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Data Brief
2017 Nov 04;15:970-974. doi: 10.1016/j.dib.2017.10.056.
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Trimethylation of Histone 3 lysine 27 (H3K27me3) ChIP-PCR and transcriptional expression data of Ef1-alpha, cyp26A, HoxC10, HoxD10 and HoxD11 in the Xenopus XTC cell line.
Vieira W
,
Sahin H
,
Wells K
,
McCusker C
.
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Trimethylation of Histone 3 lysine 27 (H3K27me3) is a chromatin modification that is associated with transcriptional repression (Cao et al., 2002; Sarma et al., 2008; Pengelly et al., 2013) [1], [2], [3]. In this article we performed anti-H3K27me3 Chromosomal Immunoprecipitation (ChIP-PCR), to detect the abundance of H3K27me3 marks on Ef1-alpha, cyp26A, HoxC10, HoxD10 and HoxD11 in the Xenopus XTC cell line. We also performed RT-PCR for these genes to determine whether their expression is detectable in the XTC cell culture. The data we present here are the fold enrichment of Ef1-alpha, cyp26A, HoxC10, HoxD10 and HoxD11 on anti-H3K27me3 ChIP compared to no antibody controls. We also present RT-PCR data on the above listed genes.
Fig. 1. Anti-H3K27me3 ChIP-PCR data for Ef1-alpha, Cyp26A, HoxC10, HoxD10, and HoxD11: (Top panel) Fold enrichment of the abundance of regions of Ef1-alpha, cyp26A, HoxC10, HoxD10 and HoxD11 amplified by different PCR primer sets (A, B, D, or D) for each gene. Fold enrichment was quantified as a ratio of the abundance of each amplimer in the PCR reactions from the ChIP with or without the anti-H3K27me3 antibody. (Lower panel) Schematic representing the location of each primer set that was used for each gene relative to the transcription start site (TSS).
Fig. 2. RT-PCR data for Ef1-alpha, cyp26A, HoxC10, HoxD10 and HoxD11 in XTC cells: A semi-quantitative method of analysis was used to determine the relative transcriptional expression of specific genes in the XTC cell line. Regions of the Ef1-alpha, cyp26A, HoxC10, HoxD10 and HoxD11 transcripts were amplified by different PCR primer sets from cDNA libraries generated from unmanipulated XTC cells.
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