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Mech Dev January 1, 2017; 147 28-36.

The RNF146 E3 ubiquitin ligase is required for the control of Wnt signaling and body pattern formation in Xenopus.

Zhu X , Xing R , Tan R , Dai R , Tao Q .

The RING finger protein Rnf146 encodes an E3 ubiquitin ligase capable of targeting poly-ADP-ribosylated substrates for proteasomal degradation. Rnf146 has been identified as a critical regulator of Axin1 and thus of Wnt/β-catenin signaling. However its physiological significance in vertebrate embryonic development remains to be demonstrated. In this study, we take advantages of early Xenopus embryos to demonstrate that Rnf146 is essential for embryonic pattern formation. Depletion of zygotic Rnf146 using a translation blocking morpholino oligo (MO) results in anteriorized development and increased expression the anterior marker gene Otx2, consistent the notion that Rnf146 is a positive regulator of Wnt/β-catenin signaling through negatively regulating Axin1 expression. This notion is further supported by examination of the role of maternal Rnf146 in the context of Spemann organizer formation and dorsal axis development. Depletion of maternal Rnf146 using an antisense oligodeoxynucleic acid (ODN) leads to ventralized development and diminished expression of organizer genes. Together, we have provided evidence for the first time that Rnf146 is a critical regulator of embryonic pattern formation in vertebrates.

PubMed ID: 28807725
Article link: Mech Dev

Species referenced: Xenopus laevis
Genes referenced: axin1 bag3 ctnnb1 egr2 gsk3b hoxb9 myc nodal3.1 odc1 otx2 rnf146 sia1 tnks
Morpholinos: rnf146 MO2

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