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FASEB J
2018 Jan 01;321:431-439. doi: 10.1096/fj.201700424R.
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EVI and MDS/EVI are required for adult intestinal stem cell formation during postembryonic vertebrate development.
Okada M
,
Shi YB
.
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The gene ectopic viral integration site 1 (EVI) and its variant myelodysplastic syndrome 1 (MDS)/EVI encode zinc-finger proteins that have been recognized as important oncogenes in various types of cancer. In contrast to the established role of EVI and MDS/EVI in cancer development, their potential function during vertebrate postembryonic development, especially in organ-specific adult stem cells, is unclear. Amphibian metamorphosis is strikingly similar to postembryonic development around birth in mammals, with both processes taking place when plasma thyroid hormone (T3) levels are high. Using the T3-dependent metamorphosis in Xenopus tropicalis as a model, we show here that high levels of EVI and MDS/EVI are expressed in the intestine at the climax of metamorphosis and are induced by T3. By using the transcription activator-like effector nuclease gene editing technology, we have knocked out both EVI and MDS/EVI and have shown that EVI and MDS/EVI are not essential for embryogenesis and premetamorphosis in X. tropicalis On the other hand, knocking out EVI and MDS/EVI causes severe retardation in the growth and development of the tadpoles during metamorphosis and leads to tadpole lethality at the climax of metamorphosis. Furthermore, the homozygous-knockout animals have reduced adult intestinal epithelial stem cell proliferation at the end of metamorphosis (for the few that survive through metamorphosis) or during T3-induced metamorphosis. These findings reveal a novel role of EVI and/or MDS/EVI in regulating the formation and/or proliferation of adult intestinal adult stem cells during postembryonic development in vertebrates.-Okada, M., Shi, Y.-B. EVI and MDS/EVI are required for adult intestinal stem cell formation during postembryonic vertebrate development.
Bard-Chapeau,
Ecotopic viral integration site 1 (EVI1) regulates multiple cellular processes important for cancer and is a synergistic partner for FOS protein in invasive tumors.
2012, Pubmed
Bard-Chapeau,
Ecotopic viral integration site 1 (EVI1) regulates multiple cellular processes important for cancer and is a synergistic partner for FOS protein in invasive tumors.
2012,
Pubmed
Gao,
The Evi1, microRNA-143, K-Ras axis in colon cancer.
2011,
Pubmed
Goyama,
Evi-1 is a critical regulator for hematopoietic stem cells and transformed leukemic cells.
2008,
Pubmed
Hasebe,
Thyroid hormone-induced cell-cell interactions are required for the development of adult intestinal stem cells.
2013,
Pubmed
,
Xenbase
Ishizuya-Oka,
Evolutionary insights into postembryonic development of adult intestinal stem cells.
2011,
Pubmed
Ishizuya-Oka,
Regulation of adult intestinal epithelial stem cell development by thyroid hormone during Xenopus laevis metamorphosis.
2007,
Pubmed
,
Xenbase
Kashiwagi,
Xenopus tropicalis: an ideal experimental animal in amphibia.
2010,
Pubmed
,
Xenbase
Kataoka,
Evi1 is essential for hematopoietic stem cell self-renewal, and its expression marks hematopoietic cells with long-term multilineage repopulating activity.
2011,
Pubmed
Kataoka,
Ecotropic viral integration site 1, stem cell self-renewal and leukemogenesis.
2012,
Pubmed
Korinek,
Constitutive transcriptional activation by a beta-catenin-Tcf complex in APC-/- colon carcinoma.
1997,
Pubmed
Kumano,
The role of Runx1/AML1 and Evi-1 in the regulation of hematopoietic stem cells.
2010,
Pubmed
Lei,
Generation of gene disruptions by transcription activator-like effector nucleases (TALENs) in Xenopus tropicalis embryos.
2013,
Pubmed
,
Xenbase
Luu,
Differential regulation of two histidine ammonia-lyase genes during Xenopus development implicates distinct functions during thyroid hormone-induced formation of adult stem cells.
2013,
Pubmed
,
Xenbase
MACDONALD,
CELL PROLIFERATION AND MIGRATION IN THE STOMACH, DUODENUM, AND RECTUM OF MAN: RADIOAUTOGRAPHIC STUDIES.
1964,
Pubmed
Miller,
Tissue-specific upregulation of MDS/EVI gene transcripts in the intestine by thyroid hormone during Xenopus metamorphosis.
2013,
Pubmed
,
Xenbase
Morishita,
Activation of EVI1 gene expression in human acute myelogenous leukemias by translocations spanning 300-400 kilobases on chromosome band 3q26.
1992,
Pubmed
Nakajima,
Targeted gene disruption in the Xenopus tropicalis genome using designed TALE nucleases.
2013,
Pubmed
,
Xenbase
Nanjundan,
Amplification of MDS1/EVI1 and EVI1, located in the 3q26.2 amplicon, is associated with favorable patient prognosis in ovarian cancer.
2007,
Pubmed
Okada,
Expression of the amelogenin gene in the skin of Xenopus tropicalis.
2013,
Pubmed
,
Xenbase
Okada,
Molecular and cytological analyses reveal distinct transformations of intestinal epithelial cells during Xenopus metamorphosis.
2015,
Pubmed
,
Xenbase
Okada,
Direct Activation of Amidohydrolase Domain-Containing 1 Gene by Thyroid Hormone Implicates a Role in the Formation of Adult Intestinal Stem Cells During Xenopus Metamorphosis.
2015,
Pubmed
,
Xenbase
Patel,
Control of EVI-1 oncogene expression in metastatic breast cancer cells through microRNA miR-22.
2011,
Pubmed
Shi,
The development of the adult intestinal stem cells: Insights from studies on thyroid hormone-dependent amphibian metamorphosis.
2011,
Pubmed
,
Xenbase
Shi,
Biphasic intestinal development in amphibians: embryogenesis and remodeling during metamorphosis.
1996,
Pubmed
,
Xenbase
Sterling,
Cytological and morphological analyses reveal distinct features of intestinal development during Xenopus tropicalis metamorphosis.
2012,
Pubmed
,
Xenbase
Sun,
Thyroid hormone regulation of adult intestinal stem cell development: mechanisms and evolutionary conservations.
2012,
Pubmed
,
Xenbase
Sun,
Expression profiling of intestinal tissues implicates tissue-specific genes and pathways essential for thyroid hormone-induced adult stem cell development.
2013,
Pubmed
,
Xenbase
Sun,
Activation of Sox3 gene by thyroid hormone in the developing adult intestinal stem cell during Xenopus metamorphosis.
2014,
Pubmed
,
Xenbase
Tata,
Gene expression during metamorphosis: an ideal model for post-embryonic development.
1993,
Pubmed
Van Campenhout,
Evi1 is specifically expressed in the distal tubule and duct of the Xenopus pronephros and plays a role in its formation.
2006,
Pubmed
,
Xenbase
Wen,
Unliganded thyroid hormone receptor α controls developmental timing in Xenopus tropicalis.
2015,
Pubmed
,
Xenbase
Wen,
Histone methyltransferase Dot1L plays a role in postembryonic development in Xenopus tropicalis.
2015,
Pubmed
,
Xenbase
Wieser,
The oncogene and developmental regulator EVI1: expression, biochemical properties, and biological functions.
2007,
Pubmed
Zhang,
PR-domain-containing Mds1-Evi1 is critical for long-term hematopoietic stem cell function.
2011,
Pubmed
van Es,
Notch/gamma-secretase inhibition turns proliferative cells in intestinal crypts and adenomas into goblet cells.
2005,
Pubmed
van der Flier,
Stem cells, self-renewal, and differentiation in the intestinal epithelium.
2009,
Pubmed
