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Kir1.1 (ROMK) and Kv7.1 (KCNQ1/KvLQT1) are essential for normal gastric acid secretion: importance of functional Kir1.1.
Vucic E
,
Alfadda T
,
MacGregor GG
,
Dong K
,
Wang T
,
Geibel JP
.
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Potassium channels comprise the apical leak pathway supplying extracellular K(+) for exchange with protons by the gastric H(+), K(+)-ATPase and provide potential therapeutic targets for inhibiting gastric acid secretion. The Kir1.1 (ROMK) potassium channel mediates the high capacity K(+) recycling necessary for NaCl reabsorption in the thick ascending limb of the kidney, and this channel exhibits functional and regulatory characteristic well suited for K(+) recycling by gastric parietal cells. We report here that Kir1.1 channels are required for gastric acid secretion and that this channel participates with Kv7.1 (KCNQ1/KvLQT1) in the potassium recycling process. We show that Kir1.1 colocalizes with the β-subunit of H(+), K(+)-ATPase in gastric parietal cells of Kir1.1 wild-type mice. In Kir1.1-deficient mice, gastric mucosal morphology, as well as parietal cell number, proliferation index, and ultrastructure were normal but secretagogue-stimulated gastric acid secretion in whole stomach and perfused gastric glands was absent. Luminal application of potassium-restored acid secretion in perfused gastric glands from Kir1.1-deficient as well as barium-blocked wild-type mice. In wild-type mice, both luminal Tertiapin-Q, an inhibitor of Kir1.1, as well as XE991, an inhibitor of Kv7.1, reduced proton secretion. We propose that Kir1.1 and Kv7.1 channels collaborate in potassium and current recycling across the apical pole of parietal cells.
Asano,
Molecular and cellular regulation of the gastric proton pump.
2004, Pubmed
Asano,
Molecular and cellular regulation of the gastric proton pump.
2004,
Pubmed
Boim,
ROMK inwardly rectifying ATP-sensitive K+ channel. II. Cloning and distribution of alternative forms.
1995,
Pubmed
,
Xenbase
Breton,
Depletion of intercalated cells from collecting ducts of carbonic anhydrase II-deficient (CAR2 null) mice.
1995,
Pubmed
Buharalioğlu,
The reactivity of serotonin, acetylcholine and kcl-induced contractions to relaxant agents in the rat gastric fundus.
2002,
Pubmed
Cuppoletti,
Regulation of gastric acid secretion via modulation of a chloride conductance.
1984,
Pubmed
Dedek,
Colocalization of KCNQ1/KCNE channel subunits in the mouse gastrointestinal tract.
2001,
Pubmed
Elso,
Heightened susceptibility to chronic gastritis, hyperplasia and metaplasia in Kcnq1 mutant mice.
2004,
Pubmed
Forte,
K+ channels in the secretory membrane of the parietal cell. focus on "Gastric parietal cell secretory membrane contains PKA- and acid-activated Kir2.1 K+ channels".
2004,
Pubmed
Forte,
Membrane and protein recycling associated with gastric HCl secretion.
1990,
Pubmed
Fujita,
Expression of Ca(2+)-activated K(+) channels, SK3, in the interstitial cells of Cajal in the gastrointestinal tract.
2001,
Pubmed
Fujita,
Specific localization of an inwardly rectifying K(+) channel, Kir4.1, at the apical membrane of rat gastric parietal cells; its possible involvement in K(+) recycling for the H(+)-K(+)-pump.
2002,
Pubmed
Geibel,
The stomach divalent ion-sensing receptor scar is a modulator of gastric acid secretion.
2001,
Pubmed
Giebisch,
New aspects of renal potassium transport.
2003,
Pubmed
Goswami,
Anti-ulcer activity of cromakalim (BRL 34915), a potassium-channel opener, against experimentally induced gastric and duodenal ulcers in rats and guinea-pigs.
1997,
Pubmed
Grahammer,
The cardiac K+ channel KCNQ1 is essential for gastric acid secretion.
2001,
Pubmed
Hebert,
Bartter syndrome.
2003,
Pubmed
Hebert,
Molecular diversity and regulation of renal potassium channels.
2005,
Pubmed
Hebert,
Structure and functional properties of an inwardly rectifying ATP-regulated K+ channel from rat kidney.
1994,
Pubmed
Hebert,
Structure and function of the low conductance KATP channel, ROMK.
1997,
Pubmed
Heitzmann,
Heteromeric KCNE2/KCNQ1 potassium channels in the luminal membrane of gastric parietal cells.
2004,
Pubmed
Ho,
Cloning and expression of an inwardly rectifying ATP-regulated potassium channel.
1993,
Pubmed
,
Xenbase
Iwata,
Functional evidence linking potassium channels and afferent nerve-mediated mucosal protection in rat stomach.
1997,
Pubmed
Jain,
Gene expression profiling of gastrin target genes in parietal cells.
2006,
Pubmed
Jin,
Mechanisms of inward-rectifier K+ channel inhibition by tertiapin-Q.
1999,
Pubmed
,
Xenbase
Jin,
A novel high-affinity inhibitor for inward-rectifier K+ channels.
1998,
Pubmed
,
Xenbase
Kanjhan,
Tertiapin-Q blocks recombinant and native large conductance K+ channels in a use-dependent manner.
2005,
Pubmed
,
Xenbase
Kirchhoff,
Demonstration of a functional apical sodium hydrogen exchanger in isolated rat gastric glands.
2003,
Pubmed
Lambrecht,
Identification of the K efflux channel coupled to the gastric H-K-ATPase during acid secretion.
2005,
Pubmed
Lee,
Targeted disruption of the Kvlqt1 gene causes deafness and gastric hyperplasia in mice.
2000,
Pubmed
Lorenz,
Impaired renal NaCl absorption in mice lacking the ROMK potassium channel, a model for type II Bartter's syndrome.
2002,
Pubmed
Lu,
Absence of small conductance K+ channel (SK) activity in apical membranes of thick ascending limb and cortical collecting duct in ROMK (Bartter's) knockout mice.
2002,
Pubmed
Malinowska,
Gastric parietal cell secretory membrane contains PKA- and acid-activated Kir2.1 K+ channels.
2004,
Pubmed
,
Xenbase
Miller,
The unique ultrastructure of secretory membranes in gastric parietal cells depends upon the presence of H+, K+ -ATPase.
2002,
Pubmed
Park,
Molecular cloning of a pore-forming subunit (Kir6.2 gene) of the ATP-sensitive potassium channel in the bullfrog, Rana catesbeiana Shaw.
2002,
Pubmed
Peskar,
Role of ATP-sensitive potassium channels in prostaglandin-mediated gastroprotection in the rat.
2002,
Pubmed
Prinz,
Acid secretion and the H,K ATPase of stomach.
1992,
Pubmed
Roepke,
The KCNE2 potassium channel ancillary subunit is essential for gastric acid secretion.
2006,
Pubmed
Sachs,
Gastric acid secretion: activation and inhibition.
1994,
Pubmed
Sachs,
Physiology of the parietal cell and therapeutic implications.
2003,
Pubmed
Spicer,
Stomachs of mice lacking the gastric H,K-ATPase alpha -subunit have achlorhydria, abnormal parietal cells, and ciliated metaplasia.
2000,
Pubmed
Vallon,
KCNQ1-dependent transport in renal and gastrointestinal epithelia.
2005,
Pubmed
Waisbren,
Unusual permeability properties of gastric gland cells.
1994,
Pubmed
Waisbren,
Luminal perfusion of isolated gastric glands.
1994,
Pubmed
Waldegger,
Heartburn: cardiac potassium channels involved in parietal cell acid secretion.
2003,
Pubmed
Wang,
Molecular basis for differential sensitivity of KCNQ and I(Ks) channels to the cognitive enhancer XE991.
2000,
Pubmed
,
Xenbase
Wang,
Phospholipase A2 is involved in mediating the effect of extracellular Ca2+ on apical K+ channels in rat TAL.
1997,
Pubmed
Xu,
Localization of the ROMK protein on apical membranes of rat kidney nephron segments.
1997,
Pubmed
Zaczek,
Two new potent neurotransmitter release enhancers, 10,10-bis(4-pyridinylmethyl)-9(10H)-anthracenone and 10,10-bis(2-fluoro-4-pyridinylmethyl)-9(10H)-anthracenone: comparison to linopirdine.
1998,
Pubmed
Zhang,
Opposing roles of K(+) and Cl(-) channels in maintenance of opossum lower esophageal sphincter tone.
2000,
Pubmed
