Click here to close Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari.
XB-ART-54344
Methods Mol Biol 2018 Jan 01;1713:45-55. doi: 10.1007/978-1-4939-7507-5_4.
Show Gene links Show Anatomy links

GLUT Characterization Using Frog Xenopus laevis Oocytes.

Long W , O'Neill D , Cheeseman CI .


???displayArticle.abstract???
Xenopus laevis oocytes are a useful heterologous expression system for expressing glucose transporters (GLUTs) and examining their functions. In this chapter, we provide a detailed protocol on oocyte extraction and preparation for GLUT9 protein expression. Furthermore, we describe the determination of GLUT9 overexpression level by biotinylation and Western blotting analysis. Finally, we also describe how GLUT9-expressing oocytes can be used to measure urate kinetics by radioisotopes as well as two-microelectrode voltage clamping techniques.

???displayArticle.pubmedLink??? 29218516
???displayArticle.link??? Methods Mol Biol


Species referenced: Xenopus laevis
Genes referenced: slc2a9