Termine,
Osteonectin, a bone-specific protein linking mineral to collagen.
1981,
Pubmed
Towbin,
Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
1979,
Pubmed
