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Development
2017 Apr 15;1448:1412-1424. doi: 10.1242/dev.143578.
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FGF and canonical Wnt signaling cooperate to induce paraxial mesoderm from tailbud neuromesodermal progenitors through regulation of a two-step epithelial to mesenchymal transition.
Goto H
,
Kimmey SC
,
Row RH
,
Matus DQ
,
Martin BL
.
???displayArticle.abstract??? Mesoderm induction begins during gastrulation. Recent evidence from several vertebrate species indicates that mesoderm induction continues after gastrulation in neuromesodermal progenitors (NMPs) within the posteriormost embryonic structure, the tailbud. It is unclear to what extent the molecular mechanisms of mesoderm induction are conserved between gastrula and post-gastrula stages of development. Fibroblast growth factor (FGF) signaling is required for mesoderm induction during gastrulation through positive transcriptional regulation of the T-box transcription factor brachyury We find in zebrafish that FGF is continuously required for paraxial mesoderm (PM) induction in post-gastrula NMPs. FGF signaling represses the NMP markers brachyury (ntla) and sox2 through regulation of tbx16 and msgn1, thereby committing cells to a PM fate. FGF-mediated PM induction in NMPs functions in tight coordination with canonical Wnt signaling during the epithelial to mesenchymal transition (EMT) from NMP to mesodermal progenitor. Wnt signaling initiates EMT, whereas FGF signaling terminates this event. Our results indicate that germ layer induction in the zebrafish tailbud is not a simple continuation of gastrulation events.
Acloque,
Epithelial-mesenchymal transitions: the importance of changing cell state in development and disease.
2009, Pubmed
Acloque,
Epithelial-mesenchymal transitions: the importance of changing cell state in development and disease.
2009,
Pubmed
Akai,
FGF-dependent Notch signaling maintains the spinal cord stem zone.
2005,
Pubmed
Amaya,
Expression of a dominant negative mutant of the FGF receptor disrupts mesoderm formation in Xenopus embryos.
1991,
Pubmed
,
Xenbase
Amaya,
FGF signalling in the early specification of mesoderm in Xenopus.
1993,
Pubmed
,
Xenbase
Beck,
Development of the vertebrate tailbud.
2015,
Pubmed
,
Xenbase
Bertrand,
Amphioxus FGF signaling predicts the acquisition of vertebrate morphological traits.
2011,
Pubmed
Bouldin,
Wnt signaling and tbx16 form a bistable switch to commit bipotential progenitors to mesoderm.
2015,
Pubmed
Cerdà,
Zebrafish vimentin: molecular characterization, assembly properties and developmental expression.
1998,
Pubmed
Ciruna,
FGF signaling regulates mesoderm cell fate specification and morphogenetic movement at the primitive streak.
2001,
Pubmed
Darras,
The BMP signaling pathway is required together with the FGF pathway for notochord induction in the ascidian embryo.
2001,
Pubmed
Delaune,
Neural induction in Xenopus requires early FGF signalling in addition to BMP inhibition.
2005,
Pubmed
,
Xenbase
Deng,
Murine FGFR-1 is required for early postimplantation growth and axial organization.
1994,
Pubmed
,
Xenbase
Dorey,
FGF signalling: diverse roles during early vertebrate embryogenesis.
2010,
Pubmed
,
Xenbase
Dubrulle,
FGF signaling controls somite boundary position and regulates segmentation clock control of spatiotemporal Hox gene activation.
2001,
Pubmed
Edgar,
MUSCLE: multiple sequence alignment with high accuracy and high throughput.
2004,
Pubmed
Fior,
The differentiation and movement of presomitic mesoderm progenitor cells are controlled by Mesogenin 1.
2012,
Pubmed
Fletcher,
The role of FGF signaling in the establishment and maintenance of mesodermal gene expression in Xenopus.
2008,
Pubmed
,
Xenbase
Futterman,
Evidence for partial epithelial-to-mesenchymal transition (pEMT) and recruitment of motile blastoderm edge cells during avian epiboly.
2011,
Pubmed
,
Xenbase
Garriock,
Lineage tracing of neuromesodermal progenitors reveals novel Wnt-dependent roles in trunk progenitor cell maintenance and differentiation.
2015,
Pubmed
Gouti,
In vitro generation of neuromesodermal progenitors reveals distinct roles for wnt signalling in the specification of spinal cord and paraxial mesoderm identity.
2014,
Pubmed
Grande,
Snail1-induced partial epithelial-to-mesenchymal transition drives renal fibrosis in mice and can be targeted to reverse established disease.
2015,
Pubmed
Green,
FGF signaling induces mesoderm in the hemichordate Saccoglossus kowalevskii.
2013,
Pubmed
Griffin,
Molecular identification of spadetail: regulation of zebrafish trunk and tail mesoderm formation by T-box genes.
1998,
Pubmed
,
Xenbase
Griffin,
Analysis of FGF function in normal and no tail zebrafish embryos reveals separate mechanisms for formation of the trunk and the tail.
1995,
Pubmed
Henrique,
Neuromesodermal progenitors and the making of the spinal cord.
2015,
Pubmed
Herrmann,
Identification of a nonapeptide motif in the vimentin head domain involved in intermediate filament assembly.
1992,
Pubmed
,
Xenbase
Ho,
Cell-autonomous action of zebrafish spt-1 mutation in specific mesodermal precursors.
,
Pubmed
,
Xenbase
Hubaud,
Signalling dynamics in vertebrate segmentation.
2014,
Pubmed
Imai,
Early embryonic expression of FGF4/6/9 gene and its role in the induction of mesenchyme and notochord in Ciona savignyi embryos.
2002,
Pubmed
Isaacs,
eFGF regulates Xbra expression during Xenopus gastrulation.
1994,
Pubmed
,
Xenbase
Jolly,
Implications of the Hybrid Epithelial/Mesenchymal Phenotype in Metastasis.
2015,
Pubmed
Jurberg,
Compartment-dependent activities of Wnt3a/β-catenin signaling during vertebrate axial extension.
2014,
Pubmed
Kiecker,
Molecular specification of germ layers in vertebrate embryos.
2016,
Pubmed
Kim,
Role of the FGF and MEK signaling pathway in the ascidian embryo.
2001,
Pubmed
Kimelman,
Mesoderm induction: from caps to chips.
2006,
Pubmed
,
Xenbase
Kimelman,
Anterior-posterior patterning in early development: three strategies.
2012,
Pubmed
Kimelman,
The presence of fibroblast growth factor in the frog egg: its role as a natural mesoderm inducer.
1988,
Pubmed
,
Xenbase
Kimelman,
Tales of Tails (and Trunks): Forming the Posterior Body in Vertebrate Embryos.
2016,
Pubmed
Kimelman,
Synergistic induction of mesoderm by FGF and TGF-beta and the identification of an mRNA coding for FGF in the early Xenopus embryo.
1987,
Pubmed
,
Xenbase
Kondoh,
Axial stem cells deriving both posterior neural and mesodermal tissues during gastrulation.
2012,
Pubmed
Latinkić,
The Xenopus Brachyury promoter is activated by FGF and low concentrations of activin and suppressed by high concentrations of activin and by paired-type homeodomain proteins.
1997,
Pubmed
,
Xenbase
Lawton,
Regulated tissue fluidity steers zebrafish body elongation.
2013,
Pubmed
Lee,
Fgf signaling instructs position-dependent growth rate during zebrafish fin regeneration.
2005,
Pubmed
Lowery,
Intermediate Filaments Play a Pivotal Role in Regulating Cell Architecture and Function.
2015,
Pubmed
Lu,
MicroRNA-based regulation of epithelial-hybrid-mesenchymal fate determination.
2013,
Pubmed
Mandal,
Modeling continuum of epithelial mesenchymal transition plasticity.
2016,
Pubmed
Manning,
Tbx16 and Msgn1 are required to establish directional cell migration of zebrafish mesodermal progenitors.
2015,
Pubmed
Marques,
Reiterative roles for FGF signaling in the establishment of size and proportion of the zebrafish heart.
2008,
Pubmed
Martin,
Factors that coordinate mesoderm specification from neuromesodermal progenitors with segmentation during vertebrate axial extension.
2016,
Pubmed
Martin,
Wnt signaling and the evolution of embryonic posterior development.
2009,
Pubmed
Martin,
Regulation of canonical Wnt signaling by Brachyury is essential for posterior mesoderm formation.
2008,
Pubmed
Martin,
Brachyury establishes the embryonic mesodermal progenitor niche.
2010,
Pubmed
Martin,
Canonical Wnt signaling dynamically controls multiple stem cell fate decisions during vertebrate body formation.
2012,
Pubmed
,
Xenbase
Mathis,
FGF receptor signalling is required to maintain neural progenitors during Hensen's node progression.
2001,
Pubmed
McCoon,
SpFGFR, a new member of the fibroblast growth factor receptor family, is developmentally regulated during early sea urchin development.
1996,
Pubmed
McCoon,
Sea urchin FGFR muscle-specific expression: posttranscriptional regulation in embryos and adults.
1998,
Pubmed
Mitrani,
Fibroblast growth factor during mesoderm induction in the early chick embryo.
1990,
Pubmed
,
Xenbase
Miya,
An Ets transcription factor, HrEts, is target of FGF signaling and involved in induction of notochord, mesenchyme, and brain in ascidian embryos.
2003,
Pubmed
Nieto,
EMT: 2016.
2016,
Pubmed
Olivera-Martinez,
Loss of FGF-dependent mesoderm identity and rise of endogenous retinoid signalling determine cessation of body axis elongation.
2012,
Pubmed
Ramkumar,
Crumbs2 promotes cell ingression during the epithelial-to-mesenchymal transition at gastrulation.
2016,
Pubmed
Revenu,
EMT 2.0: shaping epithelia through collective migration.
2009,
Pubmed
Ronquist,
MrBayes 3: Bayesian phylogenetic inference under mixed models.
2003,
Pubmed
Row,
The zebrafish tailbud contains two independent populations of midline progenitor cells that maintain long-term germ layer plasticity and differentiate in response to local signaling cues.
2016,
Pubmed
Row,
Completion of the epithelial to mesenchymal transition in zebrafish mesoderm requires Spadetail.
2011,
Pubmed
Röttinger,
FGF signals guide migration of mesenchymal cells, control skeletal morphogenesis [corrected] and regulate gastrulation during sea urchin development.
2008,
Pubmed
Schulte-Merker,
Mesoderm formation in response to Brachyury requires FGF signalling.
1995,
Pubmed
,
Xenbase
Slack,
Mesoderm induction in early Xenopus embryos by heparin-binding growth factors.
,
Pubmed
,
Xenbase
Steventon,
Species-specific contribution of volumetric growth and tissue convergence to posterior body elongation in vertebrates.
2016,
Pubmed
Stulberg,
Crosstalk between Fgf and Wnt signaling in the zebrafish tailbud.
2012,
Pubmed
Tian,
Coupled reversible and irreversible bistable switches underlying TGFβ-induced epithelial to mesenchymal transition.
2013,
Pubmed
Tsakiridis,
Distinct Wnt-driven primitive streak-like populations reflect in vivo lineage precursors.
2014,
Pubmed
Veldman,
Transdifferentiation of fast skeletal muscle into functional endothelium in vivo by transcription factor Etv2.
2013,
Pubmed
Wymeersch,
Position-dependent plasticity of distinct progenitor types in the primitive streak.
2016,
Pubmed
Yabe,
Mesogenin causes embryonic mesoderm progenitors to differentiate during development of zebrafish tail somites.
2012,
Pubmed
Yamaguchi,
fgfr-1 is required for embryonic growth and mesodermal patterning during mouse gastrulation.
1994,
Pubmed
Yasuo,
FGF8/17/18 functions together with FGF9/16/20 during formation of the notochord in Ciona embryos.
2007,
Pubmed
Ye,
Epithelial-Mesenchymal Plasticity: A Central Regulator of Cancer Progression.
2015,
Pubmed
